A5083856153- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- interferon and immune responses
- Amino Acid Enzymes and Metabolism
- Aquatic Invertebrate Ecology and Behavior
- Antimicrobial Peptides and Activities
- Fish Ecology and Management Studies
- Freshwater macroinvertebrate diversity and ecology
- Bacterial biofilms and quorum sensing
- Magnesium in Health and Disease
- Advanced Neuroimaging Techniques and Applications
- Aluminum toxicity and tolerance in plants and animals
- Chemotherapy-induced cardiotoxicity and mitigation
- Clostridium difficile and Clostridium perfringens research
- Biochemical and Molecular Research
- Neuroscience and Neuropharmacology Research
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Ion channel regulation and function
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Genetic Neurodegenerative Diseases
University of St Andrews
2023-2024
University of Göttingen
2016-2022
Universidade Estadual do Centro-Oeste
2017
Abstract Many bacteria use cyclic di-AMP as a second messenger to control potassium and osmotic homeostasis. In Bacillus subtilis , several c-di-AMP binding proteins RNA molecules have been identified. Most of these targets play role in controlling uptake export. addition, binds two conserved target unknown function, DarA DarB, that exclusively consist the domain. Here, we investigate function c-di-AMP-binding protein DarB B. which consists cystathionine-beta synthase (CBS) domains. We an...
Osmotic stress is a significant physical challenge for free-living cells. Cells from all three domains of life maintain viability during osmotic by tightly regulating the major cellular osmolyte potassium (K
The carbon sensor SbtB perceives diurnal oscillation of c-di-AMP to control glycogen synthesis and nighttime survival.
In order to adjust changing environmental conditions, bacteria use nucleotide second messengers transduce external signals and translate them into a specific cellular response. Cyclic di-adenosine monophosphate (c-di-AMP) is the only known essential messenger. addition well-established role of this messenger in control potassium homeostasis, we observed that glutamate as toxic for c-di-AMP-free strain Gram-positive model bacterium Bacillus subtilis . work, isolated suppressor mutants allow...
In Bacillus subtilis and other Gram-positive bacteria, cyclic di-AMP is an essential second messenger that signals potassium availability by binding to a variety of proteins. some c-di-AMP also binds the pyruvate carboxylase inhibit its activity. We have discovered in B. target protein DarB, rather than itself, specifically both vivo vitro. This interaction stimulates activity enzyme, as demonstrated vitro enzyme assays metabolite determinations. Both activation require apo-DarB are...
Results of experiments in which regional neuronal activity is revealed by a 2-[3H]deoxy-D-glucose (3H-2-DG)-paraffin section-emulsion autoradiography method are described. The trigeminal pathway freely behaving mice was activated differentially selective patterns whisker removal. One hour after injection concentrated 3H-2-DG, the animals were perfused systemically with periodate/lysine/paraformaldehyde mixture (McLean, I. W., and P. K. Nakane (1974) J. Histochem. Cytochem. 22: 1077-1083),...
Abstract Prokaryotic antiviral defence systems are frequently toxic for host cells and stringent regulation is required to ensure survival fitness. These must be readily available in case of infection but tightly controlled prevent activation an unnecessary cellular response. Here we investigate how the bacterial cyclic oligonucleotide-based antiphage signalling system (CBASS) uses its intrinsic protein modification regulate nucleotide cyclase. By integrating a type II CBASS from Bacillus...
ABSTRACT Potassium and glutamate are the major cation anion, respectively, in every living cell. Due to high concentrations of both ions, cytoplasm all cells can be regarded as a potassium solution. This implies that ions need balanced. While control uptake by is well established for eukaryotic cells, much less known about mechanisms link homeostasis availability bacteria. Here, we have discovered strongly decreases minimal external concentration required highly abundant Bacillus subtilis...
The Gram-positive bacterium Bacillus subtilis uses serine not only as a building block for proteins but also an important precursor in many anabolic reactions. Moreover, lack of results the initiation biofilm formation. However, excess inhibits growth B. subtilis. To unravel underlying mechanisms, we isolated suppressor mutants that can tolerate toxic concentrations by three targeted and non-targeted genome-wide screens. All screens well genetic complementation Escherichia coli identified so...
The bacterial second messenger c-di-AMP controls essential cellular processes, including potassium and osmolyte homeostasis. This makes synthesizing enzymes components involved in signal transduction intriguing as potential targets for drug development. receptor protein DarB of Bacillus subtilis binds the Rel triggers Rel-dependent stringent response to stress conditions; however, structural basis this trigger is unclear. Here, we report crystal structures ligand-free state complexed with...
Abstract Antiviral defence systems build the prokaryotic immune system and their proper regulation is vital for survival fitness. While it important that they are readily available in case of infection, need to be tightly controlled prevent activation an unnecessary cellular response. Here we describe how bacterial cyclic oligonucleotide-based antiphage signalling (CBASS) uses its intrinsic protein modification regulate nucleotide cyclase. By integrating a Type II CBASS from Bacillus cereus...
Due to concerns about toxicity, many elderly patients with aggressive non-Hodgkin's lymphoma (NHL) are not considered candidates for standard chemotherapy cyclophosphamide, doxorubicin, vincristine and prednisone (CHOP). The cytoprotective agent amifostine has the potential reduce toxicity when added chemotherapy. purpose of current study was examine CHOP combined in NHL. A prospective phase II performed aged 60 years older. Patients stage I/II disease received 4 cycles followed by...
Abstract Aim: Here we analyzed some basic ecological attributes (richness and density) of the benthic community in a stretch rocky river under influence small dam meter height, forming reservoir run-of-river type. Methods Sediment samples were taken with Petersen collector at three different periods sites: upstream from (site 1), close to 2) downstream 3). Organisms separated large groups Chironomidae genera. Results The particle size analysis indicated that sites 1 2 are dominated by fine...
Abstract Many bacteria use cyclic di-AMP as a second messenger to control potassium and osmotic homeostasis. In Bacillus subtilis , several c-di-AMP binding proteins RNA molecules have been identified. Most of these targets play role in controlling uptake export. addition, binds two conserved target unknown function, DarA DarB, that exclusively consist the domain. likely transduce their signal by regulatory interactions with other proteins. Here, we investigated function c-di-AMP-binding...
The moderately thermophilic bacterium Clostridium tepidiprofundi is Gram-positive and belongs to clostridial cluster I. It was isolated from a hydrothermal vent chimney. Substrates utilized by C. include casein, peptone, tryptone, yeast extract, beef starch, maltose, glucose. genome consists of one replicon (3.06 Mb).
Summary The Gram-positive bacterium Bacillus subtilis uses serine not only as building block for proteins but also an important precursor in many anabolic reactions. Moreover, a lack of results the initiation biofilm formation. However, excess inhibits growth B. . To unravel underlying mechanisms, we isolated suppressor mutants that can tolerate toxic concentrations by three targeted and non-targeted genome-wide screens. All screens well genetic complementation Escherichia coli identified so...
ABSTRACT In order to adjust changing environmental conditions, bacteria use nucleotide second messengers transduce external signals and translate them into a specific cellular response. Cyclic di-adenosine monophosphate (c-di-AMP) is the only known essential messenger. addition well-established role of this messenger in control potassium homeostasis, we observed that glutamate as toxic for c-di-AMP-free strain Gram-positive model bacterium Bacillus subtilis . work, isolated suppressor...