A5103108151- Congenital gastrointestinal and neural anomalies
- 3D Printing in Biomedical Research
- Barrier Structure and Function Studies
- Gastrointestinal motility and disorders
- Intestinal Malrotation and Obstruction Disorders
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
- Ocular Surface and Contact Lens
- Salivary Gland Disorders and Functions
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Corneal Surgery and Treatments
- Axon Guidance and Neuronal Signaling
- Congenital heart defects research
- Nanoparticle-Based Drug Delivery
- Diet, Metabolism, and Disease
- Cancer Research and Treatments
- Bacterial Infections and Vaccines
- Drug Solubulity and Delivery Systems
- Digestive system and related health
- Inflammatory Bowel Disease
- Wnt/β-catenin signaling in development and cancer
- Gut microbiota and health
- Pluripotent Stem Cells Research
Fraunhofer Institute for Silicate Research
2018-2025
Universitätsklinikum Würzburg
2014-2024
Fraunhofer Society
2022
Bayer (Germany)
2021
University of Konstanz
2019
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2014-2018
Great Ormond Street Hospital
2016
University College London
2008-2016
Leipzig University
2010-2014
University of Würzburg
2014
In vitro models of the human blood-brain barrier (BBB) are highly desirable for drug development. This study aims to analyze a set ten different BBB culture based on primary cells, induced pluripotent stem cells (hiPSCs), and multipotent fetal neural (fNSCs). We systematically investigated impact astrocytes, pericytes, NSCs hiPSC-derived endothelial cell function gene expression. The quadruple models, these four types, achieved characteristics including transendothelial electrical resistance...
Intestinal in vitro models are valuable tools drug discovery and infection research. Despite several advantages, the standard cell line-based Transwell(®) based for example on colonic epithelial Caco-2 cells, lack cellular complexity transport activity associated with native small intestinal tissue. An additional experimental set-back arises from most commonly used synthetic membranes, which cells routinely cultured. These can lead to an barrier during testing. To overcome these limitations,...
Impaired intestinal epithelial barrier (IEB) function with loss of desmosomal junctional protein desmoglein 2 (DSG2) is a hallmark in the pathogenesis inflammatory bowel disease (IBD). While previous studies have reported that glial cell line–derived neurotrophic factor (GDNF) promotes IEB function, mechanisms are poorly understood. We hypothesized GDNF involved DSG2, resulting impaired as seen IBD. In inflamed intestine patients IBD, there was decrease concentrations accompanied by changes...
Enteric glial cells (EGCs) are the main constituent of enteric nervous system and share similarities with astrocytes from central including their reactivity to an inflammatory microenvironment. Previous studies on EGC pathophysiology have specifically focused mucosal glia activation its contribution processes observed in gut bowel disease (IBD) patients. In contrast knowledge is scarce intestinal inflammation not locally restricted mucosa but systemically affecting intestine effect overall...
Recent data suggest that neurotrophic factors from the enteric nervous system are involved in intestinal epithelial barrier regulation. In this context glial cell line-derived factor (GDNF) was shown to affect gut properties vivo directly or indirectly by largely undefined processes a model of inflammatory bowel disease (IBD). We further investigated potential role and mechanisms GDNF regulation functions. Immunostaining human specimen showed positive staining neuronal plexus enterocytes....
Recent advances in the vitro characterization of human adult enteric neural progenitor cells have opened new possibilities for cell-based therapies gastrointestinal motility disorders. However, whether these are able to integrate within an vivo gut environment is still unclear. In this study, we transplanted progenitor-containing neurosphere-like bodies (NLBs) a mouse model hypoganglionosis and analyzed cellular integration NLB-derived cell types functional improvement. NLBs were propagated...
Irritable bowel syndrome (IBS) is a gut-brain disorder involving alterations in intestinal sensitivity and motility. Serotonin 5-HT4 receptors are promising candidates IBS pathophysiology since they regulate gut motor function stool consistency, targeted 5-HT4R selective drug intervention has been proven beneficial subgroups of patients. We identified single nucleotide polymorphism (SNP) (rs201253747) c.*61 T > C within the receptor gene HTR4 to be predominantly present diarrhoea-IBS...
Meningococcal meningitis is a severe central nervous system infection that occurs when Neisseria meningitidis (Nm) penetrates brain endothelial cells (BECs) of the meningeal blood-cerebrospinal fluid barrier. As human-specific pathogen, in vivo models are greatly limited and pose significant challenge. In vitro cell have been developed, however, most lack critical BEC phenotypes limiting their usefulness. Human BECs generated from induced pluripotent stem (iPSCs) retain properties offer...
Significant advancements in the field of preclinical vitro blood-brain barrier (BBB) models have been achieved recent years, by developing monolayer-based culture systems towards complex multi-cellular assays. The coupling those with other relevant organoid to integrate investigation permeation larger picture drug distribution and metabolization is still missing. Here, we report for first time combination a human induced pluripotent stem cell (hiPSC)-derived model cortical brain liver...
Glioblastoma (GBM) is the most prevalent, malignant, primary brain tumor in adults, characterized by limited treatment options, frequent relapse, and short survival after diagnosis. Until now, none of existing therapy approaches have proven to be an effective cure. The availability predictive human blood-tumor barrier (BTB) test systems that can mimic in-vivo pathophysiology GBM would great interest preclinical research. Here, we present establishment a new BTB in-vitro system combining...
Serum- and glucocorticoid-inducible kinase (SGK1) is transcriptionally upregulated by mineralocorticoids activated insulin. The stimulates the renal epithelial Na + channel may thus participate in blood pressure regulation. Hyperinsulinemia triggered dietary fructose, which sensitizes for salt intake. role of SGK1 hypertensive effects combined fructose high-salt intake was explored knockout mice ( sgk1 −/− ) their wild-type littermates +/+ ). Renal transcript levels were significantly...
In this study, superparamagnetic iron oxide (SPIO) particle-labeled mesenchymal stromal cells (MSCs) were injected intra-articularly into osteoarthritic knee joints. Their fate and distribution evaluated using magnetic resonance imaging (MRI) macroscopic histologic postmortem examination. Osteoarthritis was induced in 12 sheep by bilateral meniscectomy. After 6 weeks, one joint received 10 × 10(6) SPIO-labeled MSCs (Molday Ion Rhodamine B). Contralateral knees a control injection of a) PBS,...
Organoids recapitulate the (patho)physiological processes in certain tissues and organs closer than classical cell lines. Therefore, organoid technology offers great potentials drug development testing, personalized medicine. In particular, organoids can be used to study predict drug-induced toxicity tissues. However, until today few methods had been reported analyze death 3D-microtissues a quantitative manner. Here, we describe novel fluorometric method for measurement of specific death....
The extracellular matrix represents a dynamic microenvironment regulating essential cell functions in vivo. Tissue engineering approaches aim to recreate the native niche vitro using biological scaffolds generated by organ decellularization. So far, specific origin of such was less considered and potential consequences for culture remain largely elusive. Here, we show that cues affect cellular behavior. In detail, report on generation well-preserved pancreatic bioscaffold introduce scoring...
In this report we describe a human pluripotent stem cell-derived vascular progenitor (MesoT) cell of the mesothelium lineage. MesoT cells are multipotent and generate smooth muscle cells, endothelial pericytes self-assemble into vessel-like networks in vitro. transplanted mechanically damaged neonatal mouse heart migrate injured tissue contribute to nascent coronary vessels repair zone. When seeded onto decellularized scaffolds, differentiate major lineages vasculature capable supporting...
The Gram-negative Epsilonproteobacterium Campylobacter jejuni is currently the most prevalent bacterial foodborne pathogen. Like for many other human pathogens, infection studies with C. mainly employ artificial animal or cell culture models that can be limited in their ability to reflect in-vivo environment within host. Here, we report development and application of a three-dimensional (3D) model based on tissue engineering study host-pathogen interactions. Our intestinal 3D built...
Abstract Extensively studied blood–brain barrier (BBB) in‐vitro models are established on 2D cell culture inserts. However, they do not accurately represent 3D in‐vivo microenvironments due to lack of direct neurovascular unit cellular contacts. Here, the establishment and characterization a self‐assembled BBB spheroid model using human‐induced pluripotent stem (hiPSC)‐derived brain capillary endothelial‐like cells (iBCECs) in combination with primary human astrocytes (ACs) pericytes (PCs)...
Lacrimal gland (LG) insufficiency is a main cause for severe dry eye leading to pain, visual impairment, and eventually loss of sight. Engineering transplantable LG tissue with secretory capacity desirable goal. In this study, three-dimensional decellularized (DC-LG) scaffold preserved morphology was generated by treatment 1% sodium deoxycholate DNase solution using porcine tissue. To address clinical applicability, the primary in vitro culture active cells from small biopsy 1.5 mm diameter...
Infection research routinely employs in vitro cell cultures or vivo mouse models as surrogates of human hosts. Differences between murine and immunity the low level complexity traditional cultures, however, highlight demand for alternative that combine -like properties system with straightforward experimental perturbation. Here, we introduce a 3D tissue model comprising multiple types intestinal barrier, primary site pathogen attack. During infection foodborne Salmonella enterica serovar...
Abstract Background The mechanisms underlying loss of intestinal epithelial barrier [IEB] function in Crohn’s disease [CD] are poorly understood. We tested whether human enteroids generated from isolated crypts CD patients serve as an appropriate vitro model to analyse changes IEB proteins observed patients’ specimens. Methods Gut samples and healthy individuals who underwent surgery were collected. Enteroids analyses junctional comparison full wall performed. Results Histopathology...