Ágnes L. Bodor
A5002808282- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Advanced Memory and Neural Computing
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Fetal and Pediatric Neurological Disorders
- Cannabis and Cannabinoid Research
- Animal Vocal Communication and Behavior
- Marine animal studies overview
- Animal Behavior and Reproduction
- Visual perception and processing mechanisms
- Neuroscience and Neural Engineering
- Immune cells in cancer
- Cerebrospinal fluid and hydrocephalus
- Genetic and Kidney Cyst Diseases
- Retinal Development and Disorders
- Medical Image Segmentation Techniques
- Scientific Computing and Data Management
- Advanced Neural Network Applications
- Neuroscience of respiration and sleep
- Embedded Systems Design Techniques
Allen Institute
2015-2024
Allen Institute for Brain Science
2015-2024
Princeton University
2021
Neuroscience Institute
2021
Laboratoire d'Informatique de Paris-Nord
2021
University of Washington
2009-2017
Hungarian Academy of Sciences
2005-2008
Neurons in the developing brain undergo extensive structural refinement as nascent circuits adopt their mature form. This physical transformation of neurons is facilitated by engulfment and degradation axonal branches synapses surrounding glial cells, including microglia astrocytes. However, small size phagocytic organelles complex, highly ramified morphology glia have made it difficult to define contribution these other cell types this crucial process. Here, we used large-scale, serial...
Learning from experience depends at least in part on changes neuronal connections. We present the largest map of connectivity to date between cortical neurons a defined type (layer 2/3 [L2/3] pyramidal cells mouse primary visual cortex), which was enabled by automated analysis serial section electron microscopy images with improved handling image defects (250 × 140 90 μm3 volume). used identify constraints learning algorithms employed cortex. Previous studies modeled continuum synapse sizes...
Mammalian cortex features a vast diversity of neuronal cell types, each with characteristic anatomical, molecular and functional properties. Synaptic connectivity powerfully shapes how type participates in the cortical circuit, but mapping rules at resolution distinct types remains difficult. Here, we used millimeter-scale volumetric electron microscopy 1 to investigate all inhibitory neurons across densely-segmented population 1352 cells spanning layers mouse visual cortex, producing wiring...
Endocannabinoid-mediated retrograde signaling exerts powerful control over synaptic transmission in many brain areas. However, the neocortex, precise laminar, cellular, and subcellular localization of type 1 cannabinoid receptor (CB ) as well its function has been elusive. Here we combined multiple immunolabeling with whole-cell recordings to investigate morpho-functional characteristics rat somatosensory cortex. Immunostaining for CB revealed axonal somatic labeling striking layer...
Inhibitory neurons in mammalian cortex exhibit diverse physiological, morphological, molecular, and connectivity signatures. While considerable work has measured the average of several interneuron classes, there remains a fundamental lack understanding distribution distinct inhibitory cell types with synaptic resolution, how it relates to properties target cells, affects function. Here, we used large-scale electron microscopy functional imaging address these questions for chandelier cells...
Understanding the relationship between circuit connectivity and function is crucial for uncovering how brain implements computation. In mouse primary visual cortex (V1), excitatory neurons with similar response properties are more likely to be synaptically connected, but previous studies have been limited within V1, leaving much unknown about broader rules. this study, we leverage millimeter-scale MICrONS dataset analyze synaptic functional of individual across cortical layers areas. Our...
The scientific mission of the Project MindScope is to understand neocortex, part mammalian brain that gives rise perception, memory, intelligence, and consciousness. We seek quantitatively evaluate hypothesis neocortex a relatively homogeneous tissue, with smaller functional modules perform common computational function replicated across regions. here focus on mouse as model organism genetics, physiology, behavior can be readily studied manipulated in laboratory. describe operation cortical...
Abstract Learning from experience depends at least in part on changes neuronal connections. We present the largest map of connectivity to date between cortical neurons a defined type (L2/3 pyramidal cells), which was enabled by automated analysis serial section electron microscopy images with improved handling image defects. used identify constraints learning algorithms employed cortex. Previous studies modeled continuum synapse sizes (Arellano et al. 2007) log-normal distribution...
Abstract To understand the brain we must relate neurons’ functional responses to circuit architecture that shapes them. Here, present a large connectomics dataset with dense calcium imaging of millimeter scale volume. We recorded activity from approximately 75,000 neurons in primary visual cortex (VISp) and three higher areas (VISrl, VISal VISlm) an awake mouse viewing natural movies synthetic stimuli. The data were co-registered volumetric electron microscopy (EM) reconstruction containing...
Abstract 3D electron microscopy (EM) has been successful at mapping invertebrate nervous systems, but the approach limited to small chunks of mammalian brains. To scale up larger volumes, we have built a computational pipeline for processing petascale image datasets acquired by serial section EM, popular form EM. The employs convolutional nets compute nonsmooth transformations required align images sections containing numerous cracks and folds, detect neuronal boundaries, label voxels as...
A primary cilium is a thin membrane-bound extension off cell surface that contains receptors for perceiving and transmitting signals modulate state activity. While many types have cilium, little known about cilia in the brain, where they are less accessible than on cultured cells or epithelial tissues protrude from bodies into deep, dense network of glial neuronal processes. Here, we investigated frequency, internal structure, shape, position large, high-resolution transmission electron...
Abstract Advances in Electron Microscopy, image segmentation and computational infrastructure have given rise to large-scale richly annotated connectomic datasets which are increasingly shared across communities. To enable collaboration, users need be able concurrently create new annotations correct errors the automated by proofreading. In large datasets, every proofreading edit relabels cell identities of millions voxels thousands like synapses. For analysis, require immediate reproducible...
Giant inhibitory terminals with multiple synapses, the counterparts of excitatory "detonator" or "driver" terminals, have not been described in forebrain. Using three-dimensional reconstructions electron microscopic images, we quantitatively characterize a GABAergic pathway that establishes synaptic contacts exclusively via synapses. Axon nigrothalamic formed, on average, 8.5 synapses large-diameter dendrites and somata relay cells ventromedial nucleus rat thalamus. All given terminal...
Summary We present a semi-automated reconstruction of L2/3 mouse primary visual cortex from 3 million cubic microns electron microscopic images, including pyramidal and inhibitory neurons, astrocytes, microglia, oligodendrocytes precursors, pericytes, vasculature, mitochondria, synapses. Visual responses subset cells are included. The data being made publicly available, along with tools for programmatic 3D interactive access. density synaptic inputs onto neurons varies across cell classes...
Abstract The activity and connectivity of inhibitory cells has a profound impact on the operation neuronal networks. While average many cell types been characterized, we still lack an understanding how individual interneurons distribute their synapses onto targets heterogeneous inhibition is different excitatory neurons. Here, use large-scale volumetric electron microscopy (EM) functional imaging to address this question for chandelier in layer 2/3 mouse visual cortex. Using dense...
A primary cilium is a membrane-bound extension from the cell surface that contains receptors for perceiving and transmitting signals modulate state activity. Primary cilia in brain are less accessible than on cultured cells or epithelial tissues because they protrude into deep, dense network of glial neuronal processes. Here, we investigated frequency, internal structure, shape, position large, high-resolution transmission electron microscopy volumes mouse visual cortex. Cilia extended...
Maps of the nervous system that identify individual cells along with their type, subcellular components and connectivity have potential to elucidate fundamental organizational principles neural circuits. Nanometer-resolution imaging brain tissue provides necessary raw data, but inferring cellular annotation layers is challenging. We present segmentation-guided contrastive learning representations (SegCLR), a self-supervised machine technique produces directly from 3D imagery segmentations....
We are now in the era of millimeter-scale electron microscopy (EM) volumes collected at nanometer resolution (Shapson-Coe et al., 2021; Consortium 2021). Dense reconstruction cellular compartments these EM has been enabled by recent advances Machine Learning (ML) (Lee 2017; Wu Lu Macrina Automated segmentation methods produce exceptionally accurate reconstructions cells, but post-hoc proofreading is still required to generate large connectomes free merge and split errors. The elaborate 3-D...
Neural circuit function is shaped both by the cell types that comprise and connections between those 1 . have previously been defined morphology 2, 3 , electrophysiology 4, 5 transcriptomic expression 6-8 connectivity 9-13 or even a combination of such modalities 14-16 More recently, Patch-seq technique has enabled characterization (M), (E), (T) properties from individual cells 17-20 Using this technique, these were integrated to define 28, inhibitory multimodal, MET-types in mouse primary...
Avian song learning shares striking similarities with human speech acquisition and requires a basal ganglia (BG)–thalamo-cortical circuit. Information processing transmission speed in the BG is thought to be limited by synaptic architecture of two serial inhibitory connections. Propagation may critical avian circuit given temporally precise control musculature during vocalization. We used electrical stimulation cortical inputs study, fine time resolution, functional connectivity within this...
Mammalian neocortex contains a highly diverse set of cell types. These types have been mapped systematically using variety molecular, electrophysiological and morphological approaches. Each modality offers new perspectives on the variation biological processes underlying type specialization. Cellular scale electron microscopy (EM) provides dense ultrastructural examination an unbiased perspective into subcellular organization brain cells, including their synaptic connectivity nanometer...
Diverse sources of GABAergic inhibition are a major feature cortical networks, but distinct inhibitory input systems have not been systematically characterized in the thalamus. Here, we contrasted properties two independent pathways posterior thalamic nucleus rat, one from reticular (nRT), and “extrareticular” anterior pretectal (APT). The vast majority nRT-thalamic terminals formed single synapses per postsynaptic target innervated thin distal dendrites relay cells. In contrast,...
Abstract The anterior pretectal nucleus (APT) and the zona incerta (ZI) are diencephalic nuclei that exert a strong inhibitory influence selectively in higher order thalamic relays. APT is also known to project ZI as well thalamus, but anatomical details of APT‐ZI projection have not been described. In present study, efferent pathways were examined APT‐ZI‐thalamus network by using anterograde retrograde tracing combination with pre‐ postembedding immunocytochemical stainings situ...