A5047088050- Microbial Natural Products and Biosynthesis
- Ethnobotanical and Medicinal Plants Studies
- Fungal Biology and Applications
- Essential Oils and Antimicrobial Activity
- Legume Nitrogen Fixing Symbiosis
- Agronomic Practices and Intercropping Systems
- Phytochemicals and Antioxidant Activities
- Allelopathy and phytotoxic interactions
- Mycorrhizal Fungi and Plant Interactions
- Plant biochemistry and biosynthesis
- Plant nutrient uptake and metabolism
- Biological Control of Invasive Species
- Phytochemistry and Bioactivity Studies
- Chemical synthesis and alkaloids
- Natural Antidiabetic Agents Studies
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Plant Physiology and Cultivation Studies
- Entomopathogenic Microorganisms in Pest Control
- Ecology and Vegetation Dynamics Studies
- Enzyme Catalysis and Immobilization
- Steroid Chemistry and Biochemistry
- Phytoplasmas and Hemiptera pathogens
University of Helsinki
2015-2024
University of Turku
2017-2023
National Institute of Biological Resources
2022
Mountain Medicine Society of Nepal
2020-2022
Pokhara University
2017-2021
Himalayan Rescue Association
2020
Nepal Academy of Science and Technology
1970-2015
Climate change is causing shifts in the habitat, distribution, ecology, and phenology of Himalayan plants. These changes are predicted to continue, jeopardizing survival medicinal plant species local livelihoods that rely on them. We analyzed present future diversity distribution influenced by different climate scenarios, calculated climatic niche using ensemble modeling (eSDM). compiled 1041 (N) geospatial data seven high-value Nepal: Aconitum spicatum (n = 100), Allium wallichii 151),...
Nucleoside antibiotics are a large class of pharmaceutically relevant chemical entities, which exhibit broad spectrum biological activities. Most nucleosides belong to the canonical N-nucleoside family, where heterocyclic unit is connected carbohydrate through carbon–nitrogen bond. However, atypical C-nucleosides were isolated from Streptomyces bacteria over 50 years ago, but molecular basis for formation these metabolites has been unknown. Here, we have sequenced genome S. showdoensis ATCC...
Streptomyces species are prolific sources of bioactive secondary metabolites known especially for their antimicrobial and anticancer activities.This study sought to isolate characterize antioxidant molecules biosynthesized by sp. KTM18. The potential an isolated compound its toxicity were accessed.The was purified using bioassay-guided chromatography techniques. Nuclear magnetic resonance (NMR) experiments carried out structure elucidation. the determined DPPH free radical scavenging assay....
Microbial natural products have been a cornerstone of the pharmaceutical industry, but supply novel bioactive secondary metabolites has diminished due to extensive exploration most easily accessible sources, namely terrestrial Streptomyces species. The Persian Gulf is unique habitat for marine sponges, which contain diverse communities microorganisms including Actinobacteria. These exotic ecosystems may cradle rare actinomycetes with high potential produce metabolites. In this study, we...
Actinomycetes are important producers of pharmaceuticals and industrial enzymes. However, wild type strains require laborious development prior to usage. Here we present a generally applicable reporter-guided metabolic engineering tool based on random mutagenesis, selective pressure, single-cell sorting. We developed fluorescence-activated cell sorting (FACS) methodology capable reproducibly identifying high-performing individual cells from mutant population directly liquid cultures. an...
Acid lime (Citrus aurantifolia (Christm.) Swingle) is an important fruit crop, which has high commercial value and cultivated in 60 out of the 77 districts representing all geographical landscapes Nepal. A lack improved high-yielding varieties, infestation with various diseases, pests, as well poor management practices might have contributed to its extremely reduced productivity, necessitates a reliable understanding genetic diversity existing cultivars. Hereby, we aim characterize acid...
Plants have a pivotal role in ethnopharmacology, and their preparations are use globally. However, getting down to the structure requires an effective workflow mostly time-consuming isolation process. Although bioassay-guided approaches widely popular, they face massive problem of rediscovery recent times, especially plant metabolomics. Mass spectrometry (MS)-based approach incorporated molecular networking via Global Natural Product Social Molecular Networking (GNPS) is considered here for...
Antioxidant capacities and phenolic contents of medicinal plants namely Usnea longifolia, Cetraria nepalensis, Parmelia minarum, Everniastrum nepalense, Rhododendron anthopogon Fritillaria delavayi were analyzed via Folin-Ciocaltau assay, Ferric reducing activity power assay 2,2-Diphenyl-1-picrylhydrazyl assay. All the tested depicted antioxidant with variation in extent among plants. The FRAP (F-value: 387.4), DPPH 89.684) TPC 559.163) values between extracts showed highly significant...