A5076994622- Microbial Natural Products and Biosynthesis
- Phytoestrogen effects and research
- Diet, Metabolism, and Disease
- Genomics and Phylogenetic Studies
- Fatty Acid Research and Health
- Fungal Biology and Applications
- Phytochemicals and Antioxidant Activities
- Regulation of Appetite and Obesity
- Monoclonal and Polyclonal Antibodies Research
- Garlic and Onion Studies
- Malaria Research and Control
- Mycotoxins in Agriculture and Food
- Gut microbiota and health
- Invertebrate Immune Response Mechanisms
- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Diet and metabolism studies
- Nutrition, Genetics, and Disease
- SARS-CoV-2 and COVID-19 Research
- Chemical Synthesis and Analysis
- Protein Hydrolysis and Bioactive Peptides
- Diverse Scientific Research Studies
- Muscle Physiology and Disorders
- Glycosylation and Glycoproteins Research
- Marine Sponges and Natural Products
University of Tübingen
2020-2023
Ehime University
2015-2022
Kathmandu University
1970
Lasso peptides are a class of ribosomally synthesized and post-translationally modified (RiPPs) that feature an isopeptide bond distinct lariat fold. A growing number secondary modifications have been described further decorate lasso peptide scaffolds. Using genome mining, we discovered pair biosynthetic gene clusters (BGCs) include cytochrome P450 genes. mass spectrometry, stable isotope incorporation, extensive 2D-NMR report the structural characterization two unique examples (C-N)...
Streptomyces species are prolific sources of bioactive secondary metabolites known especially for their antimicrobial and anticancer activities.This study sought to isolate characterize antioxidant molecules biosynthesized by sp. KTM18. The potential an isolated compound its toxicity were accessed.The was purified using bioassay-guided chromatography techniques. Nuclear magnetic resonance (NMR) experiments carried out structure elucidation. the determined DPPH free radical scavenging assay....
Actinomycetes are natural architects of numerous secondary metabolites including antibiotics. With increased multidrug-resistant (MDR) pathogens, antibiotics that can combat such pathogens urgently required to improve the health care system globally. The characterization actinomycetes available in Nepal is still very much untouched which reason why this paper showcases from based on their morphology, 16S rRNA gene sequencing, and metabolic profiling. Additionally, antimicrobial assays liquid...
Plants have a pivotal role in ethnopharmacology, and their preparations are use globally. However, getting down to the structure requires an effective workflow mostly time-consuming isolation process. Although bioassay-guided approaches widely popular, they face massive problem of rediscovery recent times, especially plant metabolomics. Mass spectrometry (MS)-based approach incorporated molecular networking via Global Natural Product Social Molecular Networking (GNPS) is considered here for...
We compared the effects of two major isoflavones, daidzein and genistein, on lipid metabolism in rats. Daidzein (150 mg/kg diet), genistein (1:1, 300 or control diets were fed to 4 groups 6-week-old ovariectomized (Ovx) non-Ovx Sprague Dawley rats for weeks. Dietary daidzein, but not reduced serum hepatic total cholesterol levels significantly relative that by group, regardless whether had undergone ovariectomy. Genistein did exhibit any physiological levels, affect genes involved...
A chemical reinvestigation of the Indonesian strain Streptomyces sp. SHP 22-7 led to isolation three new pyrimidine nucleosides, along with six known analogues and zincphyrin. The structures compounds (6, 7, 10) were elucidated by employing spectroscopic techniques (NMR, MS, CD, IR) as well enantioselective analyses methyl branched side chain configurations. Application precursor-directed feeding approach production partial nine further analogues. 6, 11 (2–4) found possess antimycobacterial...
We investigated the effect of daidzein feeding and estradiol treatment on food intake in cholecystokinin-1 receptor (CCK1R) deficiency, leptin (ObRb) deficiency rats their wild-type rats. These underwent an ovariectomy or a sham operation. For 5 week experiment, each rat was divided three groups: control, (150 mg/kg diet), (4.2 μg/rat/day) groups. In both CCK1R+ CCK1R- rats, significantly decreased intake. Daidzein reduced ovariectomized ObRb- although not ObRb+ Estradiol lowered increases...
Two new ircinianin-type sesterterpenoids, ircinianin lactone B and C (7 8), together with five known entities from the compound family (1, 3-6) were isolated marine sponge Ircinia wistarii. Ircinianin lactones 8) represent terpenoids a modified oxidation pattern. Despite their labile nature, structures could be established using combination of spectroscopic data, including HRESIMS 1D/2D NMR techniques, as well computational chemistry quantum-mechanical calculations. In broad screening...
Crude chloroform/methanol and methanol extracts of leaves twigs three medicinal plants collected from different altitudes Nepal, Rhododendron lepidotum, Hippophae rhamnoids Cornus capitata were examined for their antibacterial, cytotoxicity antiproliferative activities. The antimicrobial activity shown by R. lepidotum C. against Staphylococcus aureus was comparable with that streptomycin antibiotic whereas the extract H. very low. Cytoxicity (LC50) values ranged 46.4 to 111.6 μg/ml 129.4...
Abstract Lasso peptides are a class of ribosomally synthesized and post-translationally modified (RiPPs) that feature an isopeptide bond distinct lariat fold. A growing number secondary modifications have been described further decorate lasso peptide scaffolds. Using genome mining, we discovered pair biosynthetic gene clusters (BGCs) include cytochrome P450 genes. Here, report the structural characterization two unique examples (C-N) biaryl-containing peptides. Nocapeptin A, from Nocardia...
We previously found that daidzein decreased food intake in female rats. The present study aimed to elucidate the relationship between dynamics of appetite-mediated neuropeptides and anorectic effect daidzein. examined gene expression hypothalamus small intestine during 3 meals per day feeding method. Daidzein had an specifically at second feeding. Neuropeptide-Y (NPY) galanin mRNA levels were significantly higher after control but not group, suggesting attenuated postprandial increase NPY...
The promotion of muscle recovery after immobilization is important to preserve an optimum health status. Here, we examined the effect dietary Alaska pollack protein (APP) on skeletal weight atrophy induced by hind limb using plaster technique. Rat left was casted with a wetted cast under anesthesia. After 2 weeks feeding, removed and rats were divided into three groups, namely, baseline group, high-fat casein diet APP group. 3 muscles (soleus, extensor digitorum longus [EDL], gastrocnemius)...
We previously found that daidzein decreased food intake in female rats. To understand the mechanism of anorectic action dietary daidzein, it is necessary to determine distributions and S-equol, a metabolite intestinal bacterial conversion from body. In present study, we measured concentrations S-equol serum bile sham-operated ovariectomized rats fed diet containing 150 mg/kg for 7 days. Dietary increased far higher levels than those daidzein. concentration was more several hundred...
AbstractCoronavirus disease-19 (COVID-19) pandemic is a global threat caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The viral infection dysregulates the functions of renin-angiotensin system (RAS) through an interaction between SARS-CoV-2 spike protein and angiotensin-converting enzyme 2 (ACE2), leading to upregulated level Angiotensin II (Ang II) in blood plasma. enhanced Ang may contribute various pathophysiological events, including vasoconstriction, oxidative...
Jimbu (Allium przewalskianum), is an indigenous Himalayan spice herb in Nepal and has a long history of culinary use, yet its potential beyond home kitchens remains largely unexplored due to limited studies exposure. It does not require extensive input cash, machinery, land, or labor profit from, making it excellent valuable source pharmaceutical ingredients owing bioactive compounds. We investigated antioxidant antimicrobial properties. Quantitative analysis extracts revealed the presence...
ABSTRACT We previously found that equol, a metabolite of intestinal bacterial conversion from soy isoflavone daidzein, has female-specific anorectic effects. In the present study, we used seven-week-old female ovariectomized (OVX) Sprague Dawley rats to test hypothesis effect dietary daidzein may be attributed delayed gastric emptying. Results suggest delays emptying and it an with residual contents, but not without contents. Dietary equol significantly decreased daily food intake in OVX...
Novel Techniques in Nutrition and Food Science The Food-Borne Micro RNA its Controversy on Human Health Keshab Bhattarai* Sudhashree Adhikari Nutritional Laboratory, United Graduate School of Agricultural Sciences, Ehime University, Japan *Corresponding author:Keshab Bhattarai, Agriculture, 3-5-7 Tarumi, Matsuyama, 790-8566, Submission: April 19, 2018;Published: May 31, 2018 DOI: 10.31031/NTNF.2018.02.000533 ISSN:2640-9208Volume2 Issue2