A5023456669- Metabolism, Diabetes, and Cancer
- Barrier Structure and Function Studies
- Cell death mechanisms and regulation
- Connexins and lens biology
- Wound Healing and Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- Cell Adhesion Molecules Research
- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Dermatologic Treatments and Research
- Cancer, Lipids, and Metabolism
- Carcinogens and Genotoxicity Assessment
- Adenosine and Purinergic Signaling
- Acute Ischemic Stroke Management
- Lipid metabolism and biosynthesis
- Amino Acid Enzymes and Metabolism
- Nuclear Structure and Function
- Cholesterol and Lipid Metabolism
- Caveolin-1 and cellular processes
- Bone health and treatments
- Bone health and osteoporosis research
Fukuoka Dental College
2018-2024
Nagoya City University
2023
Kyushu University
2015
Kyushu Dental University
2013
Abstract The uncarboxylated form of osteocalcin (GluOC) regulates glucose and lipid metabolism in mice. We previously showed that low-dose (≤10 ng/ml) GluOC induces the expression adiponectin peroxisome proliferator-activated receptor γ (PPARγ) via a cAMP–PKA–ERK–CREB signaling pathway 3T3-L1 adipocytes. also noticed high-dose (≥20 inhibits PPARγ these cells. have here explored mechanism underlying effects GluOC. High-dose triggered morphological changes adipocytes suggestive induction cell...
Nisin is a food preservative produced by Lactococcus lactis subsp. lactis. Previous blood biochemical research revealed that nisin has physiological effects in mammals; although the site of action yet to be identified, keratinocytes have been proposed as possible target. In this study, we investigated whether affects examining on eukaryotic intermediate filaments HaCaT human keratinocytes. Treatment with 93 μg/ml for 24 h decreased localization filament proteins cytokeratin (CK)5 and CK17 at...
The G protein–coupled receptor GPRC6A regulates various physiological processes in response to its interaction with multiple ligands, such as extracellular basic amino acids, divalent cations, testosterone, and the uncarboxylated form of osteocalcin (GluOC). Global ablation increases susceptibility mice diet-induced obesity related metabolic disorders. However, given that is expressed many tissues responds a variety hormonal nutritional signals, cellular molecular mechanisms underlying...
Osteocalcin is a bone-derived hormone that in its uncarboxylated form (GluOC) plays an important role glucose and energy metabolism by stimulating insulin secretion pancreatic β-cell proliferation through putative receptor GPRC6A. We previously showed the effect of GluOC on mediated predominantly glucagon-like peptide-1 (GLP-1) released from intestinal endocrine cells response to stimulation. Moreover, oral administration was found reduce fasting blood level, improve tolerance, increase...
Intestinal epithelial cells are the first targets of ingested mycotoxins, such as ochratoxin A, citrinin and deoxynivalenol. It has been reported that paracellular permeability regulated by tight junctions is modulated several mycotoxins reducing expression specific claudins integral membrane proteins in cell–cell contacts, accompanied increase phosphorylation mitogen-activated protein kinases, including extracellular signal-related kinase (ERK) 1/2, p38 c-Jun NH2-terminal kinase. Claudin-2...
Abstract Retinoic acid (RA) plays an important role in epithelial homeostasis and influences the morphology, proliferation, differentiation permeability of cells. Mouse keratinocytes, K38, reconstituted non-keratinized stratified epithelium three-dimensional (3D) cultures with serum, which contains retinol (a source RA), but morphology was different from vivo epithelium. The formed thick, loosened cell–cell contacts. Here, we investigated whether inhibition RA receptor (RAR)/retinoid X...
Abstract BACKGROUNND We investigated the characteristic variables of acute ischemic stroke categorized according to body mass index (BMI). METHODS From a registry 1676 consecutive cases, 965 cases (622 men and 434 women; mean age 71.53 ± 12.42 years) were selected based on eligibility criteria. These participants divided into four cohorts BMI classifications: < 18 (underweight), 18.5 ≤ 25 (normal weight), 30 (overweight), ≥ (obese). To avoid bias, propensity score analyses performed;...
Phospholipase C (PLC)‐related but catalytically inactive protein (PRIP) is a with domain organization similar to PLC‐δ1. We have reported that PRIP interacts the catalytic subunits of phosphatases, PP1c and PP2Ac, depending on phosphorylation level PRIP. also found Akt was precipitated along by anti‐PRIP antibody from neuronal cells. In this study, we investigated interaction between Akt. Co‐immunoprecipitation assay performed using COS7 cells expressing glutathione S‐transferase (GST)‐fused...
Insulin stimulates glucose uptake into skeletal muscle and adipose tissues by increasing the available number of transporter (GLUT) 4 on cell surface. GLUT4‐loaded vesicles are targeted to plasma membrane from intracellular reservoir through multiple trafficking steps that regulated protein kinase Akt. Although many clues regulate targeting docking GLUT4‐vesicles had been identified, it is still largely unknown how fusion promoted insulin. Thus, we tried identify a new target Akt which...
An acidic environment in bone is essential for metabolism and the production of decarboxylated osteocalcin, which functions as a regulatory hormone glucose metabolism. Here, we describe high-resolution X-ray crystal structure osteocalcin under conditions. Decarboxylated at pH 2.0 retains α-helix native with three γ-carboxyglutamic acid residues neutral pH. This implies that stable an bone. In addition, site-directed mutagenesis revealed Glu17 Glu21 are important adiponectin-inducing activity...
Osteocalcin, one of non-collagenous bone matrix proteins, is known to regulate the energy metabolism in uncarboxylated glutamate form designated as GluOC. We examined effects GluOC using 3T3-L1 adipocytes, and revealed signaling pathway by which ranging 1 ng/ml 10 increased expressions adiponectin, an adipokine regulating glucose lipids, peroxisome proliferator-activated receptor-gamma (PPAR-gamma), a master regulator adipogenesis, via its putative receptor GPRC6A. By contrast, we also found...