A5102884155- Cancer Cells and Metastasis
- Genetic factors in colorectal cancer
- Yersinia bacterium, plague, ectoparasites research
- Cancer-related molecular mechanisms research
- Pharmacological Effects of Natural Compounds
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Helicobacter pylori-related gastroenterology studies
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Colorectal Cancer Treatments and Studies
- RNA modifications and cancer
- Zoonotic diseases and public health
- Gastroesophageal reflux and treatments
- Gastric Cancer Management and Outcomes
- Eosinophilic Esophagitis
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Thyroid and Parathyroid Surgery
- Pediatric Pain Management Techniques
- Digestive system and related health
- DNA Repair Mechanisms
- Anesthesia and Pain Management
Southern Medical University
2011-2024
Nanfang Hospital
2011-2024
Western University
2022-2023
Genomics (United Kingdom)
2014
In this study, we explored the role of Hedgehog signaling pathway in liver regeneration.Retrorsine, partial hepatectomy and combined were utilized mature Fisher344 rats. Specific assays, such as RT-PCR, real-time PCR immunohistochemistry used to detect constituents during regeneration.mRNA expression Ihh, Ptc, Smo, Gli1, Gli2 Gli3 all shown tissue homogenates control/normal, retrorsine, retrorsine/partial groups while no Shh was found. Real-time analysis indicated that there significant...
Abstract Sex-determining region Y-box2 (SOX2), a master regulator of embryonic and induced pluripotent stem cells, drives cancer cells (CSCs) properties, fuels tumor initiation, contributes to aggressiveness. Our previous study has demonstrated the oncogenic role SOX2 in colorectal (CRC). In this study, we sought elucidate underlying mechanisms. Cell function experiments were performed detect chemoresistance, proliferation, stemness, migration, invasion vitro. Chromatin immunoprecipitation,...
Metabolic remodeling is a strategy for tumor survival under stress. However, the molecular mechanisms during metabolic of colorectal cancer (CRC) remain unclear. Melanocyte proliferating gene 1 (MYG1) 3'-5' RNA exonuclease and plays key role in mitochondrial functions. Here, we uncover that MYG1 expression upregulated CRC progression highly expressed promotes glycolysis independent its activity. Mechanistically, nuclear recruits HSP90/GSK3β complex to promote PKM2 phosphorylation, increasing...
Abstract Growing evidence indicates that a small number of cancer cells express stem cell markers and possess cell-like properties promote malignant progression. Sex-determining region Y-box2 (SOX2) is transcription factor essential for maintaining the (CSC). As CSC have been associated with angiogenesis vasculogenic mimicry (VM), we aimed to comprehensively investigate whether SOX2 regulates properties, angiogenesis, VM in colorectal carcinoma (CRC) its potential mechanism this study. For...
Fas signaling has been shown to induce the epithelial-mesenchymal transition (EMT) promote gastrointestinal (GI) cancer metastasis, but involvement of microRNA in this mechanism remains unknown. We found that ligand (FasL) treatment inhibited E-cadherin expression and promoted cell invasion by upregulation miR-23a, overexpression miR-23a inhibitor could partially block activity. FasL-induced extracellular signal-regulated kinase/mitogen-activated protein kinase activated activator 1 (AP-1)...
Abstract Vasculogenic mimicry (VM), a new model of angiogenesis, fulfills the metabolic demands solid tumors and contributes to tumor aggressiveness. Our previous study demonstrated effect SOX2 in promoting VM colorectal cancer (CRC). However, underlying mechanisms behind this remain elusive. Here, we show that overexpression enhanced glycolysis sustained formation via transcriptional activation lncRNA AC005392.2. Suppression either or AC005392.2 expression curbed SOX2-driven vivo vitro....
Peutz–Jeghers syndrome (PJS) is a rare hereditary disorder resulting from mutations in serine/threonine kinase 11 (STK11) and characterized by gastrointestinal (GI) hamartomatous polyps, mucocutaneous pigmentation, an increased risk for specific cancers. Little known about the genetic implications of STK11 with regard to their role dysplastic malignant transformation GI polyps. Peripheral blood genomic DNA samples 116 Chinese PJS patients 52 unrelated families were investigated mutations....
The aim of this study was to compare chromoendoscopy (CE), narrow band imaging (NBI), and confocal laser endomicroscopy (CLE) in diagnosing atrophic gastritis.Atrophic gastritis, especially metaplastic atrophy, has been shown be a risk factor for gastric cancer. Some advanced endoscopic techniques have used diagnose gastritis. However, it is still difficult atrophy with high degree accuracy.In total, 253 sites from 87 consecutive patients were examined by NBI, CE, CLE, turn diagnoses made....
In this study, we aimed to investigate the distribution of virulence genes in clinical isolates pathogenic Yersinia enterocolitica. Two thousand six hundred stool samples were collected from 2600 patients with diarrhea, and tested using culture method real-time PCR. Then, all Y. enterocolitica cultured examined for (inv, ail, ystA, ystB, ystC, yadA, virF) by PCR presence plasmid four phenotypic tests. As a result, 160 strains successfully detected method, including bio/serotype 1A/unknown...
// Haoxuan Zheng 1, * , Zhizhong Liu 2, Tao Yidong Cai 3, Yadong Wang 1 Shiyong Lin 4 Jinmin Chen Jing Zhiqing Bo Jiang Guangdong Provincial Key Laboratory of Gastroenterology, Department Nanfang Hospital, Southern Medical University, Guangzhou 510515, China 2 the Second People's Hospital Zhuhai, Zhuhai 519020, 3 Xiyuan Academy Chinese Sciences, Beijing 100091, Endoscopy and Laser, Sun Yat-sen University Cancer Center; State Oncology in South China; Collaborative Innovation Center for...
Fas signaling promotes colorectal cancer (CRC) metastasis by inducing epithelial‐mesenchymal transition (EMT). The acquisition of EMT properties in turn induces stemness but the mechanism which contributes to it still remains unclear. Hence, aim this study was investigate how regulates CRC stemness. For purpose, soft agar assay, sphere formation cell survival analysis, immunoblot, qRT‐PCR, chromatin immunoprecipitation, and luciferase reporter assay were performed. Expression FasL, Bmi1,...
To establish and evaluate a quick accurate DNA microarray method to detect intestinal pathogens directly from human diarrheal stool samples as an alternative traditional culture methods.Primers 21 oligonucleotide probes based on sequences of the bacterial 16SrRNA gene were arrayed slides. Hybridization between amplicons was performed. determine consistency method, 1,500 clinical 200 normal healthy individuals examined in double-blind fashion. Basic information patients collected analyzed.Our...
Our purpose was to establish a quick and accurate real-time PCR (rtPCR) method detect Campylobacter jejuni directly from human diarrheal stool as an alternative traditional culture methods. To determine the consistency of rtPCR method, 256 clinical samples 50 normal healthy individuals were examined, whole process double-blinded. data showed that sensitivity in pure cultures 10 2 CFU·mL –1 3 CFU·g , respectively. Of samples, specimens successfully detected by both methods, whereas two...
Objective: To carry out a preliminary epidemiological investigation of Yersinia enterocolitica from clinical perspective in China and to further validate the consistency real-time polymerase chain reaction (RT-PCR) culture method. Methods: 700 stool samples were randomly continuously collected in- outpatients with diarrhea, which detected by both method RT-PCR. Results: All 52 positive successfully RT-PCR The total incidence yersiniosis was about 7.4%, statistically higher than before...
Abstract Background Peutz-Jeghers syndrome (PJS) is caused by mutations in the tumor suppressor gene, STK11, and characterized gastrointestinal hamartomas, melanin spots on lips, an increased risk of developing cancer. Methods Blood samples were collected from two unrelated Chinese PJS families totaling 20 individuals (9 male 11 females), including 6 patients. The entire coding region STK11 gene was amplified polymerase chain reaction analyzed direct sequencing. Results A novel mutation,...
Peutz‑Jeghers syndrome (PJS) is a rare hereditary disease caused by mutations in serine threonine kinase 11 (STK11) and characterized an increased risk of developing cancer. Inactivation STK11 has been associated with the mammalian target rapamycin (mTOR) pathway. Hyperactivation phosphorylation key downstream genes ribosomal protein S6 1 (S6K1) promote synthesis cell proliferation. To better understand effects dysfunction pathogenesis PJS, genomic DNA samples from 21 patients PJS unrelated...