A5048258337- Photochromic and Fluorescence Chemistry
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Luminescence and Fluorescent Materials
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Luminescence Properties of Advanced Materials
- Plant Pathogenic Bacteria Studies
- Supramolecular Self-Assembly in Materials
- Legume Nitrogen Fixing Symbiosis
- Lipid Membrane Structure and Behavior
- Molecular Sensors and Ion Detection
- Porphyrin and Phthalocyanine Chemistry
- Alzheimer's disease research and treatments
- Ubiquitin and proteasome pathways
- Head and Neck Surgical Oncology
- Biotin and Related Studies
- Antibiotic Resistance in Bacteria
- Spectroscopy and Quantum Chemical Studies
- Prion Diseases and Protein Misfolding
- Amyotrophic Lateral Sclerosis Research
Institute of Chemistry, Academia Sinica
2015-2025
Academia Sinica
2013-2023
Institute of Chemistry
2017
Stony Brook School
2016
Stony Brook University
2016
Central Taiwan University of Science and Technology
2011-2016
Institute of Biological Chemistry, Academia Sinica
2016
National Tsing Hua University
2008-2016
Ming Chi University of Technology
2011-2014
Korea University
2012-2013
We describe the design of fluorescent, thermoresponsive microgels surface-functionalized with folic acid. Incubation these particles KB cells grown in folate-free medium results efficient endocytosis via a receptor-mediated pathway. Laser scanning confocal microscopy and flow cytometry show uptake folate-modified over cationic control particles. Staining Lysotracker red, followed by imaging, shows anticorrelation between particle endosome fluorescence, which is taken as evidence escape from...
Production of multicolor or multiple wavelength lasers over the full visible-color spectrum from a single chip device has widespread applications, such as superbright solid-state lighting, color laser displays, light-based version Wi-Fi (Li-Fi), and bioimaging, etc. However, designing lasing devices remains challenging issue owing to material requirements for producing emissions sophisticated design action. Here we demonstrate simple highly efficient segment white random based on...
Nasal polyposis is a multi-factorial disease associated with chronic inflammatory condition of the paranasal sinuses. Myofibroblast differentiation and extracellular matrix (ECM) accumulation are involved in pathogenesis nasal polyposis.The aim this study was to effect trichostatin A (TSA), histone deacetylase (HDAC) inhibitor, on transforming growth factor (TGF)-β1-induced myofibroblast ECM polyp-derived fibroblasts (NPDFs).Nasal were isolated from polyps patients who have rhinosinusitis...
Three near-infrared ratiometric fluorescent probes with different p<italic>K</italic><sub>a</sub> values were synthesized to achieve sensitive visualization of pH variations in lysosomes.
Background and Objective Periodontal disease is the most common chronic inflammatory known to mankind (and major cause of tooth loss in adult population) has also been linked various systemic diseases, particularly diabetes mellitus. Based on literature linking periodontal with a “bidirectional manner”, objectives current study were determine: (i) effect model periodontitis, complicated by diabetes, mechanisms tissue breakdown including bone loss; (ii) response combination this local...
Two water-soluble near-infrared luminescent probes, which possess both conventional intense Stokes fluorescence and unique single-photon frequency upconversion luminescence (FUCL), were developed for sensitive selective detection of pH changes in live cells. The water solubility biocompatibility these probes achieved by introducing mannose residues through 2,2'-(ethylenedioxy)diethylamine tethered spacers to a (CF) FUCL organic fluorophore. At higher than 7.4, the have ring-closed...
Aptamer-based nanovectors that actively bind to circulating monocytes enhance drug delivery the heart and cancer.
Two near-infrared luminescent probes with Stokes-shift and single-photon anti-Stokes-shift fluorescence properties for sensitive determination of pH variance in lysosomes have been synthesized. A morpholine residue probe which serves as a targeting group viable cells was attached to the fluorophores via spirolactam moiety while mannose ligated B resulting increased biocompatibility solubility water. Probes contain closed moieties, show no or under neutral alkali conditions. However,...
Photodynamic therapy (PDT) is a noninvasive medical technology that has been applied in cancer treatment where it accessible by direct or endoscope-assisted light irradiation. To lower phototoxicity and increase tissue penetration depth of light, great effort focused on developing new sensitizers can utilize red near-infrared (NIR) for the past decades. Lanthanide-doped upconversion nanoparticles (UCNPs) have unique property to transduce NIR excitation UV–vis emission efficiently. This...
Containing an ensemble of nitrogen-vacancy centers in crystal matrices, fluorescent nanodiamonds (FNDs) are a new type photostable markers that have found wide applications light microscopy. The nanomaterial also has dense carbon core, making it visible to electron Here, we show FNDs encapsulated biotinylated lipids (bLs) useful for subdiffraction imaging antigens on cell surface with correlative light-electron microscopy (CLEM). lipid encapsulation enables not only good dispersion the...
Photoactivatable (caged) bioeffectors provide a way to remotely trigger or disable biochemical pathways in living organisms at desired time and location with pulse of light (uncaging), but the phototoxicity ultraviolet (UV) often limits its application. In this study, we have demonstrated near-infrared (NIR) photoactivatable enzyme platform using protein kinase A (PKA), an important cell biology. We successfully photoactivated PKA NIR phosphorylate substrate, induced downstream cellular...
We demonstrate the targeted optogenetic activation of ChR2 in living cells<italic>via</italic>upconversion nanoparticles with a low power near-infrared illumination.
The amyloidogenic core in the TAR DNA-binding protein (TDP-43) C-terminal fragment has been characterized with its chemical, biochemical, and structural properties delineated. Various of sequence, including membrane impairment ability seeding effect, have also studied.
A strategy for the construction of a profluorescent caged enzyme is described. An active site-directed peptide-based affinity label was designed, synthesized, and employed to covalently nonactive site residue in cAMP-dependent protein kinase. The modified kinase displays minimal catalytic activity low fluorescence. Photolysis results partial cleavage enzyme-bound label, restoration enzymatic (60−80%) strong fluorescent response (10−20 fold). analogous behavior living cells, inducing...
Falling apart, on cue: Signaling pathways often display a profound spatiotemporal component that is best studied using light-activatable reagents. Three separate photolabile moieties can be distinguished based upon their response to three distinct wavelengths (360, 440, and 560 nm) have been synthesized evaluated. This tri-color system also applied imaging in microwells HeLa cells (see picture). Detailed facts of importance specialist readers are published as "Supporting Information". Such...
Abstract Cells identify defective mitochondria and eliminate them through mitophagy: this allows cells to rid themselves of unwanted stress maintain health avoid the activation cell death. One approach experimentally investigate mitophagy is use mitochondrial photosensitizers, which when coupled with light one precisely control damage spatial temporal precision. Here we report three far-red fluorophores that can be used as robust photosensitizers initiate mitophagy. The dyes offer maximal...
ABSTRACT An xps gene cluster composed of 11 open reading frames is required for the type II protein secretion in Xanthomonas campestris pv. campestris. Immediately upstream xpsD gene, which encodes an outer membrane that serves as channel by forming multimers, there exists frame (previously designated ORF2) could encode a 261 amino acid residues. Its N-terminal hydrophobic region likely membrane-anchoring sequence. Antibody raised against this detect wild-type strain X. band with apparent...
Summary Background Nasal polyposis is characterized by tissue remodelling and oedematous nasal mucosa. Vascular endothelial growth factor ( VEGF ) plays a significant role in the regulation of polyps. TLR 4 activation associated with expression murine macrophages odontoblasts. Objective This study aimed to evaluate whether lipopolysaccharide LPS ), an inducer 4, stimulates determine mechanism underlying production Methods polyp‐derived fibroblasts NPDFs were isolated from 10 patients polyps...
Emergence of multidrug-resistant Acinetobacter baumannii (MDRAB) has become a critical clinical problem worldwide and limited therapeutic options for infectious diseases caused by MDRAB. Therefore, there is an urgent need the development new antimicrobial agents or alternative therapy to combat MDRAB infection. The aim this study was investigate effects Mastoparan-AF (MP-AF), amphipathic peptide isolated from hornet venom Vespa affinis with broad-spectrum activity, on As compared used...
The proteasome, a multicatalytic protease, displays distinct chymotrypsin-like, caspase-like, and trypsin-like activities at three different subunits of the multimeric complex. Fluorescent substrates for each these active sites have been described. However, since fluorescent properties are very similar, it is not possible to simultaneously monitor catalysis two or more activities. We developed long wavelength (λex = 600 nm, λem 700 nm) substrate chymotrypsin-like site via combinatorial...
Light-regulatable compounds are finding increasing utility as spatial and temporal probes of biological behavior. An independent measure successful light-induced structural change is possible when alteration (e.g., activation, deactivation, etc.) the bioprobe can be directly linked to a fluorescent readout. We have identified series photolabile fluorescently quenched cassettes that display extraordinarily large fluorescence enhancements upon photolysis. A pair has been inserted into...