A5082160574- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
- Plant Virus Research Studies
- Garlic and Onion Studies
- Cell Image Analysis Techniques
- Plant and animal studies
- Plant tissue culture and regeneration
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Species Descriptions
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Polyamine Metabolism and Applications
- Biochemical and Molecular Research
- Plant Disease Resistance and Genetics
- Metalloenzymes and iron-sulfur proteins
- Insect symbiosis and bacterial influences
- Plant Pathogenic Bacteria Studies
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- ATP Synthase and ATPases Research
BGI Group (China)
2020-2024
BGI Research
2023-2024
Zhejiang University of Technology
2022
China Agricultural University
2014-2017
Understanding the complex functions of plant leaves requires a thorough characterization discrete cell features. Although single-cell gene expression profiling technologies have been developed, their application in characterizing subtypes has not achieved yet. Here, we present scStereo-seq (single-cell spatial enhanced resolution omics sequencing) that enabled us to show bona fide transcriptome profiles Arabidopsis leaves. Subtle but significant transcriptomic differences between upper and...
Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation crop engineering. Pluripotent callus cells develop into fertile shoots through shoot regeneration. The molecular basis of the process remains largely elusive. This study pioneers exploration spatial transcriptome tomato during findings reveal presence highly heterogeneous populations within callus, including epidermis, vascular tissue, primordia, inner outgrowth shoots. By characterizing spatially...
Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-specific phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering intracellular Ca2+ concentration. However, individual members PLCs involved this process need to be identified. Here, function AtPLC4 response Arabidopsis seedlings was analysed. plc4 mutant showed hyposensitivity compared with Col-0 wild-type seedlings, hyposensitive phenotype...
Abstract Callus is a reprogrammed transitional cell mass during plant regeneration. Pluripotent callus cells develop into fertile shoots through de novo shoot organogenesis. This study represents pioneering effort in exploring the spatial transcriptome of tomato regeneration, using technologies including BGI Stereo-seq, BMKMANU S1000, and 10x Visium. The results indicate that comprises highly heterogeneous cells, classified various types based on gene expression histological observation,...
Summary Understanding the complex functions of plant leaves requires spatially resolved gene expression profiling with single-cell resolution. However, although in situ technologies have been developed, this goal has not yet achieved. Here, we present first transcriptome plant, scStereo-seq (single-cell SpaTial Enhanced REsolution Omics-sequencing), which enabled bona fide spatial Arabidopsis leaves. We successfully characterized subtle but significant transcriptomic differences between...
Abstract De novo shoot regeneration from a callus plays crucial role in both plant biotechnology and the fundamental research of cell totipotency. Recent studies have revealed many regulatory factors involved this developmental process. However. our knowledge heterogeneity fate transition during de is still limited. Here, we performed time-series single-cell transcriptome experiments to reveal redifferentiation trajectories early stage regeneration. Based on data 35,669 cells at five-time...
Clean and sustainable H2 production is crucial to a carbon-neutral world. generation by Chlamydomonas reinhardtii an attractive approach for solar-H2 from H2O. However, it currently not large-scalable because of lacking desirable strains with both optimal productivity sufficient knowledge underlying molecular mechanism. We hereby carried out extensive in-depth investigations photoproduction hpm91 mutant PGR5 (Proton Gradient Regulation 5) toward its up-scaling fundamental mechanism issues....
In the present study, we reported and characterized complete chloroplast genome of a moth orchid, Phalaenopsis wilsonii, which is endemic to South China. Its plastid size 145,373 bp, consisting large single copy (LSC) region (84,996 bp), small single-copy (10,668 two inverted repeats (IRs) regions (24,855 bp). A total 122 genes were annotated, comprising 76 protein-coding genes, 38 tRNA 8 rRNA genes. The phylogenetic tree further revealed that P. wilsonii showed sister relationship with...
Abstract Phalaenopsis orchids are popular ornamental plants worldwide. The application of the efficient multiplex genome editing tools in , will greatly accelerate development orchid gene function and breeding research. In this study, we establish a fast convenient protoplast platform for identification functional tools. Two tools, PTG-Cas9 (PTG, polycistronic tRNA gRNA) system PTGm-Cas9 (PTG-Cas9 with modified sgRNA structure) designed to edit PDS commercial ST166 at four target sites. We...
Summary Clean and sustainable H 2 production is essential toward a carbon-neutral world. generation by Chlamydomonas reinhardtii an attractive approach for solar-H from O. However, it currently not scalable because of lacking ideal strains. Here, we explore hpm91 , previously reported PGR5-deletion mutant with remarkable production, that possesses numerous valuable attributes towards large-scale application in-depth study issues. We show at least 100-fold (upto 10 liter) collection sustained...
Abstract Phalaenopsis wilsonii is a typical deciduous species in the horticulturally well-known genus, . Tshi belonging to section Aphyllae moth orchid, and endemic South China. Although display feature that unique this their genetic information still insufficient limited them as breeding parent orchid. Here, we reported characterized complete chloroplast genome for We found total size of was 145,373 bp, constituting large single copy (LSC) region (84,996 bp), small single-copy (10,668 bp)...