A5028885492- Hepatitis Viruses Studies and Epidemiology
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- interferon and immune responses
- Viral Infections and Immunology Research
- Hepatitis B Virus Studies
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Animal Virus Infections Studies
- Macrophage Migration Inhibitory Factor
- Plant pathogens and resistance mechanisms
- Research on Leishmaniasis Studies
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- Computational Drug Discovery Methods
- Cytokine Signaling Pathways and Interactions
- Plant Parasitism and Resistance
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Insect Pest Control Strategies
- Pneumocystis jirovecii pneumonia detection and treatment
- SARS-CoV-2 and COVID-19 Research
- CAR-T cell therapy research
Erasmus MC
2019-2021
Lanzhou Veterinary Research Institute
2015-2017
Chinese Academy of Agricultural Sciences
2016-2017
Zhejiang Institute of Mechanical and Electrical Engineering
1981
Noroviruses are the main causative agents of acute viral gastroenteritis, but host factors that restrict their replication remain poorly identified. Guanylate-binding proteins (GBPs) interferon (IFN)-inducible GTPases exert broad antiviral activity and important mediators defenses against infections. Here, we show both IFN-γ stimulation murine norovirus (MNV) infection induce GBP2 expression in macrophages. Results from loss- gain-of-function assays indicated is for IFN-γ-dependent anti-MNV...
Abstract Background and Aims HEV infection is the most common cause of liver inflammation, but pathogenic mechanisms remain largely unclear. We aim to explore whether activates inflammasomes, crosstalk with antiviral interferon response, potential therapeutic targeting. Approach Results measured IL‐1β secretion, hallmark inflammasome activation, in serum HEV‐infected patients rabbits, cultured macrophage cell lines primary monocyte‐derived macrophages. found that genotypes 3 4 rabbits...
Babesiosis is an important haemoparasitic disease, caused by the infection and subsequent intra-erythrocytic multiplication of protozoa genus Babesia that impacts livestock industry animal health. The distribution, epidemiology genetic characterization B. bigemina, bovis, ovata in cattle China as well prevalence these protozoan agents were assessed. A total 646 blood specimens from cattle, dairy yaks 14 provinces collected tested for presence three species via a specific nested PCR assay...
Exposure to hepatitis E virus (HEV) bears a high risk of developing chronic infection in immunocompromised patients, including organ transplant recipients and cancer patients. We aim identify effective anti-HEV therapies through screening repurposing safe-in-human broad-spectrum antiviral agents. In this study, drug library comprising 94 agents was used. Upon screening, we identified gemcitabine, widely used anti-cancer drug, as potent inhibitor HEV replication. The effect confirmed range...
Although cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) signaling has been well recognized in defending DNA viruses, the role cGAS-STING regulating infection RNA viruses remains largely elusive. Noroviruses, as single-stranded are main causative agents acute viral gastroenteritis worldwide. This study comprehensively investigated response to murine norovirus (MNV) infection. We found that STING agonists potently inhibited MNV replication mouse macrophages partially...
Human coronavirus NL63 (HCoV-NL63) mainly affects young children and immunocompromised patients, causing morbidity mortality in a subset of patients. Since no specific treatment is available, this study aims to explore the anti-SARS-CoV-2 agents including favipiravir remdesivir for treating HCoV-NL63 infection. We first successfully modelled 3D structure RNA-dependent RNA polymerase (RdRp) based on experimentally solved SARS-CoV-2 RdRp structure. Molecular docking indicated that has similar...
Enteric viruses including hepatitis E virus (HEV), human norovirus (HuNV), and rotavirus are causing global health issues. The host interferon (IFN) response constitutes the first-line defense against viral infections. Melanoma Differentiation-Associated protein 5 (MDA5) is an important cytoplasmic receptor sensing infection to trigger IFN production, on other hand it also IFN-stimulated gene (ISG). In this study, we investigated effects mode-of-action of MDA5 enteric viruses. We found that...
In China, ovine babesiosis is one of the most important tick-borne haemoparasitic diseases small ruminants. It has a significant economic impact, and several Babesia motasi-like isolates have been recently shown to be responsible for in this country. Full-length C-terminal-truncated forms rap-1a61-1 gene sp. BQ1 (Lintan) were cloned into pET-30a plasmid subsequently expressed as His-fusion proteins. The resulting recombinant RAP-1a proteins (rRAP-1a61-1 rRAP-1a61-1/CT) purified evaluated...
The inflammasome machinery has recently been recognized as an emerging pillar of innate immunity. However, little is known regarding the interaction between classical interferon (IFN) response and activation in to norovirus infection. We found that murine (MNV-1) infection induces transcription IL-1β, a hallmark activation, which further increased by inhibition IFN response, but fails trigger release mature IL-1β. Interestingly, pharmacological inhibitors do not affect viral replication,...
Piroplasmosis, a disease of domestic and wild animals, is caused by tick-borne protozoa in the genera Theileria Babesia. There limited information available about prevalence piroplasmosis ticks China, to assess potential threat we investigated infections ovine bovine Babesia species collected from cattle, yaks, sheep, horses, camels several regions China where diseases have been reported. In total, 652 were animals 6 provinces China. spp. detected with PCR-RLB method identified sequencing....
Hepatitis E virus (HEV) infection is the leading cause of acute hepatitis worldwide and can develop into chronic in immunocompromised patients, promoting development effective antiviral therapies. In this study, we performed a screening library containing over 1000 FDA-approved drugs. We have identified deptropine, classical histamine H1 receptor antagonist used to treat asthmatic symptoms, as potent inhibitor HEV replication. The anti-HEV activity deptropine appears dispensable pathway, but...
A stump-tailed monkey, newly caught and without antibody to hepatitis B virus (HAV), was successfully infected with human HAV. The following alterations were observed in the monkey's functions: (1) elevation activities of serum glutamic pyruvic transaminase, lactate dehydrogenase, its type 5 isoenzyme (electrophoretically fastest moving); (2) development HAV; (3) shedding HAV antigen feces. isolated from designated Hangzhou A-1A strain HAV, serially transmitted two other monkeys. Thus,...
Serial stools and sera from 13 patients with hepatitis A were collected during an epidemic in 1978 a rural village near Hangzhou, China, studied to determine the patterns of shedding antigen (HAAg) antibody response some cases. Of 44 stool specimens, 31 HAAg-positive by radioimmunoprecipitation-polyethylene glycol assay. The highest percentage HAAg positivity was one week before after peak elevation serum glutamic pyruvic transaminase (SGPT) levels, each patient usually occurred early...
SUMMARY Ovine babesiosis is one of the most important tick-borne haemoparasitic diseases small ruminants. The ovine parasite Babesia sp. Xinjiang widespread in China. In this study, recombinant full-length XJrRAP-1aα2 (rhoptry-associated protein 1aα2) and C-terminal CT were expressed used to evaluate their diagnostic potential for infections by indirect enzyme-linked immunosorbent assay (ELISA). Purified was tested reactivity with sera from animals experimentally infected other...
Noroviruses are the main causative agents for acute viral gastroenteritis worldwide. RIG-I-like receptors (RLRs) triggered interferon (IFN) activation is essential host defense against infections. In turn, viruses have developed sophisticated strategies to counteract antiviral response. This study aims investigate how murine norovirus (MNV) replicase interacts with RLRs-mediated IFN Counterintuitively, we found that MNV NS7 enhances of poly (I:C)-induced response and transcription downstream...
Hepatitis E virus (HEV) infection in immunocompromised patients, pregnant women and children requires treatment; however, no approved medication is currently available. The macrolide antibiotic azithromycin has been identified as a potent HEV inhibitor. Azithromycin inhibits replication viral protein expression multiple cell culture models with genotype 1 3 strains. This largely independent of its induction an interferon-like response. Because it safe cheap, repurposing for treating...
a. Alignment of partial nucleotide sequences rap-1a gene from different Chinese isolates. FJ2: B. bovis isolate Fujian 2; GS: Guansu; FJ1: 1;GX: Guangxi; HAN: Hainan; YN1: Yunnan 1; YN2: CQ: Chongqing; HEN2: Henan HEN 1: HEN3: Henan3. Nucleotide substitutions indicated with yellow background. Among these substitutions, affected amino acid modifications red b. RAP-1a GX: Repeats in isolates are underlined. (DOC 1086Â kb)
Abstract Noroviruses are the main causative agents of acute viral gastroenteritis worldwide. However, no vaccine or specific antiviral treatment is available, imposing a heavy global health burden. The nucleoside analogue 2’-fluoro-2’-deoxycytidine (2’-FdC) has been reported to have broad activity. Here, we report that 2’-FdC significantly inhibits murine norovirus replication in macrophages. This effect was partially reversed by exogenous supplementation cytidine triphosphate. combination...