A5020015647- Kruppel-like factors research
- Pancreatic function and diabetes
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Metabolism, Diabetes, and Cancer
- Genetic Syndromes and Imprinting
- Diabetes Treatment and Management
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related gene regulation
- Alcohol Consumption and Health Effects
- Genomics, phytochemicals, and oxidative stress
- Fibroblast Growth Factor Research
- Cancer, Lipids, and Metabolism
- Glutathione Transferases and Polymorphisms
- Zebrafish Biomedical Research Applications
- Pancreatitis Pathology and Treatment
- Lipid metabolism and biosynthesis
- Genetics, Aging, and Longevity in Model Organisms
- Genetic Neurodegenerative Diseases
- Diabetes Management and Research
- Peatlands and Wetlands Ecology
University of Tsukuba
2016-2025
University of Tsukuba Hospital
2025
Murayama Medical Center
2023
Akita Prefectural University
2021
Kumamoto University
2015-2018
Keio University
2017
University of Shizuoka
2008-2010
Hepatic lipogenesis is nutritionally regulated (i.e., downregulated during fasting and upregulated the postprandial state) as an adaptation to nutritional environment. While alterations in expression level of transcription factor SREBP-1c are known be critical for lipogenesis, upstream mechanisms governing Srebf1 remain unclear. Here, we show that fasting-induced KLF15, a key regulator gluconeogenesis, forms complex with LXR/RXR, specifically on promoter. This recruits corepressor RIP140...
Maturity-onset diabetes of the young type 5 (MODY5), causally associated with loss-of-function HNF1B gene, is a rare form monogenic that has been underdiagnosed in part because microdeletions chromosome 17q12 encompassing gene cannot be detected by sequencing-based approaches, which accounts for about 50% MODY5 cases. We herein describe 37-year-old Japanese woman who manifested diabetic ketosis at onset. The coexistence features MODY5, including abnormal renal function, impaired insulin...
Abstract Low lean body mass increases fall risk. Some diabetes medications, specifically SGLT2 inhibitors and GLP-1RAs, can cause muscle loss. This study assessed their association on falls in type 2 patients. An annual survey was conducted for up to 5 years individuals with admitted our department. Fall risk factors were identified using discrete-time survival analysis. The observed 471 participants over a median period of years. had age 64 years, incidence rate 17.1 per 100 person-years....
Peroxisome proliferator-activated receptor-α (PPARα) is a therapeutic target for hyperlipidemia. K-877 new selective PPARα modulator (SPPARMα) that activates transcriptional activity. The aim of the present study was to assess effects on lipid metabolism in vitro and vivo compared with those classical agonists.To compare activity agonists Wy14643 (Wy) fenofibrate (Feno), cell-based transactivation luciferase assay carried out. WT Ppara-/- mice were fed moderate-fat (MF) diet 6 days,...
Glucocorticoids have various medical uses but are accompanied by side effects. The glucocorticoid receptor (GR) has been reported to regulate the clock genes, underlying mechanisms incompletely understood. In this study, we focused on suppressive effect of GR expression Rev-erbα (Nr1d1), an important component regulatory circuits. Here show that suppresses via formation a complex with CLOCK and BMAL1, which binds E-boxes in Nr1d1 promoter. GR-CLOCK-BMAL1 complex, does not directly bind DNA,...
Abstract Background Mild cognitive impairment (MCI) is not just a prodrome to dementia, but very important intervention point prevent dementia caused by Alzheimer's disease (AD). It has long been known that people with AD have higher frequency of falls some gait instability. Recent evidence suggests vestibular disproportionately prevalent among individuals MCI and due AD. Therefore, we hypothesized the measurement balance capability helpful identify MCI. Methods First, developed useful...
Maintenance of metabolic homeostasis is secured by metabolite-sensing systems, which can be overwhelmed constant macronutrient surplus in obesity. Not only the uptake processes but also consumption energy substrates determine cellular burden. We herein describe a novel transcriptional system this context comprised peroxisome proliferator-activated receptor alpha (PPARα), master regulator for fatty acid oxidation, and C-terminal binding protein 2 (CtBP2), corepressor. CtBP2 interacts with...
cAMP responsive element-binding protein 3 like (CREB3L3) is a membrane-bound transcription factor involved in the maintenance of lipid metabolism liver and small intestine. CREB3L3 controls hepatic triglyceride glucose by activating plasma fibroblast growth 21 (FGF21) lipoprotein lipase. In this study, we intended to clarify its effect on atherosclerosis.CREB3L3-deficifient, liver-specific knockout, intestine-specific both liver- transgenic mice were crossed with LDLR-/- mice. These fed...
During periods of fasting, the body undergoes a metabolic shift from carbohydrate utilization to use fats and ketones as an energy source, well inhibition de novo lipogenesis initiation gluconeogenesis in liver. The transcription factor sterol regulatory element‐binding protein‐1 (SREBP‐1), which plays critical role regulation lipogenesis, is suppressed during resulting suppression hepatic lipogenesis. We previously demonstrated that interaction fasting‐induced Kruppel‐like 15 (KLF15) with...
Fatty acid synthase (Fasn) is a key component of energy metabolism that dynamically induced by food intake. Although extensive studies have revealed number transcription factors involved in the fasting/refeeding transition Fasn expression hepatocytes, much less evidence available for adipocytes. Using vivo Ad-luc analytical system, we identified inverted CCAAT element (ICE) around -100 nucleotides promoter as critical cis-element refeeding response Electrophoretic mobility shift assays and...
Mice overexpressing the nuclear form of CREBH mainly in liver (CREBH-Tg) showed suppression high-fat high-sucrose (HFHS) diet-induced obesity accompanied by an increase plasma fibroblast growth factor 21 (FGF21) levels. overexpression induced browning inguinal white adipose tissue (WAT) and whole-body energy expenditure, which was canceled Fgf21−/− mice. Deficiency FGF21 CREBH-Tg mice mostly improvement obesity, but inflammation epidermal WAT, amelioration insulin resistance, glucose...
A high-fat diet is thought to enhance inflammation in various tissues by increasing insulin resistance. In this study, we determined the mRNA levels of inflammatory cytokines leukocyte-derived cells blood rats with high-fat-diet-induced Feeding a for 77 d induced moderate resistance, which was increased plasma glucose and concentrations, following an oral tolerance test. The interleukin (IL)-1β level higher insulin-resistant than control at fasting stage, whereas tumor necrosis factor...
Abstract The endoplasmic reticulum (ER)–embedded transcription factors, sterol regulatory element-binding proteins (SREBPs), master regulators of lipid biosynthesis, are transported to the Golgi for proteolytic activation tune cellular cholesterol levels and regulate lipogenesis. However, mechanisms by which cell responds saturated or unsaturated fatty acids remain underexplored. Here, we show that RHBDL4/RHBDD1, a rhomboid family protease, directly cleaves SREBP-1c at ER. p97/VCP,...
KLF15 is a transcription factor that plays an important role in the activation of gluconeogenesis from amino acids as well suppression lipogenesis glucose. Here we identified start site liver-specific transcript and showed FoxO1/3 transcriptionally regulates Klf15 gene expression by directly binding to promoter. To achieve this, performed precise vivo promoter analysis combined with genome-wide transcription-factor-screening method "TFEL scan", using our original Transcription Factor...
The adaptive increase in insulin secretion early stages of obesity serves as a safeguard mechanism to maintain glucose homeostasis that cannot be sustained, and the eventual decompensation β cells is key event pathogenesis diabetes. Here we describe crucial system orchestrated by transcriptional cofactor CtBP2. In cultured cells, gene expression coactivated Global genomic mapping CtBP2 binding sites identifies interaction between NEUROD1 through which decompacts chromatin promoter....
It is well-known that insulin resistance induces lipid abnormalities by decreasing actions in adipose tissue. This study examined the effects of inhibiting postprandial hyperglycemia/hyperinsulinemia, using alpha-amylase inhibitor wheat albumin (WA), on expression genes related to fatty acid metabolism tissue high-fat diet-induced insulin-resistant rats. Postprandial glucose and levels were significantly lower after oral starch loading with WA than inactivated In addition, increases plasma...
Abstract Background Renal hypouricemia (RHUC) is a hereditary disorder where mutations in SLC22A12 gene and SLC2A9 cause RHUC type 1 (RHUC1) 2 (RHUC2), respectively. These genes regulate renal tubular reabsorption of urates while there exist other counterbalancing the net excretion including ABCG2 SLC17A1 . Urate metabolism tightly interconnected with glucose metabolism, may be involved insulin secretion from pancreatic β-cells. On hand, myriad are responsible for impaired independently...