A5082114367- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- RNA modifications and cancer
- Plant Molecular Biology Research
- RNA Interference and Gene Delivery
- Lanthanide and Transition Metal Complexes
- Invertebrate Immune Response Mechanisms
- Photosynthetic Processes and Mechanisms
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Plant Reproductive Biology
- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Hydrology and Watershed Management Studies
- Advanced biosensing and bioanalysis techniques
- Flood Risk Assessment and Management
- Immune cells in cancer
- High-Voltage Power Transmission Systems
- Virus-based gene therapy research
- Receptor Mechanisms and Signaling
- Antimicrobial Peptides and Activities
- Axon Guidance and Neuronal Signaling
- Galectins and Cancer Biology
- Phagocytosis and Immune Regulation
Zhejiang University
2018-2025
Harbin Institute of Technology
2021-2023
Heilongjiang Institute of Technology
2021-2023
Personalized neoantigen cancer mRNA vaccines are promising candidates for precision medicine. However, the difficulty of identifying neoantigens heavily hinders their broad applicability. This study developed a universal strategy anti-tumor vaccine by harnessing "off-the-shelf" immunity to known antigens. First, model antigen ovalbumin (OVA) is used design. In vitro test indicated that this reprogrammed tumor cells can be recognized and killed OVA-specific cytotoxic T lymphocytes (CTLs)....
The vibration issue of pumped storage power station unit will directly affect the safe and stable operation unit, pressure pulsation in vaneless region is an important index to evaluate stability unit. In order study fluctuation characteristics pump-turbine turbine mode, unsteady numerical simulation maximum head condition rated carried out this research. amplitude-frequency under different influence internal flow on are analyzed. results show that non-uniform field formed due rotor-stator...
Drosophila melanogaster Down syndrome cell adhesion molecule (Dscam1) can generate 38,016 different isoforms through largely stochastic, yet highly biased, alternative splicing. These are required for nervous functions. However, the functional significance of splicing bias remains unknown. Here, we provide evidence that Dscam1 is mushroom body (MB) axonal wiring. We mutant flies with normal overall protein levels and an identical number but global changes in exon 4 9 isoform (DscamΔ4D-/-...
The Down syndrome cell adhesion molecule 1 ( Dscam1 ) gene can generate tens of thousands isoforms via alternative splicing, which is essential for nervous and immune functions. Chelicerates approximately 50 to 100 shortened Dscam (sDscam) by promoters, similar mammalian protocadherins. Here, we reveal that trans-splicing markedly increases the repository sDscamβ in Tetranychus urticae . Unexpectedly, every variable exon cassette engages with constant exons from another cluster. Moreover,...
Drosophila melanogaster Dscam1 encodes 38,016 isoforms via mutually exclusive splicing; however, the regulatory mechanism behind this is not fully understood. Here, we found a set of hidden RNA secondary structures that balance stochastic choice splice variants (designated balancer structures). In vivo mutational analyses revealed dual function these interactions in driving variants, through enhancement inclusion distal exon 6s by cooperating with docking site-selector pairing to form...
The immunoglobulin (Ig) superfamily receptor Down syndrome cell adhesion molecule (Dscam) gene can generate tens of thousands isoforms via alternative splicing, which is essential for both nervous and immune systems in insects. However, further information required to develop a comprehensive view Dscam diversification across the broad spectrum Chelicerata clades, basal branch arthropods second largest group terrestrial animals. In this study, genome-wide analysis genes species revealed burst...
Alternative splicing of Drosophila Dscam1 into 38,016 isoforms provides neurons with a unique molecular code for self-recognition and self-avoidance. A canonical model suggests that the homophilic binding identical on sister branches mushroom body (MB) axons supports segregation high fidelity, even when only single isoform is expressed. Here, we generated series mutant flies exon 4, 6, or 9 variant, encoding 1,584, 396, 576 potential isoforms, respectively. Surprisingly, most mutants in...
Abstract Drosophila Down syndrome cell adhesion molecule 1 (Dscam1) encodes tens of thousands recognition molecules via alternative splicing, which are required for neural function. A canonical self-avoidance model seems to provide a central mechanistic basis Dscam1 functions in neuronal wiring. Here, we reveal extensive noncanonical isoforms We generated series allelic cis mutations Dscam1, encoding normal number isoforms, but with an altered isoform composition. Despite dendritic and...
Alternative pre-mRNA splicing remarkably expands protein diversity in eukaryotes. Drosophila PGRP-LC can generate three major 3' splice isoforms that exhibit distinct innate immune recognition and defenses against various microbial infections. However, the regulatory mechanisms underlying uniquely biased pattern at variable region remain unclear. Here we show competing RNA pairings control unique of pre-mRNA. We reveal roles by which these jointly regulate variant selection through...
Abstract The research on water and sediment variations is of great importance for regional resource watershed management. Studying the relationship between precipitation, runoff load, traditional methods are based observational data fitted or artificial intelligence predictions, whose accuracy directly dependent quality quantity data. This paper developed a new approach researching load. Based sliding accumulative anomaly method, M-K mutation analysis wavelet functions, variation...
Abstract Unstable operation during the no-load startup of pumped storage hydroelectric power plants is a significant factor affecting successful grid connection units. To investigate internal flow and pressure pulsation characteristics pump-turbine under varying heads, vertical single-stage mixed-flow reversible studied. Numerical simulations are performed using SST k-ω turbulence model, Fast Fourier Transform (FFT) method employed to analyze signals. A comparative study conducted on...
Drosophila melanogaster Down syndrome cell adhesion molecule 1 (Dscam1) encodes 19,008 diverse ectodomain isoforms via the alternative splicing of exon 4, 6, and 9 clusters. However, whether individual or clusters have specific significance is unclear. Here, using phenotype-diversity correlation analysis, we reveal redundant roles Dscam1 diversity in neuronal wiring. A series deletion mutations were performed from endogenous locus harboring clusters, reducing to 396 18,612 potential...
Mutually exclusive splicing is an important mechanism for expanding protein diversity. An extreme example the Down syndrome cell adhesion molecular ( Dscam1 ) gene of insects, containing four clusters variable exons (exons 4, 6, 9, and 17), which potentially generates tens thousands isoforms through mutually splicing, regulatory mechanisms are still elusive. Here, we systematically analyzed exon 9 in Coleoptera species. Through comparative genomics RNA secondary structure prediction, found...
SUMMARY Alternative splicing of Drosophila Dscam1 into 38,016 isoforms provides neurons with a unique molecular code for self-recognition and self-avoidance. A canonical model suggests that homophilic binding identical on sister branches mushroom body (MB) axons supports segregation high fidelity, even when only single isoform is expressed. Here we generated series mutant flies exon 4, 6, or 9 variant, encoding 1,584, 396, 576 potential isoforms, respectively. Surprisingly, most the mutants...
Abstract Drosophila melanogaster Down syndrome cell adhesion molecule ( Dscam1 ) can potentially generate 38,016 different isoforms through stochastic, yet highly biased, alternative splicing. Genetic studies demonstrated that stochastic expression of multiple provides each neuron with a unique identity for self/non-self-discrimination. However, due to technical obstacles, the functional significance specific bias in isoform remains entirely unknown. Here, we provide conclusive evidence...
Abstract Drosophila Dscam1 encodes ten thousand of cell recognition molecules via alternative splicing, which is required for nervous function. However, the underlying mechanism has not fully understood. Here we revealed extensive noncanonical functions isoform diversity in neuronal wiring. We generated a series allelic cis mutations Dscam1, encoding normal number isoforms albeit with altered composition. Despite dendritic self-avoidance and self/non-self discrimination da neurons,...
<p indent="0mm">mRNA-based therapeutics are widely used for treating various diseases and represent an emerging preventive therapeutic approach that can be effective alternative to DNA- recombinant protein-based therapeutics. Accompanied by the continuous development of <italic>in vitro</italic> synthesis, purification, modification, delivery system optimization, mRNA-based have been substantially improved in terms stability, translation efficiency, controllable immunogenicity. At present,...