Berberine, a natural isoquinoline alkaloid isolated from the berberis species, has wide array of biological properties such as anti-inflammatory, antibacterial, antifungal, and antihelminthic effects. We evaluated antiviral effect berberine against influenza A/FM1/1/47 (H1N1) in vivo vitro. The results showed that strongly suppressed viral replication A549 cells mouse lungs. Meanwhile, relieved pulmonary inflammation reduced necrosis, inflammatory cell infiltration, edema induced by...

Rheumatoid arthritis is a common autoimmune disease linked to chronic inflammation. Dysbiosis of the gut microbiota has been proposed contribute risk RA, and large number researchers have investigated gut–joint axis hypothesis using collagen-induced rats. However, previous studies mainly involved short-term experiments; very few used CIA model investigate changes in over time. Moreover, research failed use carry out detailed investigations effects drug treatments upon inflammation joints,...

The mucosa of vertebrates is a particularly complex but dynamic environment in which the host constantly interacts with trillions commensal microorganisms and pathogens. Although internal external mucosal microbiomes immune defense mammals have been well investigated, relationship between microbes their host’s responses has not systematically understood early vertebrates. In this study, we compared composition distribution microbiota common carp ( Cyprinus carpio ), found that there were...

Hazardous impacts caused by harmful algal blooms (HABs) have facilitated the development of novel effective approaches for controlling frequent HABs. Recently, bacteria with algicidal effects, as a promising alternative tool eliminating HABs-forming species received wide concerns. In present study, marine bacterial strain 6A1 potent activity was successfully isolated from bloom Akashiwo sanguinea , and characteristics related to its action were systematically investigated. Sequencing results...

Personalized neoantigen cancer mRNA vaccines are promising candidates for precision medicine. However, the difficulty of identifying neoantigens heavily hinders their broad applicability. This study developed a universal strategy anti-tumor vaccine by harnessing "off-the-shelf" immunity to known antigens. First, model antigen ovalbumin (OVA) is used design. In vitro test indicated that this reprogrammed tumor cells can be recognized and killed OVA-specific cytotoxic T lymphocytes (CTLs)....

Antibiotics, as the most commonly prescribed class of drugs in neonatal intensive care units, have an important impact on developing gut microbiota. Therefore, comprehending effects used antibiotic therapy microbiota and butyrate-producers early infants could provide information for therapeutic decision-making NICU. To explore development infants. A total 72 were included study. We performed 16S rRNA sequencing stool swab samples collected from unit patients who received...

Background: Chronic migraine (CM) is a common complex nervous system disease, often accompanied by symptoms of the digestive tract that interact with each other, leading to prolonged and difficult-to-cure migraines. These are associated abnormalities in 5-HT its receptors. Wuzhuyu decoction (WZYD) traditional Chinese medicine prescription commonly used clinics treat CM; it relieves gastrointestinal symptoms, such as nausea vomiting; however, mechanism still unclear. Investigating differences...

Forsythiaside A, a phenylethanoid glycoside monomer extracted from Forsythia suspensa, shows anti-inflammatory, anti-infective, anti-oxidative, and antiviral pharmacological effects. The precise mechanism underlying the action of forsythiaside A is not completely clear. Therefore, in this study, we aimed to determine whether anti-influenza occurs via retinoic acid-inducible gene-I-like receptors (RLRs) signaling pathway lung immune cells. was used treat C57BL/6J mice MAVS-/- infected with...

Influenza virus is a single-stranded RNA that causes influenza in humans and animals. About 600 million people around the world suffer from every year. Upon recognizing viral molecules, TLR7 (Toll-like receptor) initiates corresponding immune responses. Traditional Chinese Medicines (TCMs), including Yinqiao powder, Xinjiaxiangruyin Guizhi-and-Mahuang decoction, have been extensively applied clinical treatment of influenza. Although therapeutic efficacy TCMs against vivo was reported...

Chuanxiong Rhizoma and Cyperi (CRCR), an ancient classic formula comprised of in a weight ratio 1:2, has long been used for curing migraine. This study aimed to explore their anti-migraine effect active constituents. A nitroglycerin (NTG)-induced migraine model rats was established evaluate pharmacological effects. Cerebral blood flow detected by laser Doppler perfusion monitor. The levels endothelin-1 (ET-1), γ-aminobutyric acid (GABA), nitric oxide synthase (NOS), (NO), 5-hydroxytryptamine...

Acquired treatment resistance is an important cause of death in prostate cancer, and this study aimed to explore the mechanisms chemotherapy cancer. We employed castration-resistant cancer (CRPC), neuroendocrine (NEPC), chemotherapy-resistant datasets screen for potential target genes. The Cancer Genome Atlas (TCGA) was used detect correlation between genes prognosis clinical characteristics. Nei endonuclease VIII-like 3 (NEIL3) knockdown cell lines were constructed with RNA interference....

Polyplexes are required to be equipped with multiple functionalities accomplish adequate structure stability and gene transfection efficacy for therapy. Herein, a 4-carboxy-3-fluorophenylboronic acid (FPBA)-functionalized block copolymer of PEG-

Wuzhuyu decoction (WZYD) has been clinically used to treat migraine effectively since Eastern Han Dynasty of ancient China. However, its antimigrainic ingredients remain unclear. In present study, the WZYD were explored and optimized in nitroglycerin-induced rats through correlation analysis spectra-pharmacological effects absorption using entropy-weighted partial least squares regression method. The spectra obtained determination nine main ten kinds WZYDs WZYDs' single-pass intestinal...

It is widely understood that commensal microbiota contributes to the maintenance of intestinal homeostasis through dynamic interactions with a body's immunity. And immune regulation important for influenza vaccine's effectiveness after body injection, however, mechanism between and remains unknown. The impact individual bacteria species have on balance systemic system beyond local mucosal tissues also less clear, related still In this study, administration various antibiotics, we examined...

Migraine is a common neurological disorder that manifests as recurrent attacks of unilateral and throbbing headache. Conioselinum anthriscoides "Chuanxiong" (Apiaceae; Chuanxiong rhizoma) Cyperus rotundus L. (Cyperaceae; Cyperi (CRCR), classic prescription for treating migraine. This study aimed to reveal the potential mechanisms CRCR extract against migraine using integrated analysis metabolomics network pharmacology. Behavioral changes in nitroglycerin rat model were determined from von...

In previous study, we reported that 14-kDa phosphohistidine phosphatase (PHP14) was associated with lung cancer cell migration and invasion. We also found the expression of PHP14 markedly increased in a part human tissues. this investigated impact knockdown on tumorigenesis vitro vivo, as well regulatory pathway. Depletion endogenous cells reduced colony formation activity inhibited xenograft tumor growth vivo. Further experiments revealed NF-κB signal pathway inhibitor PDTC upregulated MMP9...

The crosstalk between the immune system and microbiota drives an amazingly complex mutualistic symbiosis. In mammals, upper respiratory tract acts as a gateway for pathogen invasion, dynamic interaction mucosal immunity on its surface can effectively prevent disease development. However, relationship virus-mediated responses microbes in lower vertebrates remains uncharacterized. this study, we successfully constructed infection model by intraperitoneally injecting common carp ( Cyprinus...

Objective . We wished to investigate the effects of traditional Chinese medicine Gui Zhi Ma Huang Ge Ban Tang on controlling influenza A virus (IAV) infection and improving inflammation in mouse lungs. Method Mice were maintained normal cold environments infected with IAV by intranasal application, respectively. Real-time quantitative polymerase chain reaction was used measure mRNA expression TLR7, myeloid differentiation primary response 88 (MyD88), nuclear factor-kappa B (NF- κ B)p65 TLR7...