Lipid nanoparticle (LNP) is commonly used to deliver mRNA vaccines. Currently, LNP optimization primarily relies on screening ionizable lipids by traditional experiments which consumes intensive cost and time. Current study attempts apply computational methods accelerate the development for Firstly, 325 data samples of vaccine formulations with IgG titer were collected. The machine learning algorithm, lightGBM, was build a prediction model good performance (R2 > 0.87). More importantly,...

Lipid nanoparticle (LNP)-based mRNA therapeutics, highlighted by the success of SARS-CoV-2 vaccines, face challenges due to inflammation caused ionizable lipids. These lipids can activate immune system, particularly when co-delivered with nucleic acids, leading undesirable inflammatory responses. We introduce a novel class anti-inflammatory functionalized hydroxychloroquine (HCQ), which suppresses both lipid-induced and acid-induced activation. HCQ-functionalized LNPs (HL LNPs) exhibit...

Nonbifurcating divergence caused by introgressive hybridization is continuously reported for groups of closely related species. In this study, we aimed to reconstruct the genome-scale classification deep lineages conifer genus Picea, establish their phylogenetic relationships and test bifurcating hypothesis between deeply branching based on genomic data. We sequenced transcriptomes 35 individuals 27 taxa covering all main genus. Four major lineages, comprising three 12 each, largely...

2-hydroxybutyric acid (2HB) serves as an important regulatory factor in a variety of diseases. The circulating level 2HB serum is significantly higher multiple diseases, such cancer and type 2 diabetes (T2D). However, there currently no systematic study on 2HB-producing bacteria that demonstrates whether gut contribute to the pool. To address this question, we used BLASTP reveal taxonomic profiling human microbiome, which are mainly distributed phylum Proteobacteria Firmicutes. In vitro...

Abstract Bile salt hydrolases (BSHs) are enzymes that essential for the enterohepatic metabolism of bile acids (BAs). BSHs catalyze production unconjugated BAs and regulate homeostasis BA pool. This study identified Lactobacillus as a crucial BSH‐encoding genus, 16 main species were obtained using metagenomic data from publicly available human gut microbiome databases. Then, lactobacilli classified into four typical categories by BSH phylotypes, including five encoding BSH‐T0, six BSH‐T2,...

Speciation among populations connected by gene flow is driven adaptation to different environments, but underlying gene-environment associations remain largely unknown. Here, 162 individuals from 32 were sampled obtain 191,648 independent single nucleotide polymorphisms (SNPs) across the genomes of two closely related spruce species, Picea asperata and crassifolia, which occur on Qinghai-Tibet Plateau in surrounding regions. Using SNP data set, genotype-environment demographic modelling used...

Abstract Lepus oiostolus ( L . ) is a species endemic to the Qinghai-Tibet Plateau. However, absence of reference genome limits genetic studies. Here, we reported high-quality assembly, with scaffolds anchored 24 chromosomes and total assembled length 2.80 Gb (contig N50 = 64.25 Mb). Genomic annotation uncovered 22,295 protein-coding genes identified 49.84% sequences as transposable elements. Long interspersed nuclear elements (LINEs) constitute high proportion genome. Our study at first...

The bar-headed goose (Anser indicus) mainly inhabits the plateau wetlands of Asia. As a specialized high-altitude species, geese can migrate between South and Central Asia annually fly twice over Himalayan mountains along central Asian flyway. physiological, biochemical behavioral adaptations to living flying have raised much interest. However, date, there is still no genome assembly information publicly available for geese. In this study, we present first de novo whole sequencing goose,...

Objective: We aimed to characterize the epidemiological and clinical characteristics of sporadic Creutzfeldt-Jakob disease (sCJD) in eastern China this retrospective study. Methods: This study enrolled 67 patients with sCJD hospitalized a grade-A tertiary hospital from January 2010 2020. Demographic data, symptoms, brain magnetic resonance imaging (MRI), electroencephalogram (EEG), cerebrospinal fluid (CSF) 14-3-3 protein test, polymerase chain reaction (PCR), DNA sequence determination...

Global climate change poses a grave threat to biodiversity and underscores the importance of identifying genes corresponding environmental factors involved in adaptation tree species for purposes conservation forestry. This holds particularly true spruce species, given their pivotal role as key constituents montane, boreal, sub-alpine forests Northern Hemisphere.Here, we used transcriptomes, occurrence records, data investigate spatial genetic distribution climate-associated variation Picea...

The rice stem borer, Chilo suppressalis Walker, is one of the most important global agricultural pests. C. has distinct and water-oat host populations. Asynchrony in sexual activity thought to be main factor maintaining reproductive segregation between these populations, particularly obvious difference circadian rhythm female calling However, mechanism responsible for this timing poorly understood. clock an essential regulator daily behavioral rhythms insects, including calling. We...

3例患儿临床以发育迟缓、癫痫发作伴不同程度运动障碍为主要表现，基因全外显子测序均为DHDDS基因变异，均诊断为DHDDS基因相关发育迟缓和癫痫伴或不伴运动障碍。2例患儿呈神经退行性病程，癫痫起病年龄分别为1岁2月龄及3岁，发作形式包括肌阵挛、强直阵挛及动作减少性发作，1例伴有热敏感表现。.

The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A•GTP•tRNA complex to deliver aminoacyl-tRNA site of ribosomes.This study described cDNA sequences and partial genomic structure porcine EEF1A1.The EEF1A1 gene encoded a with 462 amino acids, which shared complete homology human, chimpanzee dog.The temporal expression pattern showed diversity level mRNA was relatively minor prenatal embryo skeletal muscle, however, decreased during aging after...

This study provides a mitochondrial complete genome of Gymnocypris waddellii. The is 16,674 bp in length with an A + T content 55.4%, which contains 13 protein-coding genes, 22 tRNA and 2 rRNA genes. phylogenetic analysis indicated that G. waddellii closely related to Oxygymnocypris stewartii. These results contribute explore the molecular biology characteristic on conditions Qinghai-Tibet Plateau.

<p indent="0mm">mRNA-based therapeutics are widely used for treating various diseases and represent an emerging preventive therapeutic approach that can be effective alternative to DNA- recombinant protein-based therapeutics. Accompanied by the continuous development of <italic>in vitro</italic> synthesis, purification, modification, delivery system optimization, mRNA-based have been substantially improved in terms stability, translation efficiency, controllable immunogenicity. At present,...

Incarvillea younghusbandii Sprague is a traditional tonic herb. The roots are used as herbal medicine for nourishing and strengthening, well treating postpartum milk deficiency weakness. In this study, the chloroplast genome of I. was sequenced assembled by high-throughput sequencing technology. sequence characteristics, repeats, codon usage bias, phylogenetic relationships estimated divergence time were analyzed. 159 323 bp contained large single copy (80 197 bp), small (9 030 bp) two...

The DEPDC5 gene (OMIM 614191) has been proven to be a frequent cause of familial and sporadic focal epilepsy. A human induced pluripotent stem cell (iPSC) line was generated from child diagnosed with epilepsy, which caused by DEDPC5 mutation. iPSC expresses high pluripotency markers, carries the mutation, can differentiate into three germ layers in vitro. lines offer promising technique for studying pathogenesis conducting drug screening DEDPC5-related

Lepus oiostolus (L. oiostolus) is a species endemic to the Qinghai-Tibet Plateau. However, absence of reference genome limits genetic studies. Here, we report high-quality L. assembly, with scaffolds anchored 24 chromosomes and total assembled length 2.80 Gb (contig N50 = 64.24 Mb). We found that transposable elements account for 49.84% genome, 22,295 predicted protein-coding genes. Long interspersed nuclear (LINEs) constitute high proportion their expansion key contributor this species’s...

Objective To evaluate the application value of whole exome sequencing (WES) in diagnosis NDDs (neuro-developmental disorders) children. Metheod WES was used for 35 unexplained NDD children, which admitted to outpatient and ward Children′s hospital affiliated Capital institute pediatric from November 2015 2016. These children′s clinical data collected detailedly. Using bioinformatics software tools combining with patient′s phenotype, candidate genetic/genomic variants these patients...