A5016351638- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- RNA modifications and cancer
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Animal Genetics and Reproduction
- Nematode management and characterization studies
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Protist diversity and phylogeny
- Prenatal Screening and Diagnostics
- Bacteriophages and microbial interactions
- Plant pathogens and resistance mechanisms
- RNA and protein synthesis mechanisms
- Genetics, Bioinformatics, and Biomedical Research
AgroParisTech
2012-2022
Institut Jean-Pierre Bourgin
2011-2022
Université Paris-Saclay
2016-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2008-2022
Centre National de la Recherche Scientifique
2016-2020
Institut National de la Recherche Agronomique du Niger
2014
Institut National de la Recherche Agronomique
1997-2009
Laboratoire de Génétique Cellulaire
1985-2007
Université de Pau et des Pays de l'Adour
2004
University of Wisconsin System
2000
*Transposable elements (TE) induce structural and epigenetic alterations in their host genome, with major evolutionary implications. These are examined here the context of allopolyploid speciation, on recently formed invasive species Spartina anglica, which represents an excellent model to contrast plant genome dynamics following hybridization doubling natural conditions. *Methyl-sensitive transposon display was used investigate TE insertion sites for several elements, it comparable...
• Analyses of selected bacterial artificial chromosomes (BACs) clones suggest that the retrotransposon component angiosperm genomes can be amplified or deleted, leading to genome turnover. Here, Nicotiana allopolyploids were used characterize nature sequence turnover across whole in known different ages. Using molecular-clock analyses, likely age was estimated. Genomic situ hybridization (GISH) and tandem repeat characterization determine how parental genomic compartments these have diverged...
We used next generation sequencing to characterize and compare the genomes of recently derived allotetraploid, Nicotiana tabacum (<200,000 years old), with its diploid progenitors, sylvestris (maternal, S-genome donor), tomentosiformis (paternal, T-genome donor). Analysis 14,634 repetitive DNA sequences in progenitor species N. reveal all major types retroelements found angiosperms (genome proportions range between 17–22.5% 2.3–3.5% for Ty3-gypsy elements Ty1-copia elements, respectively)....
The genomes of most Nicotiana species contain three different subfamilies the Tnt1 retrotransposon, which differ completely in their U3 sequence, whereas rest sequence is relatively constant. results presented here show that all are expressed tobacco (Nicotiana tabacum) and variability correlates with differences pattern expression elements. Each induced by stress, but promoters have a response to stress-associated signaling molecules. Tnt1A subfamily particularly strongly elicitors methyl...
Transposable elements (TEs) are mobile DNA sequences present in the genomes of most organisms. They have been extensively studied animals, fungi, and plants, shown to important functions genome dynamics species evolution. Recent genomic data can now enlarge identification study TEs other branches eukaryotic tree life. Diatoms, which belong heterokont group, unicellular algae responsible for around 40% marine primary productivity. The a centric diatom, Thalassiosira pseudonana, pennate...
Recent advances have highlighted the ubiquity of whole-genome duplication (polyploidy) in angiosperms, although subsequent genome size change and diploidization (returning to a diploid-like condition) are poorly understood. An excellent system assess these processes is provided by Nicotiana section Repandae, which arose via allopolyploidy (approximately 5 million years ago) involving relatives sylvestris obtusifolia. Subsequent speciation Repandae has resulted allotetraploids with divergent...
The Tntl retrotransposon was isolated from tobacco after transposition.Tntl expression is very low at the plant level and under most stress condlUons but strongly induced during protoplast Isolation.It shown here that tranecription of ratrotreneposon can be activated by several microbial factors having common ability to elicit a hypersensitive response in tobacco.These elicitore Include Onozuka crude extracts fungus Trlchoderma v/ride, ellcitins purified Phytophthora fungsl species culture...
Tandemly arranged nuclear ribosomal DNA (rDNA), encoding 18S, 5.8S and 26S RNA (rRNA), exhibit concerted evolution, a pattern thought to result from the homogenisation of rDNA arrays. However homogeneity at single nucleotide polymorphism (SNP) level has not been detailed in organisms with more than few hundred copies unit. Here we study complexity species arrays consisting thousands units.We examined genic (18S) non-coding internally transcribed spacer (ITS1) regions using Roche 454 and/or...
The tobacco Tntl retrotransposon is the only plant that has been shown to be transcriptionally active, and its transcription strongly induced when preparing leaf-derived protoplasts.We have analysed in this paper LTR sequences important for expression show upstream of TATA box are sufficient confer protoplast-dependent induction a heterologous promoter.We also region contains two short activator elements, one these sequences, BlI, interacts with protoplast-specific nuclear factors.
This paper establishes relationships between two aspects of ribosomal DNA (rDNA) biology: epigenetic silencing rDNA loci; and homogenization leading to concerted evolution. Here, we examined inheritance expression patterns in three natural Nicotiana allopolyploids (closest living descendants diploid parents are given), N. rustica (N. paniculata x undulata), tabacum sylvestris tomentosiformis) arentsii undulata wigandioides), synthetic F(1) hybrids allopolyploids. The extent interlocus...