A5057321631- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- Liver physiology and pathology
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Proteoglycans and glycosaminoglycans research
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- Trace Elements in Health
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Iron Metabolism and Disorders
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Cellular Mechanics and Interactions
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Glycosylation and Glycoproteins Research
- Telomeres, Telomerase, and Senescence
- Computational Drug Discovery Methods
- Nuclear Receptors and Signaling
- Complement system in diseases
University of the Basque Country
2015-2025
Innoprot (Spain)
2011
Royal Agricultural University
2006
ABSTRACT This study examined the ability of (i) pure nisin, (ii) nisin-producing Lactococcus lactis strain CHCC5826, and (iii) non-nisin-producing L. CHCH2862 to affect composition intestinal microbiota human flora-associated rats. The presence both strains significantly increased number Bifidobacterium cells in fecal samples during first 8 days but decreased enterococci/streptococci duodenum, ileum, cecum, colon as detected by selective cultivation. No significant changes rat were observed...
<h3>Background</h3> The transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts is a major mechanism for stroma development in metastasis, but their regulatory pathways remain unclear. Transdifferentiated HSCs from fibrotic liver express high levels the fibrillar collagen receptor discoidin domain 2 (DDR2), it unclear if DDR2 plays direct profibrogenic role tumour microenvironment. <h3>Aim</h3> To assess impact on prometastatic HSC-derived myofibroblasts. <h3>Methods</h3>...
Abstract The adipokine apelin has been directly implicated in various physiological processes during embryogenesis and human cancers. Nevertheless, the importance of conversion its precursor proapelin to mature tumorigenesis remains unknown. In this study, we identify Furin as cellular proprotein convertase responsible for cleavage. We explore therapeutic potential targeting cleavage sites metastatic colorectal cancer by introducing apelin-dm, a modified variant resulting from alteration...
Phenotypic transformation of liver sinusoidal endothelial cells is one the most important stages metastasis progression. The miRNA effects on during have not yet been studied. Herein, whole genome analysis expression in these colorectal revealed repressed microRNA‐20a. Importantly, downregulation miR‐20a occurs parallel with upregulation its known protein targets. To restore normal levels cells, we developed chondroitin sulfate‐sorbitan ester nanoparticles conjugated a delivery system that...
Colorectal cancer (CRC) is a major health problem in elderly people because of its high incidence and mortality rate.Despite early screening programs, more than half CRC patients are diagnosed at advanced stages.Fibroblast activation protein-α (FAP) expression cancer-associated fibroblasts (CAFs) has been associated with higher risk metastases poor survival.Here, we have analyzed the immunohistochemical FAP 41 adenoma-carcinoma sequences.In addition, was individually combination β-catenin...
Furin is involved in the endoproteolytic processing of various protein precursors implicated many diseases such as diabetes, obesity, atherosclerosis, cancer, Alzheimer’s disease and viral infection including COVID-19. Recently, cell entry SARS-CoV-2 was found to require sequential cleavage spike glycoprotein (S protein) at S1/S2 S2ʹ sites. The site (PRRAR) can be cleaved by proprotein convertase furin that facilitates membrane fusion spread. Here we summarized recent findings on S...
Proprotein convertases or PCs are known to regulate the malignant phenotype of colon cancer cells by different mechanisms, but their effects on stem (CSCs) have been less widely investigated. Here, we report that expression is altered in CSCs, and inhibition activity reduced CSCs growth, survival, invasion three-dimensional spheroid cultures. In vivo, repression general PC inhibitors α1-PDX, Spn4A, decanoyl-RVKR-chloromethylketone (CMK) significantly tumor levels cell markers LGR5 NANOG...
Abstract Liver metastasis depends on the collagenous microenvironment generated by hepatic sinusoidal cells (SCs). DDR1 is an atypical collagen receptor linked to tumor progression, but whether SCs express and its implication in liver remain unknown. Freshly isolated stellate (HSCs), Kupffer (KCs), endothelial (LSECs), that conform SCs, expressed functional DDR1. HSCs largest amounts. C26 colon carcinoma secretomes increased phosphorylation KCs I. Inhibition of kinase activity DDR1-IN-1 or...
Self-determination theory (SDT) suggests that motivation can interact with resilience and perceived competence. The climate-related characteristics of the classroom influence student motivation. This study aimed to evaluate associations between differentiated theoretical practical teaching, resilience, competence, considering number students per class profiles lecturers. A total 789 participated (mean age = 19.31; SD 3.37) from Psychology, Nursing, Education degrees different Spanish...
The intracellular calcium concentration ([Ca2+]i) modulation plays a key role in the regulation of cellular growth and survival normal cells failure [Ca2+]i homeostasis is involved tumor initiation progression. Here we showed that inhibition Furin by its naturally occurring inhibitor prodomain ppFurin MDA-MB-231 breast cancer resulted enhanced store-operated entry (SOCE) reduced cell malignant phenotype. Expression stable manner melanoma MDA-MB-435 lines inhibits activity as assessed vitro...
DDR1 is a receptor tyrosine kinases for collagen and an adverse prognostic factor in primary metastatic tumors.Despite this, signaling its functional consequences tumor development remain unclear. RT-PCR Western blot show that A375, colon carcinoma HT29 liver SK-HEP human cell lines express phosphorylates response to type I. Chemical inhibition of phosphorylation or mRNA silencing reduced AKT ERK phosphorylation, expression ICAM1 VCAM1, Ki67 secretion MMP9. silenced cells showed adhesion I,...
(1) Background & Aims: The roles of different cells in the tumor microenvironment (TME) are critical to metastatic process. phenotypic transformation liver is one most important stages hepatic metastasis progression colorectal cancer (CRC). Our aim was identify major molecules (i.e., genes, miRNAs and proteins) involved this (2) Methods: We isolated performed whole-genome analysis gene, miRNA, protein expression three types (Ito cells, Kupffer sinusoidal endothelial cells) from TME a...
Abstract Colorectal cancer (CRC) is the second most recurrent type and one of lethal diseases around worldwide. The lethality CRC due to metastasis principal target liver. Although main responsible cell we can not obviate crucial role host organ how cells support progression disease. crosstalk tumor microenvironment with has been deeply studied from cellular point view but regulatory pathways these processes still remain unknown. epigenetic regulation postulated as important process...
Colon cancer is one of the most frequently occurring types cancers in world. Primary tumours are treated very efficiently, but metastatic cases known to have severe outcomes. Therefore, aim present study was obtain a greater understanding transformation primary colon cells into phenotypes. Small changes protein expression provoke phenotype transformation. More sensitive methods detect small variations required. A murine cell line with characteristics early phase created order investigate...
Cancer is a phenomenon broadly related to ageing in various ways such as cell cycle deregulation, metabolic defects or telomerases dysfunction principal processes. Although the tumor main actor cancer progression, it not only element of disease. Cells and matrix surrounding tumor, called microenvironment (TME), play key roles progression. Phenotypic changes TME are indispensable for disease progression few these transformations produced by epigenetic including miRNA dysregulation. In this...