A5081147504- Cholesterol and Lipid Metabolism
- Drug Transport and Resistance Mechanisms
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Histone Deacetylase Inhibitors Research
- Hormonal Regulation and Hypertension
- Pharmacogenetics and Drug Metabolism
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Cancer, Hypoxia, and Metabolism
- Lipid metabolism and biosynthesis
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Cancer, Lipids, and Metabolism
- Nuclear Receptors and Signaling
- Adipokines, Inflammation, and Metabolic Diseases
- Steroid Chemistry and Biochemistry
- Edible Oils Quality and Analysis
- Phytochemicals and Antioxidant Activities
- Metabolism, Diabetes, and Cancer
- Pediatric Hepatobiliary Diseases and Treatments
- Natural product bioactivities and synthesis
University of Milan
2016-2025
Laboratoire de Physique des 2 Infinis Irène Joliot-Curie
2021
Université Paris Cité
2021
Université Paris-Saclay
2021
Institut National de Physique Nucléaire et de Physique des Particules
2021
Centre National de la Recherche Scientifique
2021
Laboratoire de Biochimie
2010
Centro Cardiologico Monzino
2006
Texas A&M University System
2006
Marche Polytechnic University
2006
Chromatin modifications are sensitive to environmental and nutritional stimuli. Abnormalities in epigenetic regulation associated with metabolic disorders such as obesity diabetes that often linked defects oxidative metabolism. Here, we evaluated the potential of class-specific synthetic inhibitors histone deacetylases (HDACs), central chromatin-remodeling enzymes, ameliorate dysfunction. Cultured myotubes primary brown adipocytes treated a class I-specific HDAC inhibitor showed higher...
Bile acid metabolism plays an essential role in cholesterol homeostasis and is critical for the initiation of atherosclerotic disease. However, despite recent advances, molecular mechanisms whereby bile acids regulate gene transcription mammals still need further investigations. Here, we show that suppress (CYP7A1) encoding 7α-hydroxylase, rate-limiting enzyme biosynthesis, also through unusual mechanism not involving nuclear receptor, farnesoid X receptor. By performing cell-based reporter...
NAD+-dependent sirtuin deacetylases have emerged as potential therapeutic targets for treatment of human illnesses such cancer, metabolic, cardiovascular, and neurodegenerative diseases. The benefits modulation by small molecules been demonstrated these In contrast to the discovery inhibitors SIRT1, -2, -3, only activators SIRT1 are known. Here, we rationalized previously unexplored dihydropyridine scaffold in developing ligands, thus prepared a series 1,4-dihydropyridine-based derivatives...
Bile acids regulate the cholesterol 7α-hydroxylase gene (CYP7A1), which encodes rate-limiting enzyme in classical pathway of bile acid synthesis. Here we report a novel mechanism whereby feedback regulatesCYP7A1 transcription through nuclear receptorhepatocyte factor-4 (HNF-4), binds to response element (BARE) at nt −149/−118 relative start site. Using transient transfection assays HepG2 cells with Gal4-HNF-4 fusion proteins, show that chenodeoxycholic (CDCA) dampened transactivation...
The Mediterranean diet reduces the risk of coronary artery disease as a consequence its high content antioxidants, namely, hydroxytyrosol (HT) and oleuropein aglycone (OleA), typical virgin olive oil. Because intercellular vascular cell adhesion molecules (ICAM-1 VCAM-1) E-selectin are crucial for endothelial activation, role phenolic extract from extra oil (OPE), OleA, HT, homovanillyl alcohol (HVA) on surface mRNA expression in human umbilical cells (HUVEC) was evaluated. OPE strongly...
Diabetic peripheral neuropathy (DPN) is characterized by myelin abnormalities; however, the molecular mechanisms underlying such deficits remain obscure. To uncover effects of diabetes on alterations, we have analyzed composition. In a streptozotocin-treated rat model diabetic neuropathy, analysis sciatic nerve lipids revealed that alters myelin's phospholipid, FA, and cholesterol content in pattern can modify membrane fluidity. Reduced expression relevant genes FA biosynthetic pathway...
Abstract White adipose tissue (WAT) can undergo a phenotypic switch, known as browning, in response to environmental stimuli such cold. Post-translational modifications of histones have been shown regulate cellular energy metabolism, but their role white physiology remains incompletely understood. Here we show that histone deacetylase 3 (HDAC3) regulates WAT metabolism and function. Selective ablation Hdac3 fat switches the metabolic signature by activating futile cycle de novo fatty acid...
Helicobacter pylori (H. pylori) is an etiologic factor of peptic ulcer disease and gastric cancer. Virulent strains H. are correlated with the severity gastritis, due to NF-κB activation IL-8 expression at epithelial level. Ellagitannins have been documented for antibacterial anti-inflammatory activities, thus suggesting their potential use in gastritis. Recently, several authors, including our group, demonstrated that tannin-rich extracts from chestnut byproducts, present considered...
A eukaryotic cell-binding domain from the intimin (Int) polypeptide of enteropathogenic Escherichia coli O127 (EPEC) was investigated. Derivatives carboxy-terminal 280-amino-acid domains Int (Int-EPEC280) and homolog invasin (Inv) Yersinia pseudotuberculosis (InvYP280) were fused to E. maltose-binding protein (MBP), expressed, purified. The smallest MBP-IntEPEC fusion that efficiently mediated binding HEp-2 cells, monitored by using purified proteins in fluorescence activated cell sorter...
In vivo studies suggest that the phenolic component contributes to anti-inflammatory and antiatherosclerotic actions of olive oil; however, effects in circulating cells are not fully characterized. Monocytes play a key role inflammation-based diseases by expressing several molecules, including metalloproteinases (MMPs). present study, we investigated oil extract individual compounds on MMP-9 THP-1 cells, human monocyte-like cell line. Olive prevented stimulation expression secretion tumor...
There is evidence that increased availability of taurine enhances the proportion taurine-conjugated bile acids in bile.To explore possibility treatment could also influence hepatic cholesterol and acid metabolism, we fed female hamsters for 1 week measured both biliary lipid content microsomal level rate-limiting enzymes synthesis.In these animals 7a-hydroxylase activity was significantly greater respect to controls (P < 0.05).The total HMG-CoA reductase activity, as well active form,...
Abstract Background Recent data highlighted the role of nuclear receptors in transcriptional regulation limiting enzyme bile acid synthesis, cholesterol 7α‐hydroxylase, cellular and animal models. This study was designed to analyze effects age on 7α‐hydroxylase related receptor expression human livers. Design Surgical liver biopsies were obtained 23 patients requiring operation gastrointestinal tract. mRNA levels co‐activators assayed by quantitative real‐time RT‐PCR. Serum...
The Organic Cation Transporter Novel 1 (OCTN1), also known as SLC22A4, is widely expressed in various human tissues, and involved numerous physiological pathological processes remains. It facilitates the transport of organic cations, zwitterions, with selectivity for positively charged solutes. Ergothioneine, an antioxidant compound, acetylcholine (Ach) are among its substrates. Given lack experimentally solved structures this protein, study aimed at generating a reliable 3D model OCTN1 to...
Abstract Neuroinflammation is defined as a process that includes cellular responses designed to protect the central nervous system from external influences, and it initiates in cases of extreme deviations homeostasis. While serves protective role, excessive immune activation can lead release neurotoxic factors, worsening disease progression. Histone deacetylases (HDACs) have been shown modulate expression inflammatory genes by remodeling chromatin through histone deacetylation. HDAC...