A5061558927- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Toxoplasma gondii Research Studies
- Respiratory Support and Mechanisms
- Nail Diseases and Treatments
- Glycosylation and Glycoproteins Research
- Cytomegalovirus and herpesvirus research
- Alzheimer's disease research and treatments
- Neonatal Respiratory Health Research
- Protein purification and stability
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Coccidia and coccidiosis research
- Diet and metabolism studies
- Chemokine receptors and signaling
- Dermatology and Skin Diseases
- Gut microbiota and health
- Probiotics and Fermented Foods
- Blood properties and coagulation
- Listeria monocytogenes in Food Safety
- Inhalation and Respiratory Drug Delivery
- Parasitic Infections and Diagnostics
Monash University
2015-2024
Centre Hospitalier Universitaire de Martinique
2024
Australian Regenerative Medicine Institute
2016-2023
The University of Sydney
2023
Centre Hospitalier Universitaire de Poitiers
2019
Commonwealth Scientific and Industrial Research Organisation
2008-2014
Materials Science & Engineering
2014
Université d'Angers
1991-2013
Université de Sherbrooke
2006-2013
Université de Bordeaux
2009-2010
Diet and the gut microbiota may underpin numerous human diseases. A major metabolic product of commensal bacteria are short-chain fatty acids (SCFAs) that derive from fermentation dietary fibre. Here we show diets deficient or low in fibre exacerbate colitis development, while very high intake SCFA acetate protects against colitis. SCFAs binding to 'metabolite-sensing' receptors GPR43 GPR109A non-haematopoietic cells mediate these protective effects. The inflammasome pathway has hitherto...
Asthma is prevalent in Western countries, and recent explanations have evoked the actions of gut microbiota. Here we show that feeding mice a high-fibre diet yields distinctive microbiota, which increases levels short-chain fatty acid, acetate. High-fibre or acetate-feeding led to marked suppression allergic airways disease (AAD, model for human asthma), by enhancing T-regulatory cell numbers function. Acetate acetylation at Foxp3 promoter, likely through HDAC9 inhibition. Epigenetic effects...
Recent evidence for the role of laminin in cell adhesion and pathogenesis several bacterial infections has led us to investigate existence receptors this extracellular matrix component Candida albicans. At first, immunofluorescence demonstrated presence laminin-binding sites at surface germ tubes. Electron microscopy confirmed result permitted precise localization binding on outermost fibrillar layer tube wall. By using 125I-radiolabeled laminin, was shown be saturable specific, hence...
Butyrate is a short‐chain fatty acid derived from the metabolism of indigestible carbohydrates by gut microbiota. contributes to homeostasis, but it may also control inflammatory responses and host physiology in other tissues. inhibits histone deacetylases, thereby affecting gene transcription, signals through metabolite‐sensing G protein receptor (GPR)109a. We produced an mAb mouse GPR109a found high expression on podocytes kidney. Wild‐type Gpr109a –/– mice were induced develop nephropathy...
Autoimmune diseases are characterized by a breakdown of immune tolerance partly due to environmental factors. The short-chain fatty acid acetate, derived mostly from gut microbial fermentation dietary fiber, promotes antiinflammatory Tregs and protects mice type 1 diabetes, colitis, allergies. Here, we show that the effects acetate extend another important subset involved in tolerance, IL-10–producing regulatory B cells (B10 cells). Acetate directly promoted B10 cell differentiation mouse...
Germ tubes of Candida albicans produced an additional fibrillar surface layer responsible for enhanced adherence to plastic. The correlation between germination C. and germ a plastic matrix led us consider the existence tube-specific adhesive components involved in attachment process. Using concanavalin A-sensitized latex microspheres, we first detected extracellular molecules on after removal adherent tubes. Electron microscopy confirmed that fibrils tube cell-substratum interconnections...
Abstract The noninvasive imaging of atherosclerotic plaques at an early stage atherogenesis remains a major challenge for the evaluation pathologic state patients high risk acute coronary syndromes. Recent studies have emphasized importance platelet–endothelial cell interactions in atherosclerosis‐prone arteries stages, and prominent role P‐selectin initial loose contact between platelets diseased vessel walls. A specific MR contrast agent was developed here targeting, with affinity,...
Ulcerative colitis is an inflammatory disease of the colon that associated with colonic neutrophil accumulation. Recent evidence indicates diet alters composition gut microbiota and influences host–pathogen interactions. Specifically, bacterial fermentation dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various diseases. However, effect deficiency on key initial steps inflammation, such as leukocyte–endothelial cell...
Most neurodegenerative disorders, such as Alzheimer's (AD), Parkinson's, Huntington's and Creutzfeldt–Jakob disease, are characterised by the accumulation of insoluble filamentous aggregates known amyloid. These pathologies share common pathways involving protein aggregation which can lead to fibril formation amyloid plaques. The 4 kDa Aβ peptide (39–43 amino acids) derived from proteolysis precursor is currently a validated target for therapy in AD. Both active passive immunisation studies...
The antigenic composition of Candida albicans is very complex. In order to study the relationship between blastoconidia and germ tubes C. albicans, we produced several monoclonal antibodies analyzed their reactivity against cell wall antigens either in intact cells or treated with dithiothreitol. Overall, four types were found. Monoclonal 3D9 15C9 stained only when tested by indirect immunofluorescence. However, they showed a different immunoblotting. antibody reacted molecular masses >...
The genes encoding the Msp l restriction modification system, which recognizes sequence 5′CCGG, have been cloned into pUC9. Selection was based on expression of methylase gene renders plasmid DNA insensitive to cleavage in vitro . Initially, an insert 15kb obtained which, upon subcloning, yielded a 3 kb Eco RI Hind III insert, carrying for both and enzyme. This has sequenced. Based sequence, together with appropriate subclones, it is shown that two are transcribed divergently polypeptide 418...
Polymer hydrogel capsules comprised of poly(methacrylic acid) chains and cross-linked via disulfide linkages were investigated for their cytotoxicity mechanism internalization in a variety mammalian cells. The internalized by all the tested cell lines which differed morphology function over short to medium term (24 h) revealed no reduction viability metabolic activity capsule uptake was analyzed using inhibitors various cellular entry pathways. Of these, blocking clathrin-mediated...
The chemokine receptor CCR6 marks subsets of T cells and innate lymphoid that produce IL-17 IL-22, as such may play a role in the recruitment these to certain inflammatory sites. However, precise has been controversial, part because no effective monoclonal antibody (mAb) inhibitors against this exist for use mouse models inflammation. We circumvented problem using transgenic mice expressing human (hCCR6) under control its native promoter (hCCR6-Tg/mCCR6-/-). also developed fully humanized...
pH sensing by GPR65 regulates various inflammatory conditions, but its role in skin remains unknown. In this study, we performed a phenome-wide association study and report that the T allele of GPR65-intronic single-nucleotide polymorphism rs8005161, which reduces signaling, showed significant with atopic dermatitis, addition to bowel diseases asthma, as previously reported. Consistent genetic humans, show deficiency mice resulted markedly exacerbated disease MC903 experimental model...
Atopic dermatitis (AD) is a chronic, relapsing, and extremely pruritic inflammatory skin disease with particular impact on children. AD pathogenesis not yet fully understood, there no curative treatment for this disease. Therefore, several genetically or chemically-induced mouse models have been developed. These preclinical are an indispensable research tool studying evaluating the efficacy of new candidate therapeutics. A commonly used model has developed using topical application...
The monoclonal antibody (MAb) AF1 recognizes an oligosaccharide epitope present on highly immunogenic and immunomodulatory mannoproteins (MP) of Candida albicans. expression this (AF1-MP) during experimental candidal vaginitis was studied in two strains C. albicans (3153 CA-2) which were equally vaginopathic but differed the mode hypha formation vagina. In both strains, immunofluorescence vaginal samples, taken 1 h after challenge, revealed intense, MAb AF1-specific labelling yeast cells....
The binding of human fibrinogen to the pathogenic aspergilli was investigated in vitro by different procedures using either solution or fixed, insolubilized fibrinogen. Binding detected at surface hyphae and conidia an immunofluorescence assay. Ultrastructural localization sites visualized with fibrinogen-sensitized gold particles. labelling restricted outer cell wall layer 'smooth' walled conidia. Quantitative analysis carried out 125I-labelled on 33 species belonging groups (opportunistic...
Objective Antibody‐mediated autoimmunity involves cognate interactions between self‐reactive T cells and B during germinal center (GC) reactions. The aim of this study was to determine the role essential follicular helper (Tfh) cell molecules (CXCR5, signaling lymphocytic activation molecule–associated protein) on autoreactive CD4+ certain environmental influences that may GC‐driven autoantibody production arthritis development. Methods We transferred from KRN‐Tg mice into recipient mice,...
Staphylococcus aureus causes necrotizing pneumonia by secreting toxins such as leukocidins that target front-line immune cells. The mechanism which kill innate cells and trigger inflammation during S. lung infection, however, remains unresolved. Here, we explored human-induced pluripotent stem cell-derived macrophages (hiPSC-dMs) to study the interaction of Panton-Valentine leukocidin (PVL) LukAB with macrophages, are initial targets invasion. hiPSC-dMs were susceptible PVL both triggered...