A5040355211- Viral Infections and Vectors
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Antibiotic Resistance in Bacteria
- Viral Infections and Outbreaks Research
- Salmonella and Campylobacter epidemiology
- Leptospirosis research and findings
- Yeasts and Rust Fungi Studies
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Brucella: diagnosis, epidemiology, treatment
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Burkholderia infections and melioidosis
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- Vibrio bacteria research studies
- Virology and Viral Diseases
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
Defense Health Agency
2022
United States Naval Medical Research Unit SOUTH
2007-2020
Naval Medical Research Unit EURAFCENT
2005-2017
Naval Medical Research Command
2006-2016
Northern Arizona University
2009
Centers for Disease Control and Prevention
2009
Ministry of Health
2009
National Center for Disease Control and Public Health
2009
Technology Management Company (United States)
2009
Walter Reed Army Institute of Research
2009
A survey of 179 animals (black rats, dogs, sheep, buffaloes, cattle, donkeys, weasels, and cats) for Leptospira infection was conducted in Mahalla City (Lower Egypt). Blood, urine, kidney were collected tested by culture, microscopic agglutination test (MAT), and/or polymerase chain reaction (PCR). Among 26% positive PCR, including 7% that also culture L. interrogans serovars Grippotyphosa, Pyrogenes, Icterohaemorrhagiae. borpetersenii serovar Polonica isolated the first time Egypt three...
Brucellosis poses a significant public health problem in Mediterranean countries, including Egypt. Treatment of this disease is often empirical due to limited information on the antibiotic susceptibility profiles Brucella spp. region world. The aim study was determine blood isolates Egypt, country endemic for brucellosis. were identified from cultures acute febrile illness (AFI) patients presenting network infectious hospitals 1999–2007. Minimum inhibitory concentrations determined...
Little is known about the role of viral respiratory pathogens in etiology, seasonality or severity severe acute infections (SARI) Eastern Mediterranean Region.Sentinel surveillance for SARI was conducted from December 2007 through February 2014 at 20 hospitals Egypt, Jordan, Oman, Qatar and Yemen. Nasopharyngeal oropharyngeal swabs were collected hospitalized patients meeting case definitions analyzed infection with influenza, syncytial virus (RSV), adenovirus (AdV), human metapneumovirus...
Of 49 workers at a Djiboutian abattoir, eight (16%, 95% confidence interval [CI]: 9-29) were seropositive against spotted fever group rickettsiae (SFGR), two (4%, CI: 1-14) typhus rickettsiae, and three (6%, 2-17) orientiae. One worker (9%, 2-38) seroconverted orientiae during the study period. This is first evidence of exposure in Horn Africa. SFGR also identified by polymerase chain reaction 32 189 (11%, 8-15) tick pools from 26 72 (36%) cattle. Twenty-five (8%, 6-12) positive for...
Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority all viruses thought to be undiscovered. Accordingly, recent studies have shown that discovered in insects very divergent from known pathogens and them lack appropriate reference sequences the public databases. Given likely still undiscovered, environ mental sampling stands provide much needed samples as well genetic for comparison. In this study, we sought...
Following the appearance of influenza A/H5 virus infection in several wild and domestic bird species Republic Azerbaijan February 2006, two clusters potential human avian due to A/H5N1 (HAI) cases were detected reported by Ministry Health (MoH) World Organization (WHO) Regional Office for Europe during first weeks March 2006. On 15 WHO led an international team, including control, clinical management, epidemiology, laboratory, communications experts, support MoH investigation response...
Although antimicrobial therapy of leptospirosis has been studied in a few randomized controlled clinical studies, those studies were limited to specific regions the world and have characterized infecting strains. A broth microdilution technique for assessment antibiotic susceptibility developed at Brooke Army Medical Center. In present study, we assessed susceptibilities 13 Leptospira isolates (including recent isolates) from Egypt, Thailand, Nicaragua, Hawaii agents. Ampicillin, cefepime,...
In this study, we evaluated a recently developed multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) method for the molecular typing of Mycoplasma pneumoniae. The is based on GeneScan five VNTR loci throughout genome which define specific genotype number tandem repeats within each locus. A retrospective 154 M. pneumoniae clinical isolates collected over last 50 years and limited (n = 4) pneumoniae-positive primary specimens acquired by CDC was performed using MLVA. Eighteen...
Background. Lateral flow immunoassays (LFIs) are point-of-care diagnostic assays that designed for single use outside a formal laboratory, with in-home pregnancy tests the best-known example of these tests. Although LFI has some limitations over more-complex immunoassay procedures, such as reduced sensitivity and potential false-positive results when using complex sample matrices, assay benefits rapid time to result ease use. These make it an attractive option obtaining in austere...
We report the occurrence of concurrent infections with multiple acute febrile illness (AFI) pathogens during an ongoing prospective laboratory-based surveillance in four infectious disease hospitals urban and rural areas Egypt from June 2005 to August 2006. Patients were screened for Leptospira, Rickettsia typhi, Brucella, or Salmonella enterica serogroup Typhi by various methods including serology, culture, PCR. One hundred eighty-seven 1,510 patients (12.4%) evaluated had supporting...
The epidemiology of serogroup X meningococcal meningitis in Africa is unknown. During a meningococcus outbreak Kenya, case finding involved record review at health facilities and interviews with workers community leaders West Pokot district. An age- location-matched case-control study for risk factors was done. From December 2005 to April 2006, 82 suspect cases were reported; the epidemic threshold surpassed within two administrative divisions. Most (58%) 5–24 years old; case-fatality ratio...
Typhoid fever is endemic in many parts of the world and represents a major cause acute febrile illness (AFI). Rapid accurate laboratory methods for diagnosis this disease are needed both patient care surveillance situations.Serum samples were collected from AFI patients used to evaluate performance newly developed ELISA assay that uses mixture somatic flagellar antigens detect total antibody response against Salmonella enterica subspecies serovar Typhi (S. Typhi) infection. The levels Ig...
Klebsiella pneumoniae outbreaks possessing extended-spectrum β-lactamase- (ESBL) mediated resistance to third-generation cephalosporins have increased significantly in hospital and community settings worldwide. The study objective was characterize prevalent genetic determinants of TEM, SHV CTX-M types ESBL activity K. isolates from Egypt. Sixty five ESBL-producing strains, isolated nosocomial community-acquired infections 10 Egyptian University hospitals (2000–2003), were confirmed with...
Brucella species are highly monomorphic, with minimal genetic variation among species, hindering the development of reliable subtyping tools for epidemiologic and phylogenetic analyses. Our objective was to compare two distinct multiple-locus variable-number tandem-repeat analysis (MLVA) methods on a collection 101 melitensis isolates from sporadic human cases brucellosis in Egypt (n = 83), Qatar 17), Libya 1). A gel-based MLVA technique, MLVA-15(IGM), compared an automated capillary...
Brucellosis is a worldwide zoonotic disease that often requires serology for diagnosis. The serum agglutination test the gold standard assay, but ELISAs are used by many laboratories. Many commercial available, few studies have compared their performance. This study ability of four commercially available ELISA kits (from Bio-Quant, Immuno-Biological Laboratories – America, Vircell and Euroimmun) to diagnose brucellosis in patients from Egypt USA. sensitivities all tested, except kit, were...
An outbreak of acute febrile illness was reported among Somali pastoralists in remote, arid Northeast Kenya, where drinking raw milk is common. Blood specimens from 12 patients, collected mostly the late convalescent phase, were tested for viral, bacterial, and parasitic pathogens. All negative viral typhoid serology. Nine patients had Brucella antibodies present by at least one tests, four whom evidence suggestive infection reference serologic microscopic agglutination test. Three positive...
Objectives: To determine the antimicrobial susceptibility and serotype distribution of 205 isolates Streptococcus pneumoniae, collected from CSF meningitis patients identified between 1998–2003, during sentinel surveillance in Egypt.
Given the protean manifestations of leptospirosis, adequate laboratory support for diagnosis is necessary. Traditionally, gold standard microscopic agglutination test (MAT) using a panel Leptospira isolates representing broad range serogroups and serovars. It has been proposed that screening with serovars circulating in region would enhance performance. We assessed diagnostic usefulness MAT both regionally obtained clinical specific recovered from tested patients. Serum 41 acute febrile...
We analyzed highly pathogenic avian influenza A(H5N1) viruses isolated from humans infected in Egypt during 2007-2011. All evolved the lineage of subtype H5N1 introduced into 2006; we found minimal evidence reassortment and no exotic introductions. The hemagglutinin genes 2011 formed a monophyletic group within clade 2.2.1 that also included human 2009 2010 contemporary poultry; this finding is consistent with zoonotic transmission. Although molecular markers suggestive decreased...
Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools necessary to aid in curbing spread antibiotic resistance. In this study, we describe use Antimicrobial Resistance Determinant Microarray (ARDM) targets 239 unique genes confer 12 classes compounds, quaternary amines and streptothricin for determination multidrug (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well...