A5028692982- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Legionella and Acanthamoeba research
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Antifungal resistance and susceptibility
- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Viral gastroenteritis research and epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Biosensors and Analytical Detection
- Neonatal and Maternal Infections
- Cervical Cancer and HPV Research
- Water Treatment and Disinfection
- Influenza Virus Research Studies
- SARS-CoV-2 detection and testing
- Child Nutrition and Water Access
- Autopsy Techniques and Outcomes
- Urinary Tract Infections Management
- Global Maternal and Child Health
- Vibrio bacteria research studies
- Bacteriophages and microbial interactions
- Viral Infections and Outbreaks Research
- Antibiotic Resistance in Bacteria
- Immunotherapy and Immune Responses
Centers for Disease Control and Prevention
2015-2024
National Center for Immunization and Respiratory Diseases
2013-2024
Health Net
2023
Leidos (United States)
2021
Battelle
2021
Center for Surveillance, Epidemiology, and Laboratory Services
2021
Hyattsville Community Development Corporation
2020
CDC Foundation
2019
Ministry of Health and Welfare
2019
Virginia Department of Health
2019
Community-acquired pneumonia is a leading infectious cause of hospitalization and death among U.S. adults. Incidence estimates confirmed radiographically with the use current laboratory diagnostic tests are needed.
Incidence estimates of hospitalizations for community-acquired pneumonia among children in the United States that are based on prospective data collection limited. Updated has been confirmed radiographically and with use current laboratory diagnostic tests needed.We conducted active population-based surveillance requiring hospitalization younger than 18 years age three hospitals Memphis, Nashville, Salt Lake City. We excluded recent or severe immunosuppression. Blood respiratory specimens...
The large and growing number of viral bacterial pathogens responsible for respiratory infections poses a challenge laboratories seeking to provide rapid comprehensive pathogen identification. We evaluated novel application the TaqMan low-density array (TLDA) cards real-time PCR detection 21 respiratory-pathogen targets. performance TLDA was compared that individual (IRTP) assays with same primers probes using (i) nucleic acids extracted from strains 66 closely related viruses bacteria (ii)...
The epidemiology of Mycoplasma pneumoniae (Mp) among US children (<18 years) hospitalized with community-acquired pneumonia (CAP) is poorly understood.In the Etiology Pneumonia in Community study, we prospectively enrolled 2254 radiographically confirmed from January 2010-June 2012 and tested nasopharyngeal/oropharyngeal swabs for Mp using real-time polymerase chain reaction (PCR). Clinical epidemiological features PCR-positive -negative were compared logistic regression. Macrolide...
More than 500 000 neonatal deaths per year result from possible serious bacterial infections (pSBIs), but the causes are largely unknown. We investigated incidence of community-acquired caused by specific organisms among neonates in south Asia.From 2011 to 2014, we identified babies through population-based pregnancy surveillance at five sites Bangladesh, India, and Pakistan. Babies were visited home community health workers up ten times age 0 59 days. Illness meeting WHO definition pSBI...
Abstract A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of SARS-CoV genome, could discriminate from other human animal coronaviruses with a potential detection limit <10 genomic copies per reaction. RT-PCR more sensitive than conventional or culture isolation proved suitable...
Mycoplasma pneumoniae is a significant cause of community-acquired pneumonia, which often empirically treated with macrolides or azalides such as erythromycin azithromycin. Recent studies have discovered the existence macrolide-resistant strains within population that been mapped to mutations domain V region 23S rRNA gene. Currently, identification these resistant relies on time-consuming and labor-intensive procedures restriction fragment length polymorphism, MIC studies, sequence analysis....
ABSTRACT The genus Bartonella includes numerous species with varied host associations, including several that infect humans. Development of a molecular diagnostic method capable detecting the diverse repertoire while maintaining specificity has been challenge. We developed novel real-time PCR assay targeting 301-bp region ssrA gene and demonstrated specific amplification in over 30 species, subspecies, strains. Subsequent analysis sequences was sufficient to discriminate provided...
Background Few comprehensive data exist on disease incidence for specific etiologies of acute respiratory illness (ARI) in older children and adults Africa. Methodology/Principal Findings From March 1, 2007, to February 28, 2010, among a surveillance population 21,420 persons >5 years old rural western Kenya, we collected blood culture malaria smears, nasopharyngeal oropharyngeal swabs quantitative real-time PCR ten viruses three atypical bacteria, urine pneumococcal antigen testing...
BackgroundSub-Saharan Africa and south Asia contributed 81% of 5·9 million under-5 deaths 77% 2·6 stillbirths worldwide in 2015. Vital registration verbal autopsy data are mainstays for the estimation leading causes death, but both non-specific focus on a single underlying cause. We aimed to provide granular contributory death stillborn fetuses deceased neonates children younger than 5 years, inform child mortality prevention efforts.MethodsThe Child Health Mortality Prevention Surveillance...
ABSTRACT Mycoplasma pneumoniae is a leading cause of respiratory infections, including community-acquired pneumonia (CAP). Currently, pathogen-specific testing not routinely performed in the primary care setting, and United States lacks systematic surveillance program for M. . Documentation individual cases clusters typically occurs only when severe illness and/or failure to improve with empirical antibiotic therapy observed. Outbreaks, some lasting extended periods involving large number...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019, and the outbreak rapidly evolved into current disease pandemic. SARS-CoV-2 is a virus that causes symptoms similar to those caused by influenza A B viruses. On July 2, 2020, US Food Drug Administration granted emergency use authorization for vitro diagnostic of Influenza Multiplex Assay. This assay detects at 102.0, 102.2, 100.3 50% tissue culture or egg infectious dose, as few 5 RNA copies/reaction....
Mycoplasma pneumoniae continues to be a significant cause of community-acquired pneumonia (CAP). A more definitive methodology for reliable detection M. is needed identify outbreaks and prevent potentially fatal extrapulmonary complications.We analyzed 2 CAP due pneumoniae. Nasopharyngeal and/or oropharyngeal swab specimens serum samples were obtained from persons with clinically defined cases, household contacts, asymptomatic individuals. Real-time polymerase chain reaction (PCR) was...
ABSTRACT We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents superior target for detecting M. DNA in clinical specimens, although use multiple targets may increase testing confidence.
SUMMARY Although pneumonia is a leading cause of death from infectious disease worldwide, comprehensive information about its causes and incidence in low- middle-income countries lacking. Active surveillance hospitalized patients with ongoing Thailand. Consenting are tested for seven bacterial 14 viral respiratory pathogens by PCR culture on nasopharyngeal swab specimens, serology acute/convalescent sera, sputum smears antigen detection tests urine. Between September 2003 December 2005,...
ABSTRACT We developed a single-tube multiplex real-time PCR assay capable of simultaneously detecting and discriminating Legionella spp., pneumophila , serogroup 1 in primary specimens. Evaluation 21 clinical specimens 115 isolates demonstrated this to be rapid, high-throughput diagnostic test with 100% specificity that may aid during legionellosis outbreaks epidemiologic investigations.
Identification of etiology remains a significant challenge in the diagnosis infectious diseases, particularly resource-poor settings. Viral, bacterial, and fungal pathogens, as well parasites, play role for many syndromes, optimizing single diagnostic system to detect range pathogens is challenging. The TaqMan Array Card (TAC) multiple-pathogen detection method that has previously been identified valuable technique determining infections holds promise expanded use clinical microbiology...
Specimens collected after antibiotic exposure may reduce culture-based bacterial detections. The impact on culture-independent diagnostic tests is unclear. We assessed the effect of both these test results among patients hospitalized with community-acquired pneumonia (CAP).Culture-based testing included blood cultures and high-quality sputum or endotracheal tube (ET) aspirates; urinary antigen (adults) for Streptococcus pneumoniae Legionella pneumophila polymerase chain reaction (PCR)...
Few comprehensive data exist on the etiology of severe acute respiratory illness (SARI) among African children.From March 1, 2007 to February 28, 2010, we collected blood for culture and nasopharyngeal oropharyngeal swabs real-time quantitative polymerase chain reaction 10 viruses 3 atypical bacteria children aged <5 years with SARI, defined as World Health Organization-classified or very pneumonia oxygen saturation <90%, who visited a clinic in rural western Kenya. We from controls without...
BACKGROUND: Stevens-Johnson syndrome (SJS) is an uncommon, sporadic disease and outbreaks are rare. In November 2013, outbreak of SJS was identified at Children’s Hospital Colorado. METHODS: Outbreak cases were children aged 5–21 with a discharge diagnosis admitted from September 1 to 30, 2013. Medical charts reviewed using standardized data collection forms. Respiratory specimens tested for viruses Mycoplasma pneumoniae (Mp) by polymerase chain reaction (PCR). We conducted separate 4-year...
Since the establishment of sequence-based typing as gold standard for DNA-based Legionella pneumophila, laboratory at Centers Disease Control and Prevention (CDC) has conducted routine (SBT) analysis all incoming L. pneumophila serogroup 1 (Lp1) isolates to identify potential links between cases better understand genetic diversity clonal expansion among bacteria. Retrospective genotyping Lp1 from sporadic Legionnaires' disease (LD) outbreaks deposited into CDC reference collection since 1982...