The prevalence of non-alcoholic fatty liver disease (NAFLD) is increasing annually, and emerging evidence suggests that the gut microbiota plays a causative role in development NAFLD. However, NAFLD remains unclear warrants further investigation. Thus, C57BL/6J mice were fed high-fat diet (HFD), we found HFD significantly induced obesity increased accumulation intrahepatic lipids, along with alterations serum biochemical parameters. Moreover, it was observed also impaired barrier integrity....

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disease characterized by persistent anovulation and hyperandrogenism, affecting approximately 8–10% of women childbearing age occupying an important position in the etiology infertility. There increasing evidence that long non-coding RNAs (lncRNAs) are involved development PCOS, but potential regulatory mechanism still unclear. This study performed high-throughput lncRNA sequencing follicular fluid exosomes non-PCOS...

The placenta is an organ with extraordinary phenotypic diversity in eutherian mammals. Recent evidence suggests that numerous human placental enhancers are evolved from lineage-specific insertions of endogenous retroviruses (ERVs), yet the transcription factors (TFs) underlying their regulation remain largely elusive. Here, by first focusing on MER41, a primate-specific ERV family previously linked to and innate immunity, we uncover binding motifs multiple crucial trophoblast TFs (GATA2/3,...

Melatonin is an important endogenous hormone that shows antioxidant functions and pleiotropic effects, playing a crucial role in animal reproduction. Ovary granulosa cells (GCs) surround the oocyte, which play regulating oocytes development. Deoxynivalenol (DON) common fusarium mycotoxin contaminant of feedstuff food, posing serious threat to human reproductive systems. Herein, murine ovary GCs were studied as reproduction cell model, aimed assess protective effect melatonin on DON-induced...

Novel binary composite urea–formaldehyde/poly(butylene succinate) (UF/PBS) and its ternary nanocomposite UF/PBS/potassium dihydrogen phosphate (MKP) were prepared by a simple scalable reactive extrusion approach using methylolurea (MU), PBS, MKP as the raw materials. The results show that MUs react melt polycondensation to form UFs with different polymerization degrees at high temperature in extruder, giving two polymer components molecular segment-scale mixing composites. Meanwhile, MKPs...

Pigs can be colonized with Salmonella enterica and become established carriers. However, the mechanisms of host’s response to infection are largely unclear. This study was constructed model in vitro using porcine intestinal epithelial cells (IPEC-J2). Transcriptome profiling IPEC-J2 carried out characterize effect lipopolysaccharide (LPS) treatment, which LPS-induced inflammation a positive control. At first, increased cell apoptosis rate induced an IPEC-J2. Then, up-regulated genes were...

Escherichia coli F18 is a common conditional pathogen that associated with variety of infections in humans and animals. LncRNAs have emerged as critical players infection, but their role the resistance host to bacterial diarrhea remains unknown. Here, we used piglets animal model identified an antisense lncRNA termed FUT3-AS1 regulator related E . infection by RNA sequencing. Downregulation expression contributed enhancement IPEC-J2 cells. knockdown reduced FUT3 via decreasing H4K16ac level...

Polycystic ovary syndrome (PCOS) is a common reproductive endocrine disease that affects women of age. It also significant cause infertility. Circular RNAs have been found to crucial role in the development and progression system diseases. In this study, we focused on circ_BECN1 aimed investigate its mechanism PCOS, providing foundation for early diagnosis treatment condition. Our findings revealed an upregulation expression ovarian granulosa cells (GCs) PCOS patients. Additionally,...

Deoxynivalenol (DON) is a common environmental toxin that secreted by fusarium fungi frequently contaminates feedstuff and food. While the detrimental effects of DON on human animal reproductive systems have been well recognized, underlying mechanism remains poorly understood. Ovarian granulosa cells (GCs), which surround oocytes, are crucial for regulating oocyte development, mainly through secretion hormones such as estrogen progesterone. Using an in vitro model murine GCs, we...

Deoxynivalenol (DON) is a type of mycotoxin that threatens human and livestock health. Right open reading frame kinase 2 (RIOK2) has pivotal function in ribosome maturation cell cycle progression. This study aims to clarify the role RIOK2 gene DON-induced cytotoxicity regulation porcine intestinal epithelial cells (IPEC-J2). Cell viability assay flow cytometry showed knockdown inhibited proliferation induced apoptosis, arrest, oxidative stress IPEC-J2. Then, transcriptome profiling...

Abstract Background: Deoxynivalenol (DON) is a common fusarium mycotoxin contaminant of food and feedstuff, posing serious threat to human animal reproductive system by inducing oxidative stress inflammation. The antioxidant melatonin participates in immunoregulation, playing crucial role development. So, our present study aimed explore DON-induced toxicity murine ovary granulosa cells (GCs), assess the potential protective effect melatonin. Results: Melatonin inhibits GCs decreased...

Post-weaning diarrhea (PWD) is frequently associated with E. coli F18 infections in piglets. However, the underlying molecular mechanism concerning resistance of local weaned piglets China not clearly understood. In present study, by a comparative analysis transcriptome, a-1,3-fucosyltransferase (FUT3) was evaluated as key candidate correlated to Sutai and Meishan Functional demonstrated that FUT3 acts positive regulator susceptibility newly food accustomed core promoter at −500–(−206) bp...

The Sec1 gene encodes galactose 2-L-fucosyltransferase, whereas expression during development of the mouse and its effect on composition gut microbiota have rarely been reported. In this study, we examined development, constructed knockout mice, sequenced their microbial composition. It was found that expressed at different stages development. mice significantly higher intraperitoneal fat accumulation body weight than wild-type mice. Analysis in revealed phylum level, Bacteroidetes accounted...

Transposable elements (TEs) are key contributors to genetic novelty. Despite increasing evidence of their importance, roles in shaping the regulatory landscape diverse immune cell populations remain largely unclear. Using single-cell multiome data from human peripheral blood mononuclear cells, we annotated cell-specific cis-regulatory for major and identified a highly signature overrepresented TE families. Focusing on monocytes that bear fast-evolving transcriptomes, found high proportions...

Like other mammalian species, the pig genome is abundant with transposable elements (TEs). The importance of TEs for three-dimensional (3D) chromatin organization has been observed in species like human and mouse, yet current understanding about absent. Here, we investigated contribution 3D three tissues, focusing on spleen which crucial both adaptive innate immunity. We identified dozens TE families overrepresented CTCF binding sites, including LTR22_SS, LTR15_SS LTR16_SSc are pig-specific...

Abstract Pig is a widely-used for biomedical studies and xenotransplantation, yet its immune cell heterogeneity remains poorly characterized. Here, we generate single-cell transcriptomes chromatin maps the Peripheral Blood Mononuclear Cells (PBMCs) of two porcine breeds with distinct traits. Dozens types are annotated, γδ T lymphocytes making up one-fifth all PBMCs. Both cellular composition gene expression abundance underlie trait variations across breeds. Transcription factors associated...

Abstract The placenta is an organ with extraordinary phenotypic diversity in eutherian mammals. Recent evidence suggests that numerous human placental enhancers are evolved from lineage-specific insertions of endogenous retroviruses (ERVs), yet the transcription factors (TFs) underlying their regulation remain largely elusive. Here, by first focusing on MER41, a primate-specific ERV family previously linked to and innate immunity, we uncover binding motifs multiple crucial trophoblast TFs...