A5078965269- Lipid Membrane Structure and Behavior
- Force Microscopy Techniques and Applications
- Blood properties and coagulation
- Proteins in Food Systems
- Nanopore and Nanochannel Transport Studies
- Spectroscopy and Quantum Chemical Studies
- Bone and Dental Protein Studies
- Polymer Surface Interaction Studies
- Platelet Disorders and Treatments
- Advanced Fluorescence Microscopy Techniques
- Adhesion, Friction, and Surface Interactions
- Polysaccharides Composition and Applications
- Photosynthetic Processes and Mechanisms
- Block Copolymer Self-Assembly
- Electrochemical Analysis and Applications
- Nanocluster Synthesis and Applications
- Dental materials and restorations
- Microfluidic and Bio-sensing Technologies
- Fluoride Effects and Removal
- Rheology and Fluid Dynamics Studies
- Planetary Science and Exploration
- Lubricants and Their Additives
- Astro and Planetary Science
- Infant Nutrition and Health
- Crystallization and Solubility Studies
University of Leeds
2016-2025
Institute of Structural and Molecular Biology
2006-2024
University of Helsinki
2012
Goethe University Frankfurt
2012
University of Illinois Urbana-Champaign
2006
Leeds Dental Hospital
2003-2004
University of Nottingham
2001-2002
University of California, San Diego
2001
King's College London
2001
Medical Research Council
2001
The established hypothesis for the increase on erythrocyte aggregation associated with a higher incidence of cardiovascular pathologies is based an plasma adhesion proteins concentration, particularly fibrinogen. Fibrinogen-induced has been considered to be caused by its nonspecific binding membranes. In contrast, platelets are known have fibrinogen integrin receptor expressed membrane surface (the glycoprotein complex αIIbβ3). We demonstrate, force spectroscopy measurements using atomic...
Hemostasis requires conversion of fibrinogen to fibrin fibers that generate a characteristic network, interact with blood cells, and initiate tissue repair. The network is porous highly permeable, but the spatial arrangement external clot face unknown. Here we show transitioned blood-air interface through Langmuir film formation, producing protective confining clots in human mouse models. We demonstrated only required for formation film, it occurred vitro vivo. connected underlying tethering...
Polybia-MP1 (MP1) is a bioactive host-defense peptide with known anticancer properties. Its activity attributed to excess serine (phosphatidylserine (PS)) on the outer leaflet of cancer cells. Recently, higher quantities phosphatidylethanolamine (PE) were also found at these cells' surface. We investigate interaction MP1 model membranes in presence and absence POPS (PS) DOPE understand role lipid composition MP1's characteristics. Indeed we find that PS lipids significantly enhance bound...
Abstract 2D metal nanomaterials offer exciting prospects in terms of their properties and functions. However, the ambient aqueous synthesis atomically‐thin, metallic represents a significant challenge. Herein, freestanding atomically‐thin gold nanosheets with thickness only 0.47 nm (two atomic layers thick) are synthesized via one‐step approach at 20 °C, using methyl orange as confining agent. Owing to high surface‐area‐to‐volume ratio, abundance unsaturated atoms exposed on surface large...
With the resource-intensive meat industry accounting for over 50% of food-linked emissions, plant protein consumption is an inevitable need hour. Despite its significance, key barrier to adoption proteins their astringent off-sensation, typically associated with high friction and consequently poor lubrication performance. Herein, we demonstrate that by transforming into physically cross-linked microgels, it possible improve lubricity remarkably, dependent on volume fractions, as evidenced...
Understanding the functional significance of coordinate expression specific corepressors and DNA-binding transcription factors remains a critical question in mammalian development. During development pituitary gland, two highly related paired -like homeodomain factors, repressor, Hesx1/Rpx an activator, Prop-1, are expressed sequential, overlapping temporal patterns. Here we show that while repressive actions may be required for initial organ commitment, progression beyond appearance first...
An electrode surface is presented that enables the characterization of redox-active membrane enzymes in a native-like environment. ubiquinol oxidase from Escherichia coli, cytochrome bo3 (cbo3), has been co-immobilized into tethered bilayer lipid membranes (tBLMs). The tBLM formed on gold surfaces functionalized with cholesterol tethers which insert lower leaflet membrane. planar architecture by self-assembly proteoliposomes, and its structure characterized plasmon resonance (SPR),...
Aβ (amyloid-β peptide) assembles to form amyloid fibres that accumulate in senile plaques associated with AD (Alzheimer's disease). The major constituent, a 42-residue Aβ, has the propensity assemble and soluble potentially cytotoxic oligomers, as well ordered stable fibres. It is widely believed cytotoxicity result of formation transient oligomers. This observed toxicity may be ability oligomers associate cause permeation lipid membranes. In present study, we have investigated oligomeric...
Associative electrostatic interactions between two oppositely charged globular proteins, lactoferrin (LF) and pea protein isolate (PPI), the latter being a mixture of vicilin, legumin, convicilin, was studied with specific PPI/LF molar ratio at room temperature. Structural aspects complexes probed different length scales were investigated as function pH by means complementary techniques, namely, dynamic light scattering, small-angle X-ray scattering (SAXS), turbidity measurements, atomic...
Auxetic materials have negative Poisson's ratios and so expand rather than contract in one or several direction(s) perpendicular to applied extensions. The auxetics community has long sought synthetic molecular - non-porous, inherently auxetic which are simple fabricate avoid porosity-related weakening. Here, we report, auxeticity for a non-porous liquid crystal elastomer. For strains above ~0.8 the director, elastomer becomes with maximum ratio measured date being -0.74 ± 0.03 larger most...
Summary Factor XIII is responsible for the cross-linking of fibrin γ-chains in early stages clot formation, whilst α-chain occurs at a slower rate. Although γ- and was previously shown to contribute stiffness, role both chains determining structure currently unknown. Therefore, aim this study determine individual α- γ-chain during its effects on structure. We made use recombinant fibrinogen (γQ398N/Q399N/K406R), which does not allow y-chain cross-linking. In absence cross-linking, intact D-D...
The properties of (1,3)-β-glucans (i.e., callose) remain largely unknown despite their importance in plant development and defence. Here we use mixtures (1,3)-β-glucan cellulose, ionic liquid solution hydrogels, as proxies to understand the physico-mechanical callose. We show that after callose addition stiffness cellulose hydrogels is reduced at a greater extent than predicted from ideal mixing rule weighted average individual components' properties). In contrast, yield behaviour elastic...
Biocompatible microgels have been demonstrated to act as excellent lubricants, however, the influence of continuum on their overall mechanical performance has neglected so far. In this work, colloidal whey protein (hydrodynamic diameter ∼100 nm measured using dynamic light scattering and atomic force microscopy) different rigidity dispersed in Newtonian (buffer corn syrup) or complex non-Newtonian fluids (xanthan gum) is investigated for first time via rheology soft tribology. Dispersions...
This study investigated the microstructure and interactions of heat treated concentrated Fusarium venenatum biomass, commonly referred to as 'mycoprotein' (MYC), well impact 3 wt % egg white protein (EWP) on those – EWP being frequently added a 'binding agent'. Confocal laser scanning microscopy (CLSM) cryo-scanning electron (Cryo-SEM) combined with energy dispersive spectroscopy analysis (EDS) demonstrated filamentous nature MYC, made up chitin protein, filament aspect ratio typically...
Hydroxyapatite crystals from developing rat incisor enamel and polished sections of mature human dental were investigated using Atomic Chemical Force Microscopy. Regular substructures seen on comprising ∼40 nm wide morphologically chemically defined bands across the crystal long axes. Exposure to low pH resulted in selective dissolution between emergence 2–3 spherical structures within each band. The distinct exhibiting high friction lateral force mode. Enamel appear comprise stacks roughly...
In this paper, we demonstrate that it is possible to observe many aspects of critical phenomena in supported lipid bilayers using atomic force microscopy (AFM) with the aid stable and precise temperature control. The regions criticality were determined by accurately measuring calculating phase diagrams for 2 L(d)-L(o) region, tracking how moves temperature, then increasing sampling density around estimated regions. Compositional fluctuations observed above (T(c)) characterised a spatial...
Through a multi-scale approach, the internal mechanics of microgel particles are probed, characterising radial differences in mechanical response.
Fluorescent probes are useful in biophysics research to assess the spatial distribution, mobility, and interactions of biomolecules. However, fluorophores can undergo "self-quenching" their fluorescence intensity at high concentrations. A greater understanding concentration-quenching effects is important for avoiding artifacts images relevant energy transfer processes photosynthesis. Here, we show that an electrophoresis technique be used control migration charged associated with supported...
Divalent and tetravalent analogues of ganglioside GM1 are potent inhibitors cholera toxin Escherichia coli heat-labile toxin. However, they show little increase in inherent affinity when compared to the corresponding monovalent carbohydrate ligand. Analytical ultracentrifugation dynamic light scattering have been used demonstrate that multivalent induce protein aggregation formation space-filling networks. This process appears arise using ligands do not match valency receptor. While it is...