A5052580392- Biochemical Analysis and Sensing Techniques
- Olfactory and Sensory Function Studies
- Advanced Chemical Sensor Technologies
- Regulation of Appetite and Obesity
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Helicobacter pylori-related gastroenterology studies
- Diet and metabolism studies
- Gastroesophageal reflux and treatments
- Eating Disorders and Behaviors
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Cholesterol and Lipid Metabolism
- Urinary Tract Infections Management
- Bacterial Identification and Susceptibility Testing
- Fecal contamination and water quality
- Diet, Metabolism, and Disease
- Probiotics and Fermented Foods
- Cancer Cells and Metastasis
- Dietary Effects on Health
KU Leuven
2019-2023
University of Vienna
2009-2021
Christian Doppler Laboratory for Thermoelectricity
2017-2018
Significance This study shows that caffeine's effect on gastric acid secretion (GAS) is more complex than has been previously thought. Oral and bitter taste receptors are involved in the regulation of GAS humans. regulatory process can be modified by bitter-masking compound homoeriodictyol. Practical applications results may include treatment gastroesophageal reflux disease or peptic ulcer manipulating pH means tastants inhibitors.
The gastrointestinal microbiota produces short-chain fatty acids, especially butyrate, which affect colonic health, immune function and epigenetic regulation. To assess the effects of nutrition aging on production butyryl-CoA:acetate CoA-transferase gene population shifts Clostridium clusters lV XlVa, main butyrate producers, were analysed. Faecal samples young healthy omnivores (24 ± 2.5 years), vegetarians (26 5 years) elderly (86 8 evaluated. Diet lifestyle assessed in questionnaire-based...
Bitter taste receptors (taste 2 receptors, TAS2Rs) serve as warning sensors in the lingual system against ingestion of potentially poisonous food. Here, we investigated functional role TAS2Rs human gut and focused on their potential to trigger an additional host defense pathway intestine. Human jejunal crypts, especially those from individuals with obesity, responded bitter agonists by inducing release antimicrobial peptides (α-defensin 5 regenerating islet–derived protein 3 α [REG3A]) but...
<i>Background/Aims:</i> This study aimed to investigate the quantitative and qualitative changes of bacteria, <i>Bacteroides</i>, <i>Bifidobacterium</i> <i>Clostridium</i> cluster IV in faecal microbiota associated with a vegetarian diet. <i>Methods:</i> Bacterial abundances were measured samples 15 vegetarians 14 omnivores using PCR. Diversity was assessed PCR-DGGE fingerprinting, principal component analysis (PCA) Shannon...
Chemosensory signaling in organs such as the mouth and gut contributes to mechanisms that control metabolism. We investigated chemosensory pathways regulate secretion of hunger hormone ghrelin response neurotransmitters, bitter sweet tastants at cellular level human mucosa, disturbances this regulatory pathway induced by obesity. Obesity impaired protein production adrenalin-induced fundic cells, which was counterbalanced somatostatin. Bitter agonists selective for taste receptor type 2...
Red wine is rich in phenolic compounds, which chiefly determine its characteristic taste. One of major acid constituents for an astringency, yet no clear contribution to bitter taste has been reported, gallic (GA). In previous studies, we have demonstrated bitter-tasting regulate cellular proton secretion (PS) as a key mechanism gastric via activation sensing receptors (TAS2Rs). Here, hypothesized contributing role GA the red wine-stimulated effect on PS human tumor cells (HGT-1 cells)....
Beer, one of the most consumed beverages worldwide, has been shown to stimulate gastric acid secretion. Although organic acids, formed by fermentation glucose, are known be stimulants secretion, very little is about effects different types beer or active constituents thereof. In present study, we compared beers on mechanisms To investigate compound-specific acids and bitter compounds were quantified HPLC-DAD UPLC-MS/MS tested in human cancer cells (HGT-1) means a pH-sensitive fluorescent dye...
Organic acids of wine, in addition to ethanol, have been identified as stimulants gastric acid secretion. This study characterized the influence other wine compounds, particularly phenolic on proton Forty parameters were determined four red wines and six white wines, including contents organic compounds. The secretory activity was a cell culture model (HGT-1 cells) by means pH-sensitive fluorescent dye. Red stimulated secretion more than wines. Lactic compounds syringic acid, catechin,...
Wine may cause stomach irritation due to its stimulatory effect on gastric acid secretion, although the mechanisms by which wine or components thereof activate pathways of secretion are poorly understood. Gastric pH was measured with a noninvasive intragastric probe, demonstrating that administration 125 mL white red healthy volunteers stimulated more potently than equivalent amounts ethanol. Between both beverages, showed clear trend for being active in stimulating (p = 0.054)....
Secretion of gastric acid, aimed at preventing bacterial growth and aiding the digestion foods in stomach, is chiefly stimulated by dietary intake protein amino acids (AAs). However, AAs' key structural determinants responsible for their effects on mechanisms regulating acid secretion (GAS) have not been identified yet. In this study, AAs tested parietal cell model HGT-1 GAS mRNA expression genes GAS. taste intensities from 0 (not bitter all) to 10 (very bitter) were assessed a sensory which...
Humans perceive bitterness via around 25 different bitter receptors. Therefore, the identification of antagonists remains a complex challenge. We previously demonstrated several bitter-tasting compounds such as caffeine to induce acid secretion in stomach and human gastric tumor cell line (HGT-1). Here, results fluorescent-based vitro assay using HGT-1 cells sensory panel testing nine selected potential modulators, with or without theobromine, were compared. Of bitter-modulating tested,...
Flavanoids and related polyphenols, among them hesperitin, have been shown to modulate cellular glucose transport by targeting SGLT-1 GLUT-2 proteins. We aimed investigate whether homoeriodictyol, which is structurally affects uptake in differentiated Caco-2 cells as a model for the intestinal barrier. The results revealed that, contrast other flavanon homoeriodictyol promotes 29.0 ± 3.83% at concentration of 100 μM. stimulating effect was sensitive phloridzin, but not phloretin, indicating...
Scope Anorexia of aging, characterized by a decrease in appetite and/or food intake, is major risk factor under‐nutrition and adverse health outcomes elderly people. Recent vitro evidence suggests homoeriodictyol (HED), naturally occurring, bitter‐masking flavanone, as promising agent to increase intake. Methods results In two cross‐over intervention trials, 30 mg NaHED, either solely ( n = 10, Study I) or combination with 75 g glucose load 17, study II) were administered healthy adult...
The noncaloric sweeteners (NCSs) cyclamate (Cycl) and acesulfame K (AceK) are widely added to foods beverages. Little is known about their impact on gastric acid secretion (GAS), which stimulated by dietary protein bitter-tasting compounds. Since Cycl AceK have a bitter off taste in addition sweet taste, we hypothesized they modulate mechanisms of GAS human parietal cells (HGT-1). HGT-1 were exposed tastants (50 mM glucose, d-threonine, Cycl, or AceK) analyzed for intracellular pH index...