A5103019715- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Computational Drug Discovery Methods
- Lipoproteins and Cardiovascular Health
- Galectins and Cancer Biology
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Complementary and Alternative Medicine Studies
- Algal biology and biofuel production
- EEG and Brain-Computer Interfaces
- Monoclonal and Polyclonal Antibodies Research
- Lipid metabolism and disorders
- Carbon Nanotubes in Composites
- MicroRNA in disease regulation
- Plant biochemistry and biosynthesis
- Hepatitis C virus research
- Veterinary medicine and infectious diseases
- Liquid Crystal Research Advancements
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Mindfulness and Compassion Interventions
Chinese Academy of Sciences
2014-2024
Shanghai Institute of Materia Medica
2020-2024
China Pharmaceutical University
2007-2024
State Key Laboratory of Natural Medicine
2016-2024
Capital Medical University
2019-2024
Binghamton University
2023-2024
National Clinical Research Center for Digestive Diseases
2023-2024
Soochow University
2024
Beijing Jiaotong University
2023
Institute of Electrical and Electronics Engineers
2023
The pandemic of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has become a global crisis. Replication SARS-CoV-2 requires the viral RNA-dependent RNA polymerase (RdRp) enzyme, target antiviral drug remdesivir. Here we report cryo-electron microscopy structure RdRp, both in apo form at 2.8-angstrom resolution and complex with 50-base template-primer remdesivir 2.5-angstrom resolution. reveals that partial double-stranded template is inserted...
Abstract The ongoing pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) urgently needs an effective cure. 3CL protease (3CL pro ) is a highly conserved cysteine proteinase that indispensable for replication, providing attractive target developing broad-spectrum antiviral drugs. Here we describe the discovery myricetin, flavonoid found in many food sources, as non-peptidomimetic and covalent inhibitor SARS-CoV-2 . Crystal structures...
The persistent pandemic of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2) and its variants accentuates the great demand for developing effective therapeutic agents. Here, we report development an orally bioavailable SARS-CoV-2 3C-like protease (3CL
Abstract Deep brain regions such as hippocampus, insula, and amygdala are involved in neuropsychiatric disorders, including chronic insomnia depression. Our recent reports showed that transcranial alternating current stimulation (tACS) with a of 15 mA frequency 77.5 Hz, delivered through montage the forehead both mastoids was safe effective intervening depression over 8 weeks. However, there is no physical evidence to support whether large tACS can send electrical currents deep tissue awake...
Abstract Human infections with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cause disease 19 (COVID-19) and there is currently no cure. The 3C-like protease (3CLpro), a highly conserved indispensable for replication of coronaviruses, promising target development broad-spectrum antiviral drugs. To advance the speed drug discovery development, we investigated inhibition SARS-CoV-2 3CLpro by natural products derived from Chinese traditional medicines. Baicalin baicalein were...
Cyclic GMP–AMP synthase (cGAS) has been recently uncovered to be a promising therapeutic target for immune-associated diseases. Until now, only few inhibitors have identified through high-throughput screening campaigns. Here, we reported the discovery of novel catalytic domain human cGAS (h-cGASCD) by virtual first time. To generate reliable docking mode, obtained high-resolution crystal structure h-cGASCD in complex with PF-06928215, known inhibitor h-cGAS, followed molecular dynamics...
Chinese Cantonese opera, a UNESCO Intangible Cultural Heritage (ICH) of Humanity, has faced series development problems due to diversified entertainment and emerging cultures. While, the management on opera data in scientific manner is conducive sustainable ICH. Therefore, this study, standardized audio database dedicated established, classification method for singing genres based Genre Classification Networks (CoGCNet) model proposed given similarity rhythm characteristics different genres....
The pandemic of Corona Virus Disease 2019 (COVID-19) caused by SARS-CoV-2 has become a global crisis. replication requires the viral RNA-dependent RNA polymerase (RdRp), direct target antiviral drug, Remdesivir. Here we report structure RdRp either in apo form or complex with 50-base template-primer and Remdesivir at resolution range 2.5-2.8 Å. reveals that partial double-stranded template is inserted into central channel where incorporated first replicated base pair terminates chain...
Oxidative stress-induced vascular endothelial cell (VEC) injury is a major mechanism in the initiation and development of atherosclerosis. Lunasin, soybean-derived 43-aa peptide, has been previously shown to possess potent antioxidant anti-inflammatory activities other than its established anticancer activities. This study investigated effects lunasin on protecting VECs from oxidative damage inhibiting atherosclerotic plaque progression apolipoprotein E-deficient (ApoE
Ca(2+) is the main element of many pulp capping materials that are used to promote regeneration tertiary dentin, but underlying molecular mechanism not clear. In this study, we found increased expression odontoblastic differentiation marker gene DSPP and promoted mineralization DPSCs, inhibited ALP activity. increases endogenous BMP2, which activates Smad1/5/8 pathway promotes Smad1-Runx2 Runx2-DSPP interaction in DPSCs. Inhibition with dorsomorphin partially blocked Runx2 activity; however,...
Abstract Background As the resistance of Helicobacter pylori to traditional triple therapy is gradually revealed, an increasing number people are focusing on vaccine treatments for H . infection. Epitope vaccines a promising strategy treatment infection, and multivalent will be more effective than monovalent vaccines. Materials methods In this study, we designed named LHUC, which consists adjuvant LTB as well three Th cell epitopes (HpaA 154‐171 , UreB 237‐251, 546‐561 ) five B‐cell (UreB...
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a serine protease which regulates serum low-density lipoprotein cholesterol (LDL-C) levels by promoting the degradation of hepatic receptor (LDLR), and has become an attractive therapeutic target for lowering intervention. Lunasin, 43-amino acid polypeptide initially isolated from soybean, been previously proven to possess activity. Here we identified down-regulation PCSK9 expression lunasin as one new mechanism that increased...
The coronavirus papain-like protease (PLpro ) plays an important role in the proteolytic processing of viral polyproteins and dysregulation host immune response, providing a promising therapeutic target. However, development inhibitors against severe acute respiratory syndrome 2 (SARS-CoV-2) PLpro is challenging owing to restricted S1/S2 sites substrate binding pocket. Here we report discovery two activators SARS-CoV-2 identification unique residue, cysteine 270 (C270), as allosteric...
This paper presents a focused investigation into real-time segmentation in unstructured environments, crucial aspect for enabling autonomous navigation off-road robots. To address this challenge, an improved variant of the DDRNet23-slim model is proposed, which includes lightweight network architecture and reclassifies ten different categories, including drivable roads, trees, high vegetation, obstacles, buildings, based on RUGD dataset. The model's design integration semantic-aware...
Study Design. To evaluate the effect of p38 pathway on spinal cord injury (SCI), a rat model SCI was performed. Objective. We determined and related inflammation, apoptosis, autophagy. Summary Background Data. is severe clinical problem worldwide. It difficult to prevent cell necroptosis promote survival residual neurons after SCI. p38, class mitogen-activated protein kinases, its autophagy have not been studied very well. Methods. The rats were randomly divided into following four groups:...
USP7 has been regarded as a potential therapeutic target for cancer. In this study, virtual screening, molecular dynamics (MD) simulation, and biological evaluation have applied the discovery of novel inhibitors targeting catalytic active site. Among obtained compounds, compound 12 with scaffold structure exhibited certain inhibitory activity (Ub-AMC assay IC50 = 18.40 ± 1.75 μM, Ub-Rho 7.75 μM). The binding affinity between USP7CD (USP7 domain) hit was confirmed KD value 4.46 0.86 μM....