Background & Objective: Pediatric acute leukemias can present with aberrant immunophenotypes characterized by a different pattern of antigen expression on malignant cells, unlike the process usual hematopoietic maturation. The objective this study was to determine immunophenotype expressions in newly diagnosed pediatric lymphoblastic and myeloid leukemias. Methods: This cross-sectional carried out at University Child Health Sciences, Children’s Hospital, Lahore, from October 2022...

Abstract: BACKGROUND: Blood transfusion therapy is the main for thalassemia patients. Active reporting of acute reactions during blood transfusions can play a pivotal role in prevention under-reporting these events. OBJECTIVE: The objective study was to evaluate true incidence pediatric patients using active surveillance comparison with data obtained from passive same duration time. MATERIALS AND METHODS: This cross-sectional cohort comprised both prospective (active surveillance) and...

Objectives: To analyze whether leucopenia and lymphopenia a characteristic feature of children with COVID-19 to find out its association the disease severity.
 Methods: This was descriptive cross-sectional study conducted at The Children’s Hospital Lahore from March 2020 October 2020. All confirmed cases infection post-COVID MIS-C/Kawasaki Disease diagnosed on basis RT-PCR Antibody test respectively were included. Complete blood differential counts performed day admission.
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Objective: To compare the platelet count, concentration/yield, residual Red blood cells (RBCs) and White (WBCs) counts in platelet-rich plasma (PRP) samples prepared from single- double-centrifugation protocols.
 Methods: It was a Cross-Sectional study, conducted at Department of Hematology & Transfusion Medicine, The Children’s Hospital UCHS, Lahore October 2021 to January 2022 including 50 voluntary, healthy individuals age 20-45 years both genders, after taking informed consent....

Congenital afibrinogenemia (OMIM #202400) is a rare coagulation disorder that was first described in 1920. It transmitted as an autosomal recessive trait characterized by absent levels of fibrinogen (factor I) plasma. Consanguinity Pakistan and its neighboring countries has resulted higher number cases congenital deficiency their respective populations. This study focused on the detection mutations genes using DNA sequencing molecular modeling missense all three [Fibrinogen gene alpha (FGA),...

This study was designed to gross morphological and basic osteometric features of the digit bones Chinkara.The osteometry were performed manually through a digital vernier calliper on twenty (20) adult Chinkara (10 each male females).The digits in limb comprised lateral medial proximal, middle distal phalanx.The first two phalanges long bones.The shaft phalanx proximalis (P1) thick proximally than distally slightly arched.The media (P2) shorter length proximal.Its proximal articular surface...

The participation of women in blood donation process has been scarce equallyin low & high resourced countries. Every year 3.5 million donations are collected inPakistan with very little percentage females donating blood. Objective: objective thestudy was to assess the knowledge, attitude and beliefs female attendants accompanyingthe patients. Study Design: Cross-sectional study. Setting: Children’s Hospital Instituteof Child Health, Lahore, regarding donation. Period: 1st June 31st...

Type 3 von Willebrand disease (VWD), a severe autosomal recessive hereditary bleeding disorder, is described by the virtual absence of factor (VWF). In consanguineous populations, for example Pakistan, reported with higher incidence rate than worldwide prevalence.This study aims to characterize molecular pathology and clinical profile type VWD cohort Pakistani origin.In total, 48 patients were enrolled in current study. Initially, index (IPs) evaluated standardized questionnaire recording...

Objectives: To assess the utility of ISTH-BAT (International Society on Thrombosis and Hemostasis- Bleeding Assessment Tool) in diagnosis Glanzmann Thrombasthenia (GT) comparison to controls.
 Methods: It was a case-control study carried out at The Children’s Hospital, Lahore from January 2012 May 2021. All patients neonates 18 years with final GT were studied retrospectively. clinical details collected hospital records telephonically questionnaire after taking informed consent. same...

To determine the effect of educational interventions on knowledge and approach Allied Health Sciences Students regarding blood groups donation.

Background: In 1939 Rh antigen was discovered by Levine and Stetson. Rhsystem antigens are very immunogenic, they can produce significant Hemolytic Disease of thefetus Newborn as well hemolytic transfusion reactions. There numerous variants ofD, the most common subtypes Weak D Partial D, now called abnormal antigens.The incidence negativity worldwide varies between 3%-25% that weak antigenranges from 0.2%-1%. Objectives: To find out frequency Dantigen among donors coming to blood bank The...

Background: COVID-19 has presented with varied signs and symptoms raised inflammatory markers among which D-dimers have been highlighted right from the beginning. Objectives were to analyze relationship of disease severity outcome children MIS-C in a developing country. Methods: It was retrospective study conducted at pediatric tertiary care hospital March 2020 November 2021. Clinical laboratory details confirmed post cases recorded. D-dimer testing performed using Chemiluminescence...

Background: To find the association of blood donation related fears with donors’ characteristics like age, education level and previous history to their impact on future donation. Methods: It was a cross-sectional study carried out at tertiary care hospital in Lahore, Pakistan, from June December 2022. A self-designed questionnaire addressing five types filled 700 donors through interview after taking informed consent. Data analyzed by using IBM-SPSS V23. Results: Among participants, 91.1%...

Objective: To determine the frequency of ABO discrepancies in pediatric patients lymphoma and solid organ tumors to categorize these their resolution. Study Design: Cross-sectional study. Setting: Department Hematology & Transfusion Medicine, The University Child Health Sciences Children’s Hospital, Lahore. Period: November 2020 September 2021. Material Methods: blood group were assessed by tube method grouping, using antisera A, B, AB D for forward grouping O cells reverse grouping....

The participation of women in blood donation process has been scarce equallyin low & high resourced countries. Every year 3.5 million donations are collected inPakistan with very little percentage females donating blood. Objective: objective thestudy was to assess the knowledge, attitude and beliefs female attendants accompanyingthe patients. Study Design: Cross-sectional study. Setting: Children’s Hospital Instituteof Child Health, Lahore, regarding donation. Period: 1st June 31st...

Objective: To Estimate the frequency of blood group discrepancies among Leukemia Children. Study Design: Cross Sectional study. Setting: University Child Health Sciences, The Children Hospital Lahore. Period: October to January 2020. Material & Methods: This study was conducted patients in CH and ICH Lahore, regarding ABO leukemic patients, year 2019. A total 200 samples were processed order detect by tube method grouping, using antisera-A, antisera-B, antisera-D for forward grouping...

Aim: To determine frequency of alloimmunization and autoimmunization among the patient thalassemia with history multiple blood transfusions. Methods: This study was conducted by consecutive sampling in six months duration from September 2020 to August 2021 at department Hematology Transfusion Medicine, University Child Health Sciences, The Children's Hospital Lahore. Any a positive Direct antiglobulin test (DAT) labeled as auto-immunized indirect (IAT) alloimmunized. Results: Total 90 cases...

Lymphoblastic lymphoma and chronic myeloid leukaemia (CML) are two distinct neoplasms with different pathogenesis clinical presentation.We hereby share a challenging case of child presenting fever, leucocytosis, generalised lymphadenopathy massive splenomegaly.He was diagnosed as having novel association concurrent T-lymphoblastic on cervical lymph node biopsy BCR-ABL negative CML bone marrow aspirate.The study more such cases is needed for optimal patient management.

Background: In 1939 Rh antigen was discovered by Levine and Stetson. Rhsystem antigens are very immunogenic, they can produce significant Hemolytic Disease of thefetus Newborn as well hemolytic transfusion reactions. There numerous variants ofD, the most common subtypes Weak D Partial D, now called abnormal antigens.The incidence negativity worldwide varies between 3%-25% that weak antigenranges from 0.2%-1%. Objectives: To find out frequency Dantigen among donors coming to blood bank The...