A5101841777- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Musculoskeletal pain and rehabilitation
- Reproductive Biology and Fertility
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Animal Disease Management and Epidemiology
- Reproductive biology and impacts on aquatic species
- Mitochondrial Function and Pathology
- Animal Genetics and Reproduction
- Viral Infections and Vectors
- DNA Repair Mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Pancreatic function and diabetes
- Pain Mechanisms and Treatments
- Blood groups and transfusion
- Viral gastroenteritis research and epidemiology
- Hemoglobinopathies and Related Disorders
- Turtle Biology and Conservation
- Animal Virus Infections Studies
- Peripheral Nerve Disorders
- Neurological diseases and metabolism
- Ear and Head Tumors
Aditya Birla (India)
2023-2024
Lasbela University of Agriculture Water and Marine Science
2016-2024
Balochistan University of Information Technology, Engineering and Management Sciences
2017-2024
Combined Military Hospital
2024
Armed Forces Institute of Pathology
2023
Government College University, Lahore
2022
University of Science and Technology of China
2022
Dalian Medical University
2021
Nanjing Agricultural University
2015-2020
Mayo Hospital
2015-2019
Apoptosis is a tightly controlled process by which tissues eliminate unwanted cells. Spontaneous germ cell apoptosis in testis has been broadly investigated mammals that have an associated spermatogenesis pattern. However, the mechanism of seasonally breeding reptiles following dissociated remained enigmatic. In present study, morphological evidence clearly confirmed pattern Pelodiscus sinensis. TUNEL and TEM analyses presented dynamic changes ultrastructural characteristics apoptotic cells...
Sertoli cells (SCs) play a central role in the development of germ within functional testes and exhibit varying morphology during spermatogenesis. This present study investigated seasonal morphological changes SCs reproductive cycle Pelodiscus sinensis by light microscopy, transmission electron microscopy (TEM), immunohistochemistry. During hibernation period with quiescent spermatogenesis, several autophagosomes were observed inside SCs, processes which retracted. In early when started to...
Abstract Important evolutionary and ecological consequences arise from the ability of female turtles to store viable spermatozoa for an extended period. Although previous morphological studies have observed localization in Pelodiscus sinensis oviduct, no systematic study on identification genes that are involved long-term sperm storage has been performed. In this study, oviduct P. at different phases (reproductive hibernation seasons) was prepared RNA-Seq gene expression profiling. total,...
Asthenoteratozoospermia is one of the most severe types qualitative sperm defects. Most cases are due to mutations in genes encoding components flagella, which have an ultrastructure similar that motile cilia. Coiled-coil domain containing 103 ( CCDC103 ) outer dynein arm assembly factor, and pathogenic variants cause primary ciliary dyskinesia (PCD). However, whether asthenoteratozoospermia has yet be determined. Whole-exome sequencing (WES) was performed for two individuals with...
Autophagy is a unique catabolic pathway that linked to several physiological processes. However, its role in the process of spermiogenesis largely unknown. The aim current study was determine vivo autophagy and origin autophagosome membrane biogenesis within male haploid cells. Our immunohistochemistry results demonstrated LC3 ATG7 localization were increased dramatically round elongated spermatids (haploid cells) towards lumen seminiferous tubules, however, poorly expressed early stages...
Congenital afibrinogenemia (OMIM #202400) is a rare coagulation disorder that was first described in 1920. It transmitted as an autosomal recessive trait characterized by absent levels of fibrinogen (factor I) plasma. Consanguinity Pakistan and its neighboring countries has resulted higher number cases congenital deficiency their respective populations. This study focused on the detection mutations genes using DNA sequencing molecular modeling missense all three [Fibrinogen gene alpha (FGA),...
Telocytes are a novel type of interstitial cell that has been identified in many organs mammals, but there is little information available on these cells avian species. This study shows the latest findings associated with telocytes muscular layer and lamina propria magnum chicken oviduct analyzed by transmission electron microscopy. characterized telopodes, which thin long prolongations, small amount cytoplasm rich mitochondria. Spindle- or triangular-shaped were detected at various...
Aging | doi:10.18632/aging.101070. Nisar Ahmed, Yi Liu, Hong Chen, Ping Yang, Yasir Waqas, Tengfei Jameel Ahmed Gandahi, Yufei Huang, Lingling Wang, Xuejing Song, Imran Rashid Rajput, Taozhi Qiusheng Chen
Duck Tembusu virus (DTMUV) is a novel member of Flavivirus. The isolated and purified DTMUV strain XZ-2012 was used as model, to intramuscularly inject the six-month egg-laying shelducks with infective dose 104TCID50. dynamic distribution in spleen at different time post-infection (pi) studied using RT-PCR, RT-qPCR, ELISA, immunofluorescence transmission electron microscopy (TEM).The results showed that occurred after 2 hpi lasted up 18 dpi. registered viral load increased from 3 dpi, then...
Multiple morphological abnormalities of the flagella (MMAF) represent a severe form sperm defects leading to asthenozoospermia and male infertility. In this study, we identified novel homozygous splicing mutation (c.871-4 ACA>A) in adenylate kinase 7 ( AK7 ) gene by whole-exome sequencing infertile individuals. Spermatozoa from affected individuals exhibited typical MMAF characteristics, including coiled, bent, short, absent, irregular flagella. Transmission electron microscopy analysis...
The present study was designed to investigate the hypothesis that in vivo entosis is a novel pathway for eliminating spermatozoa seminiferous tubules (ST) during hibernation of Chinese soft-shelled turtle. Western blot analysis revealed expression LAMP1 testis significantly higher than non-hibernation. Immunohistochemistry reaction showed LAMP1-positive substance distributed within Sertoli cells testis. Further examination by transmission electron microscopy (TEM), many degraded being...
Genetic variations (mutation, crossing over, and recombination) act as a source for the gradual alternation in phenotype along geographic transect where environment changes. Posttranslational modifications (PTMs) predicted successfully different same species of living organisms. Protein diversity organisms is by PTMs. Environmental stresses change nucleotides to produce alternations protein structures, these have been examined through bioinformatics tools. The goal current study search genes...
Lumbar disc herniation (LDH) with radiculopathy is one of the most common diagnoses encountered in orthopedic clinical practice and it believed to be a major contributor estimated 60-80% lifetime incidence low back pain. This study was designed compare effects two dissimilar manual therapy techniques viz neural tissue mobilization Mulligan’s spinal limb movement (SMWLM) patients L4/L5-L5/S1 lumbar associated radiculopathy. A pre-test post-test experimental design using random sampling used...
Fanconi anemia is a rare autosomal recessive disorder of genetic instability. It both molecularly and clinically, heterogeneous disorder. Its incidence 1 in 129,000 births relatively high some ethnic groups. Sixteen genes have been identified among them mutations FANCG gene are most common after FANCA FANCC mutations.To study exon 3 4 Pakistani population.Thirty five patients with positive Diepoxybutane test were included the study. DNA was extracted amplified for exons 4. Thereafter...
We report opacification of 2 multifocal intraocular lenses (IOLs). Patients with these IOLs may be more likely to require IOL explantation than those monofocal IOLs.
The study was done to find out whether nerve mobilization techniques enhance patient outcomes in the management of sciatica when added standard care. A total 30 patients, both male and female with a primary diagnosis were recruited from outpatient physical therapy department G.B. Pant Neurology Hospital, New Delhi. Pre-test, post-test experimental design used. After measuring baseline pain (NPRS) functional status (SF-12) scores, one group treated Nerve Mobilization along conventional while...
This study aimed to (1) identify F9 genetic alterations in patients with hemophilia B (HB) of Pakistani origin and (2) determine the genotype-phenotype relationships these patients. Diagnosed cases HB were identified through registries at designated tertiary health-care centers across country. Consenting enrolled into study. The factor IX (FIX) coagulation activity (FIX:C) key clinical features recorded. Direct sequencing was carried out all All variants analyzed for functional consequences...
In poultry, the infundibulum is place of fertilization, eggshell production, and sperm storage, while its uterovaginal junction (UVJ) regarded as most important site, which has abundant storage tubules (SST). We examined ultrastructure epithelium with relation to unique secretory cytology in UVJ hens using transmission electron microscopy. The lined ciliated cells. Ciliated cells are characterized light dense granules supernuclear cytoplasm. Dense larger diameter (one μm), surrounded a...
The dermal microvascular unit (DMU) is a perivascular functional in the dermis. It composed of and capillary lymphatics surrounded by immune cells. In this study, jet needle-free injection system was used to injected biocompatible carbon nanoparticles into cervical skin domestic pigs (Sus scrofa domestica) assessed morphological distribution DMUs hematoxylin erythrosine staining, immunohistochemistry (IHC), transmission electron microscopy (TEM), TEM also observe ultrastructural changes...