A5100726338- Fungal Biology and Applications
- Nanoplatforms for cancer theranostics
- Food Quality and Safety Studies
- Pharmacological Effects of Natural Compounds
- Plant and fungal interactions
- Chemical synthesis and alkaloids
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Additive Manufacturing and 3D Printing Technologies
- Alkaloids: synthesis and pharmacology
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Advanced Nanomaterials in Catalysis
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Aldose Reductase and Taurine
- Manufacturing Process and Optimization
- Plant tissue culture and regeneration
- Glioma Diagnosis and Treatment
- Circular RNAs in diseases
- Sperm and Testicular Function
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Nuclear Receptors and Signaling
- Cancer Research and Treatments
The First Affiliated Hospital, Sun Yat-sen University
2016-2025
Zhongshan Hospital
2016-2025
Fudan University
2013-2025
Sun Yat-sen University
2016-2025
Shengli Oilfield Central Hospital
2025
State Key Laboratory of Digital Medical Engineering
2022-2024
Yunnan University
2021-2024
Nanfang Hospital
2017-2024
Southern Medical University
2007-2024
Army Medical University
2017-2024
Abstract Epstein–Barr virus (EBV), aetiologically linked to nasopharyngeal carcinoma (NPC), is the first human found encode many miRNAs. However, how these viral miRNAs precisely regulate tumour metastasis in NPC remains obscure. Here we report that EBV-miR-BART1 highly expressed and closely associated with pathological advanced clinical stages of NPC. Alteration expression results an increase migration invasion cells vitro causes vivo . Mechanistically, directly targets cellular suppressor...
Abstract Background Accumulating evidence indicates that circular RNAs (circRNAs) act as microRNA (miRNA) sponges to directly inhibit specific miRNAs and alter their ability regulate gene expression at the post-transcriptional level; this mechanism is believed occur in various cancers. However, level, precise function of circ_001680 colorectal carcinoma (CRC) are largely unknown. Methods qRT-PCR was used detect miR-340 human CRC tissues matched normal tissues. Bioinformatics analyses...
Imaging-guided real-time monitoring of the treatment process inflammatory diseases is important for timely adjustment planning to lower unnecessary side effects and improve outcomes. However, it difficult reflect dynamic changes inflammation in vivo with enough tissue penetration depth. Here a novel nanotheranostic agent (denominated TMSN@PM) platelet membrane (PM)-coated, tempol-grafted, manganese-doped, mesoporous silica nanoparticles developed. The PM endows TMSN@PM ability target sites,...
Author: Please verify that the changes made to improve English still retain your original meaning.Detection of microRNA (miRNA) in dermal interstitial fluid (ISF) has emerged as clinically useful health status monitoring. However, it remains a great challenge owing difficult sampling and low abundance. Here, we report DNA hydrogel microneedles (MNs) array realize rapid enrichment sensitive detection miRNA ISF. The MNs' patch consists methacrylate hyaluronic acid (MeHA) equipped with smart...
Background Identification of gene variants plays an important role in research on and diagnosis genetic diseases. A combination enrichment targeted genes next-generation sequencing (targeted DNA-HiSeq) results both high efficiency low cost for interest. Methodology/Principal Findings To identify mutations associated with diseases, we designed array-based chip to capture all the exons 193 involved 103 evaluate this technology, selected 7 samples from seven patients six different diseases...
AKR1C3 is an enzyme belonging to the aldo-ketoreductase family, members of which catalyze redox transformations involved in biosynthesis, intermediary metabolism, and detoxification. plays important role tumor progression metastasis, however, little known about function molecular mechanism underlying hepatocellular carcinoma (HCC). In this study, we report that significantly upregulated HCC increased associated with poor survival. positively regulated cell proliferation metastasis
Yeast cell factories have been increasingly employed for producing plant-derived natural products. Unfortunately, the stability of plant product biosynthetic pathway genes, particularly when driven by same sets promoters and terminators, remains one biggest concerns synthetic biology. Here we profile genomic loci flanked essential genes as stable integration sites in a genome-wide manner, maintenance multigene pathways yeast. We demonstrate application our yeast platform construction...
Abstract The highly widespread and infiltrative nature of glioblastoma multiforme (GBM) makes complete surgical resection hard, causing high recurrence rate poor patients’ prognosis. However, the mechanism underlying GBM migration invasion is still unclear. In this study, we investigated role a Ras-related protein Rab32 on uncovered its molecular subcellular mechanisms that contributed to aggressiveness. correlation expression with patient prognosis tumor grade was by public dataset analysis...
Epstein-Barr virus (EBV)-encoded molecules have been detected in the tumor tissues of several cancers, including nasopharyngeal carcinoma (NPC), suggesting that EBV plays an important role tumorigenesis. However, nature with respect to genome width vivo and whether undergoes clonal expansion are still poorly understood. In this study, next-generation sequencing (NGS) was used sequence DNA extracted directly from tissue a patient NPC. Apart human sequences, clinically isolated 164.7 kb size...
Phytophthora root and stem rot caused by the oomycete pathogen sojae is a destructive disease of soybean worldwide. Plant dirigent proteins (DIR) are proposed to have roles in biosynthesis either lignan or lignin-like molecules, important for defense responses, secondary metabolism, resistance. In present work, novel DIR gene expressed sequence tag (EST) identified as up-regulated highly resistant cultivar 'Suinong 10' inoculated with P. sojae. The full length cDNA isolated using rapid...
Leaf growth and senescence are controlled by tight genetic factors involved regulation at multiple levels. Circular RNAs (circRNAs) have recently been reported as the microRNA sponge to accomplish corresponding regulatory roles. This study aims explore expression profile functional role of circRNAs in Arabidopsis leaf senescence. We used publically available RNA-seq data leaves identify circular RNA quantitative real-time PCR validate our identified circRNAs. The functions were explored...
Abstract Background Bacterial infection is one of the most common complications in burn, trauma, and chronic refractory wounds an impediment to healing. The frequent occurrence antimicrobial-resistant bacteria due irrational application antibiotics increases treatment cost mortality. Graphene oxide (GO) has been generally reported possess high antimicrobial activity against a wide range vitro. In this study, graphene oxide-quaternary ammonium salt (GO-QAS) nanocomposite was synthesized...
Abstract Transdermal drug delivery is a crucial extension of administration routes and has been widely acknowledged as an alternative way to traditional oral subcutaneous injections due the advantages such increased dosage efficacy, decreased systemic side effect, improved patient compliance. The past few decades have witnessed biomedical applications microneedles in various cutting‐edge fields. Microneedles are needles with micron‐scale length which can pierce epidermis skin minimally...
Community detection is an important task with many applications. However, there no universal definition of communities, and a variety algorithms have been proposed based on different assumptions. In this paper, we instead study the semi-supervised community problem where are given several communities in network as training data aim to discover more communities. This setting makes it possible learn concepts from without any prior knowledge. We propose Seed Expansion generative Adversarial...
Abstract Background Agrobacterium -mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding plant biology. While some species, including soybean, still recalcitrant time-consuming, hampering the high-throughput analysis of soybean genes. Thus we pursue to develop rapid, simple, highly hairy root system induced by rhizogenes ( A. ) analyze function. Results In this report, was described. Only sixteen days were required whole...
Abstract Background High-order chromatin structure plays important roles in gene regulation. However, the diversity of three-dimensional (3D) genome across plant accessions are seldom reported. Results Here, we perform pan-3D analysis using Hi-C sequencing data from 27 soybean and comprehensively investigate relationships between 3D genomic variations structural (SVs) as well expression. We find that intersection regions A/B compartments largely contribute to compartment divergence....
B cell receptor associated protein 31 (BAP31) is closely with tumor progression, while the role and mechanism of BAP31 in gastric cancer (GC) remains unknown. This study explored that was upregulated GC tissues high expression indicated poor survival patients. knockdown inhibited growth induced G1/S arrest. Moreover, attenuation increased lipid peroxidation level membrane facilitated cellular ferroptosis. Mechanistically, regulated proliferation ferroptosis by directly binding to VDAC1...
Accumulating evidence shows that exosomes participate in cancer progression.However, the functions of cell exosome-transmitted proteins are rarely studied.Previously, we reported serglycin (SRGN) overexpression promotes invasion and metastasis esophageal squamous carcinoma (ESCC) cells.Here, investigated paracrine effects from SRGN-overexpressing ESCC cells (SRGN Exo) on tumor angiogenesis, used mass spectrometry to identify exosomal involved.Cation-dependent mannose-6-phosphate receptor...
We developed a new human papillomavirus (HPV) genotyping assay based on multiplex polymerase chain reaction and next-generation sequencing (NGS) methods for large-scale cervical cancer screening.We first trained the 1,170 self-collected samples, balancing cutoff points high-risk types. Then using 4,262 separate specimens, we compared concordance, sensitivity, specificity intraepithelial neoplasia type 2 (CIN2) or higher CIN 3 (CIN3) of HPV with that Hybrid Capture (HC2) direct samples...