A5057414183- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- Viral gastroenteritis research and epidemiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Protein purification and stability
- Neonatal and Maternal Infections
- Molecular Sensors and Ion Detection
- Seaweed-derived Bioactive Compounds
- Plant and Fungal Interactions Research
- Veterinary medicine and infectious diseases
- Gas Sensing Nanomaterials and Sensors
- Fungal Biology and Applications
- Virus-based gene therapy research
- Polysaccharides and Plant Cell Walls
- Skin Protection and Aging
- Male Reproductive Health Studies
Jiangsu Academy of Agricultural Sciences
2014-2025
Jiangsu Provincial Center for Disease Control and Prevention
2020
Background/Objectives: Targeted delivery of antigens to dendritic cells (DCs) is an effective strategy for enhancing vaccine efficacy. Methods: In this study, dual-targeting fusion proteins (GRFT-VHH54 and GRFT-VHH74) were constructed by fusing Griffithsin (GRFT), algae-derived lectin with enveloped virus-binding properties, DC-specific binding nanobodies (VHH54 VHH74). Vaccines formulated combining the inactivated H9N2 avian influenza virus these proteins, potential enhance vaccine-induced...
DNA delivery with bacteriophage by surface-displayed mammalian cell penetrating peptides has been reported. Although, various phages have used to facilitate transfer surface displaying the protein transduction domain of human immunodeficiency virus type 1 Tat (Tat peptide), no similar study conducted using T7 phage.In this study, we engineeredT7 phage as a targeting vector cellular internalization. We constructed recombinant that displayed peptide on their and carried eukaryotic expression...
Vaccination is the principal strategy for prevention and control of diseases, adjuvant use an effective to enhance vaccine efficacy. Traditional mineral oil-based adjuvants have been reported with post-immunization reactions. Developing new formulations improved potency safety will be great value.In study herein, a novel oil-in-water (O/W) Emulsion Adjuvant containing Squalane (termed EAS) was developed, characterized investigated swine influenza virus immunization. The data show that EAS...
Foot-and-mouth disease (FMD), a serious, fast-spreading, and virulent disease, has led to huge economic losses people all over the world. Vaccines are most effective way control FMD. However, weak immunogenicity of inactivated FMD virus (FMDV) requires addition adjuvants enhance immune effectiveness vaccines. Herein, we formulated fabricated biodegradable dendritic mesoporous tetrasulfide-doped organosilica nanoparticles SOMSN with imiquimod complex (SOMSN-IMQ) used it as platform for...
Adjuvants are essential for the induction of robust immune responses against vaccine antigens. Small-molecule TLR7 agonists hold high potential this purpose. In communication, imiquimod (IMQ) bearing a cholesterol lipid moiety derivative, IMQ-Chol, was designed and synthesized as adjuvant, which could release parent IMQ molecules in aqueous conditions via amide bond hydrolysis. We performed series immunological evaluations by cooperating with inactivated foot-and-mouth disease virus (FMDV)....
In this study, to improve the quality of a live attenuated vaccine for duck viral hepatitis (DHV), lyophilization heat-resistant virus was optimized. The optimized heat protectors were made 10% sucrose, 1.2% pullulan, 0.5% PVP, and 1% arginine, etc., with titer freeze-drying loss ≤0.50 Lg. product's valence measurements demonstrated following: could be stored at 2-8 °C 18 months ≤0.91 Lg; 37 10 days ≤0.89 45 3 ≤0.90 Regarding safety, no deaths occurred in two-day-old ducklings immunized...
Abstract Dendritic cells (DCs) are the antigen-presenting that initiate and direct adaptive immune responses, thus critically important in vaccine design. Although DC-targeting vaccines have attracted attention, relevant studies on chicken rare. A high diversity T7 phage display nanobody library was constructed for bio-panning of intact bone marrow DCs to find DC-specific binding nanobodies. After three rounds screening, 46 unique sequence clones were identified from 125 randomly selected...
Hydrogen sulfide (H2S) plays a vital role in regulating many important physiological functions. However, H2S-containing industrial wastewater is inevitably pumped into the environment which seriously contaminates water supplies and foodstuffs. So it crucial to develop single fluorescent probe for H2S detection with high sensitivity selectivity. Herein, colorimetric turn-on near infrared (NIR) NRDNP based on benzophenoxazine was designed synthesized by thiolysis of 2,4-dinitrophenyl (DNP)...
Bacteriophage T7 gene 17.5 coding for the only known holin is one of components its lysis system, but activity in more complex than a single gene, and evidence points to existence additional genes with activity. In this study, phage deletion (T7-△holin) was rescued biological characteristics effect on cell were determined. Furthermore, genomic evolution mutant T7-△holin during serial passage assessed by whole-genome sequencing analysis. It observed that from delays time enlarges burst size;...
The dermal microvascular unit (DMU) is a perivascular functional in the dermis. It composed of and capillary lymphatics surrounded by immune cells. In this study, jet needle-free injection system was used to injected biocompatible carbon nanoparticles into cervical skin domestic pigs (Sus scrofa domestica) assessed morphological distribution DMUs hematoxylin erythrosine staining, immunohistochemistry (IHC), transmission electron microscopy (TEM), TEM also observe ultrastructural changes...
The amine vapour produced by microorganisms is an important indicator of food spoilage. Amines are also ubiquitous in the chemical industry. development molecule-based ion sensors has been a pivotal issue that currently receiving considerable attention. In this work, new pyrylium salt-based fluorescent probe MTPY developed as rapid, highly sensitive, and selective sensor for methylamine vapour. exhibits obvious fluorescence response from yellow to cyan towards CH3NH2 calibration curve...
The aim of this study was to optimize the preparation conditions salidroside liposome with high encapsulation efficiency (EE) and immunological enhancement activity as porcine circovirus type 2 virus (PCV-2) vaccine adjuvant. Response surface methodology (RSM) selected for using Design-Expert V8.0.6 software. Three kinds adjuvants were prepared their adjuvant activity. BALB/c mice immunized PCV-2 encapsulated in different adjuvants. PCV-2-specific IgG serum determined ELISA. results showed...
Introduction There is a growing demand for effective technologies the delivery of antigen to antigen-presenting cells (APCs) and their immune-activation success DNA vaccines. Therefore, dendritic cell (DC)-targeting T7 phages were used as vehicle deliver vaccine. Methods In this study, eukaryotic expression plasmid pEGFP-C1-HA2-AS containing HA2 gene derived from avian H5N1 virus an anchor sequence (AS) required phage packaging process was developed. To verify feasibility delivery,...
A new amphiphilic polymer FIP provided a novel protein-based vaccine delivery platform, which was capable of inducing strong humoral and cellular immunity for effective prevention with acceptable safety.
Abstract Vacuum foam drying (VFD) has been shown to improve the thermostability and long-term shelf life of Newcastle Disease Virus (NDV). This study optimized VFD process NDV at laboratory-scale then tested conditions pilot-scale. The optimal T5 formulation ratio was determined be 1:1 or 3:2. Using virus ratio, filling volumes were 13–17% vial capacity. a temperature 25℃ with minimum overall time 44 h. vaccine samples prepared using these exhibited titer losses ≤ 1.0 log 10 residual...