Biocompatible Fe3O4 magnetic nanocrystals with reactive moieties on the surface can be prepared via a "one-pot" reaction and used straightforwardly in cancer detection by being coupled specific cancer-targeting antibody. It is demonstrated that as-prepared potentially as effective resonance imaging (MRI) contrast agents for diagnosis (see figure). Supporting information this article available WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c0385_s.pdf or from author. Please note: The...

Abstract Recent studies have demonstrated that gastric cancer stem cells (CSCs) are a rare sub-group of (GC) and an important role in promoting the tumor growth progression GC. In present study, we glycolytic enzyme Enolase 1 (ENO1) was involved regulation cell-like characteristics GC cells, as compared to parental cell lines PAMC-82 SNU16, expression ENO1 spheroids markedly increased. We then observed could enhance characteristics, including self-renewal capacity, invasion migration,...

Abstract Background Cancer cell is generally characterized by enhanced glycolysis. Inflammasome activation interaction with The concentration of lipopolysaccharide (LPS), a classic inflammasome activator, significantly higher in colorectal cancer tissue than normal intestinal mucosa. However, the mechanism LPS on glycolysis and metastasis has not been fully elucidated. This study aimed to investigate roles activation, glycolysis, metastasis, unravel metformin’s potential treatment CRC....

Background Focusing on antiangiogenesis may provide promising choices for treatment of gastric cancer (GC). This study aimed to investigate the mechanistic role BCAT1 in pathogenesis GC, particularly angiogenesis. Methods Bioinformatics and clinical samples analysis were used expression potential mechanism GC. BGC823 cells with overexpression or silencing induced by lentiviral transduction. Cell phenotypes angiogenesis evaluated. The relevant proteins quantized Western blotting,...

This study aimed to identify novel biological markers for the prediction of colorectal cancer liver metastasis.We established two models that mimicked interactions between tumor cells and microenvironment. From these we subcell lines had an enhanced ability metastasize liver. Genes related hepatic metastasis were screened by microarray. The candidate tested immunohistochemistry, their predictive accuracy was assessed cross-validation method independent test set.Highly metastatic colon cell...

Cancer stem cells (CSCs) are undifferentiated cancer with a high tumorigenic activity, the ability to undergo self-renewal, and multilineage differentiation potential. Clinical evidence suggests that CSCs in tumor mass cellular determinants promote invasion metastasis. MicroRNAs (miRNAs) have emerged as important modulators of cell characteristics. Unveiling candidate miRNAs regulate may provide novel therapeutic targets against cancer. We analyzed miRNA expression profiles regulating...

Gastric cancer stem cells (GCSCs) have been used as a therapeutic target. This study aims to estimate the role of miR-98-5p (termed miR-98) in development GCSCs. The expression miR-98 CD44+ GCSCs was verified by RT-PCR. overexpressed Lentivirus. ability self-renewal, invasion, chemoresistance and tumorigenicity detected vitro or vivo after overexpression miR-98. target genes were predicted luciferase reporter assays. effects miR-98/BCAT1 signaling on investigated xenograft model rescue...

Early detection of liver metastasis is important for improving colorectal cancer (CRC) patient survival. Our previous studies showed haptoglobin was highly expressed in primary CRC tissues, especially heterochronous metastatic cases. Here, we assessed the potential serum (sHP) as a biomarker early by evaluating sHP 475 patients and 152 healthy volunteers. In training set (250 cases), level CRC-M1 (1773.18 ± 690.25 ng/mL) were significantly increased compared to CRC-M0 (1544.37 1497.65 or...

Abstract The fatality rate of non-small cell lung cancer (NSCLC) has been high due to the existence stem cells (CSCs). Non-muscle myosin heavy chain 9 (MYH9) can promote progression various tumors, but its effect on cell-like characteristics (LCCs) not clarified. Our research found that stemness LCCs were significantly enhanced by overexpression MYH9, and knockout MYH9 had opposite effects. in vivo with inhibitor blebbistatin further confirmed behavior LCCs. Furthermore, western blotting...

Heat-shock protein 90 (Hsp90) plays a crucial role in tumorigenesis and tumor progression; however, its mechanism of action gastric cancer (GC) remains unclear. Here, the Hsp90 GC metabolism is focus this research. High expression tissues can interact with glycolysis, collectively affecting prognosis clinical samples. Both vitro vivo experiments demonstrate that able to regulate migration stemness properties cells. Metabolic phenotype analyses indicate influences glycolytic metabolism....

Resistance to treatment is a critical factor contributing poor prognosis in gastric cancer patients. HSP90 has emerged as promising therapeutic target; however, its role regulating tumor metabolic pathways, particularly glycolysis, remains poorly understood, which limits clinical application. We identified proteins that directly interact with using immunoprecipitation (IP) followed by mass spectrometry. The relationship between and glycolysis was further investigated through transcriptomic...

Objective To evaluate the clinical application of inferior vena cava filter (IVCF) guided by color Doppler flow imaging (CDFI) in patients with lower extremity deep vein thrombosis (DVT). Methods Thirty three extremities were treated CDFI IVCF implantation through femoral vein. The shape and position monitored ultrasound X-ray, PE complications followed up. Results 33 examined before operation. vein, common iliac bifurcation, cava, left right openings clearly displayed. All veins had no...

Abstract Introduction Aberrant microenvironment and endoplasmic reticulum (ER) stress are associated with solid-tumor progression. Stress proteins, like heat shock proteins glucose-regulated frequently overexpressed in human tumors. It has been reported that derlin-1 is involved ER response. In vitro studies have demonstrated participates the retrotranslocation of misfolded from into cytosol. Because roles cancer not yet characterized, we investigated expression breast carcinoma whether it...

Abstract Purpose: The purpose of the present study was to screen autoantibody signature colon cancers develop serum markers for cancer detection. Experimental Design: A phage cDNA expression library built. sequentially screened by a pool 10 sera, goat antihuman IgG, and two healthy sera identify phage-expressed antigens recognized tumor-associated antibodies. clones picked out these screening were subjected training set with 24 sera. antigen combination, which got most satisfactory...

Background.Carboxypeptidase A4 (CPA4) belongs to a member of the metallocarboxypeptidase family, and its expression in lung cancer samples clinical significance are still not investigated until now.In this study, we aimed evaluate level CPA4 non-small-cell (NSCLC) correlate with outcome.Methods.CPA4 gene tissues were analyzed by using Oncomine database (www.oncomine.org).The CPA4, Survivin VEGF adjacent normal evaluated IHC corresponding primary antibodies on two different commercial tissue...

Background: Alpha-enolase is an important glycolytic enzyme, and its aberrant expression has been associated with multiple tumor progression.However, few studies investigated the of alpha-enolase clinical significance in pancreatic cancer (PC).Objectives: To evaluate level PC tissues by immunohistochemical (IHC) analysis, investigate association clinicopathologic features.Methods: The levels were analyzed using Oncomine database.The alpha-enolase, Ki67 p53 adjacent normal evaluated IHC...