A5063770408- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Microbial Metabolic Engineering and Bioproduction
- Medicinal Plants and Bioactive Compounds
- Biofuel production and bioconversion
- Analytical Chemistry and Sensors
- Phytochemistry and Biological Activities
- Microfluidic and Capillary Electrophoresis Applications
- Diet, Metabolism, and Disease
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Biochemical Acid Research Studies
- Natural Antidiabetic Agents Studies
- Analytical Chemistry and Chromatography
- Phytochemical and Pharmacological Studies
- Phytochemical compounds biological activities
- Glycosylation and Glycoproteins Research
- Natural product bioactivities and synthesis
- Biochemical and biochemical processes
- Chemical Synthesis and Analysis
- Synthesis of Organic Compounds
- Flavonoids in Medical Research
Institute of Chemistry of the Slovak Academy of Sciences
2016-2025
Slovak Academy of Sciences
2016-2025
Slovak University of Technology in Bratislava
1995-2006
AREA Science Park
1999
Vitenparken
1999
Université Le Havre Normandie
1998
Phenylethanoid glycosides (PhGs) are widely occurring secondary metabolites of medicinal plants with interesting biological activities such as antioxidant, anti-inflammatory, neuroprotective, antiviral, hepatoprotective, immunomodulatory, etc. They characterized by a structural core formed phenethyl alcohol, usually tyrosol or hydroxytyrosol, attached to β-D-glucopyranose via glycosidic bond. This is further decorated phenolic acids another saccharide. Several studies suggest an important...
A commercial glycosidase mixture obtained from Penicillium multicolor (Aromase H2) was found to comprise a specific diglycosidase activity, β-acuminosidase, alongside undetectable levels of β-apiosidase. The enzyme tested in the transglycosylation tyrosol using 4-nitrophenyl β-acuminoside as diglycosyl donor. reaction not chemoselective, providing Osmanthuside H and its counterpart regioisomer 4-(2-hydroxyethyl)phenyl 58% yield. Aromase H2 is therefore first β-acuminosidase which also able...
Various chemoenzymatic routes to optically pure biologically active dibydropyridines are reviewed.The review is focused on influence the biotransformation of main in vivo enzymatic activity hydrolase (lipase, esterase, protease), microorganism source lipase and substituents I,4-dihydropyridine ring, as well physicochemical properties solvent nature nucleophile.The these variables desymmetrisation prochiral diesters or resolution racemic compounds analyzed.
Tyrosol β-d-fructofuranoside and hydroxytyrosol have been synthesized as new compounds in 27.6 19.5% respective yields through transfructosylation of tyrosol hydroxytyrosol. Yeast β-galactosidase Lactozym 3000 L comprising invertase activity was used catalyst. Besides the main monofructosides, an equimolar mixture β-d-fructofuranosyl-((2→1)-β-d-fructofuranoside β-d-fructofuranosyl-(2→6)-β-d-fructofuranoside isolated additional product fraction 14.3% yield.
Fungal β-galactosidase from Aspergillus oryzae was immobilized into polyvinylalcohol (PVA) hydrogel by LentiKats® technology and used for the production of short-chain alkyl glycosides. Ethyl- propyl-β-d-galactopyranosides were prepared lactose (100 g/L) varying initial amounts alcohol (10–30% v/v) at 40 °C pH 4.5. The entrapped preserved 50% transgalactosylation activity after 25 repeated cycles in ethyl β-d-galactopyranoside. When 5% (v/v) propanol as an acceptor, enzyme (30–32 U/g enzyme)...
Apiose is a branched pentose naturally occurring either as component of the plant cell wall polysaccharides or sugar moiety present in numerous secondary metabolites such flavonoid and phenylethanoid glycosides, substrates defense systems glycosylated aroma precursors. The enzymes catalyzing hydrolysis apiosylated substances (mainly glycosidases specific towards apiose acuminose) have promising applications not only (flavor development), but potentially also synthesis apiosides...
Four novel active esters of 4-hydroxy-3-methoxycinnamic acid as substrates for feruloyl esterases were prepared in a four-step procedure including chemoselective enzymatic deprotection acetylated compounds by Lipase PS.