A5009372470- Listeria monocytogenes in Food Safety
- Essential Oils and Antimicrobial Activity
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Brucella: diagnosis, epidemiology, treatment
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Probiotics and Fermented Foods
- Nosocomial Infections in ICU
- Gastrointestinal motility and disorders
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
Université Paris Cité
2021-2024
Institut Pasteur
2017-2024
Centre National de la Recherche Scientifique
2021-2024
Universidad de Costa Rica
2018-2023
Sorbonne Paris Cité
2021
Inserm
2017-2018
Listeria monocytogenes is a Gram-positive food-borne pathogen that in humans may traverse the intestinal, placental and blood/brain barriers, causing gastroenteritis, abortions meningitis. Crossing of these barriers dependent on bacterial ability to enter host cells, several L. surface secreted virulence factors are known facilitate entry intracellular lifecycle. The study strains associated human listeriosis epidemics has revealed presence novel factors. One such factor Listeriolysin S,...
ABSTRACT Streptolysin S (SLS)-like virulence factors from clinically relevant Gram-positive pathogens have been proposed to behave as potent cytotoxins, playing key roles in tissue infection. Listeriolysin (LLS) is an SLS-like hemolysin/bacteriocin present among Listeria monocytogenes strains responsible for human listeriosis outbreaks. As LLS cytotoxic activity has associated with virulence, we investigated the LLS-specific contribution host Surprisingly, first show that causes only weak...
Brucella abortus is a facultative extracellular-intracellular pathogen belonging to group of Alphaproteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in membrane-bound compartment, avoiding the lysosomal route and reaching endoplasmic reticulum through action type IV secretion system, VirB. In this work, we demonstrate BvrR/BvrS two-component system senses intracellular environment mount transcriptional response required for life adaptation....
ObjectivesTo investigate the contribution to virulence of surface protein internalin B (InlB) in Listeria monocytogenes lineage I strain F2365, which caused a deadly listeriosis outbreak California 1985.MethodsThe F2365 displays point mutation that hampers expression InlB. We rescued InlB L. by introducing codon 34 (TAA CAA). investigated its importance for bacterial using vitro cell infection systems and murine intravenous model.ResultsIn HeLa JEG-3 cells, InlB+ expressing was ≈9-fold...
Significance Listeria monocytogenes ( Lm ) is a bacterial pathogen that causes listeriosis, foodborne disease characterized by gastroenteritis, meningitis, bacteremia, and abortions in pregnant women. The most severe human listeriosis outbreaks are associated with subset of hypervirulent clones encode the bacteriocin Listeriolysin S (LLS), which modifies gut microbiota allows efficient colonization invasion deeper organs. Our present work identifies killing mechanism displayed LLS to...
Clinical symptoms of Clostridioides difficile infection (CDI) range from diarrhea to pseudomembranous colitis. A major challenge in managing CDI is the high rate relapse. Several studies correlate production CDT binary toxin by clinical strains C. with higher relapse rates. Although mechanism action on host cells known, its exact contribution still unclear. To understand physiological role during CDI, we established two hypoxic relevant intestinal models, Transwell and Microfluidic...
Background Listeria monocytogenes is a foodborne bacterial pathogen that causes listeriosis, an infectious disease in animals and people, with pigs acting as asymptomatic reservoirs. In August 2019 outbreak associated the consumption of pork meat caused 222 human cases listeriosis Spain. Determining diversity well virulence potential strains from important to public health. Methods The behaviour 23 L recovered pig tonsils, skin was compared by studying (1) internalin A, B, listeriolysin O,...
Brucella abortus is a zoonotic pathogen whose virulence depends on its ability to survive intracellularly at the endoplasmic reticulum derived compartment. The two-component system BvrR/BvrS (BvrRS) essential for intracellular survival due transcriptional control of type IV secretion VirB and regulator VjbR. It master several traits including membrane homeostasis by controlling gene expression components, such as Omp25. BvrR phosphorylation related DNA binding target regions, thereby...
Alphaproteobacteria include organisms living in close association with plants or animals. This interaction relies partly on orthologous two-component regulatory systems (TCS), sensor and regulator proteins modulating the expression of conserved genes related to symbiosis/virulence. We assessed ability exoS+Sm gene, encoding a protein from plant endosymbiont Sinorhizobium meliloti substitute its bvrS animal/human pathogen Brucella abortus. ExoS phosphorylated B. abortus BvrR vitro cultured...