A5009526654- Brucella: diagnosis, epidemiology, treatment
- Escherichia coli research studies
- Burkholderia infections and melioidosis
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Galectins and Cancer Biology
- Plant Pathogenic Bacteria Studies
- Cocoa and Sweet Potato Agronomy
- Phytoplasmas and Hemiptera pathogens
- Atherosclerosis and Cardiovascular Diseases
- Zoonotic diseases and public health
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Animal Diversity and Health Studies
- Monoclonal and Polyclonal Antibodies Research
- Aquaculture disease management and microbiota
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Celiac Disease Research and Management
- Microbial infections and disease research
- Yersinia bacterium, plague, ectoparasites research
- Leptospirosis research and findings
- Phagocytosis and Immune Regulation
- Rabies epidemiology and control
Universidad de Costa Rica
2015-2024
Universidad Nacional
2007-2017
Wellcome Sanger Institute
2017
University of Liverpool
2017
Costa Rican Department of Social Security
2017
University of Bari Aldo Moro
2017
Institute for Sustainable Plant Protection
2017
To unravel the strategy by which Brucella abortus establishes chronic infections, we explored its early interaction with innate immunity.Brucella did not induce proinflammatory responses as demonstrated absence of leukocyte recruitment, humoral or cellular blood changes in mice. hampered neutrophil (PMN) function and PMN depletion influence course infection. barely induced cytokines consumed complement, was strongly resistant to bactericidal peptides, extracts serum. LPS (BrLPS),...
Innate immunity recognizes bacterial molecules bearing pathogen-associated molecular patterns to launch inflammatory responses leading the activation of adaptive immunity. However, lipopolysaccharide (LPS) gram-negative bacterium Brucella lacks a marked pattern, and it has been postulated that this delays development immunity, creating gap is critical for reach intracellular replicative niche. We found B. abortus mutant in wadC gene displayed disrupted LPS core while keeping both...
Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, group taxonomists merged brucellae with primarily free-living, phylogenetically related
Xylella fastidiosa is a plant-pathogenic bacterium recently introduced in Europe that causing decline olive trees the South of Italy. Genetic studies have consistently shown bacterial genotype recovered from infected belongs to sequence type ST53 within subspecies pauca. This genotype, ST53, has also been reported occur Costa Rica. The ancestry was clarified, showing it contains alleles are monophyletic with those subsp. pauca America. To more robustly determine phylogenetic placement X....
Background During evolution, innate immunity has been tuned to recognize pathogen-associated molecular patterns. However, some α-Proteobacteria are stealthy intracellular pathogens not readily detected by this system. Brucella members follow strategy and highly virulent, but other Brucellaceae like Ochrobactrum rhizosphere inhabitants only opportunistic pathogens. To gain insight into the emergence of strategy, we compared these two phylogenetically close biologically divergent bacteria....
Brucella ceti infections have been increasingly reported in cetaceans. Brucellosis these animals is associated with meningoencephalitis, abortion, discospondylitis', subcutaneous abscesses, endometritis and other pathological conditions B. frequently described dolphins from both, the Atlantic Pacific Oceans. In Mediterranean Sea, only two reports made: one Italian Tyrrhenian Sea Adriatic Sea. We describe clinical features of three cases stranded Catalonian coast. One striped dolphin had...
Abstract Several species of Brucella are known to be zoonotic, but B. neotomae infection has been thought limited wood rats. In 2008 and 2011, however, was isolated from cerebrospinal fluid 2 men with neurobrucellosis. The nonzoonotic status should reassessed.
Most bacterial infections induce the activation of polymorphonuclear neutrophils (PMNs), enhance their microbicidal function, and promote survival these leukocytes for protracted periods time. Brucella abortus is a stealthy pathogen that evades innate immunity, barely activates PMNs, resists killing mechanisms phagocytes. Intriguing clinical signs observed during brucellosis are low numbers infected PMNs in target organs neutropenia proportion patients; features deserve further attention....
Canine brucellosis caused by Brucella canis is a disease of dogs and zoonotic risk. B. harbors most the virulence determinants defined for genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium an intracellular pathogen. Studies outbreaks in kennel facilities indicated infected displaying clinical signs did present hematological alterations. A virulent strain isolated from those readily replicated different organs mice...
Brucella abortus is a facultative extracellular-intracellular pathogen belonging to group of Alphaproteobacteria that establishes close interactions with animal cells. This bacterium enters host cells in membrane-bound compartment, avoiding the lysosomal route and reaching endoplasmic reticulum through action type IV secretion system, VirB. In this work, we demonstrate BvrR/BvrS two-component system senses intracellular environment mount transcriptional response required for life adaptation....
Intracellular bacterial pathogens probably arose when their ancestor adapted from a free-living environment to an intracellular one, leading clonal bacteria with smaller genomes and less sources of genetic plasticity. Still, this plasticity is needed respond the challenges posed by host. Members Brucella genus are facultative-extracellular responsible for causing brucellosis in variety mammals. The various species keep different host preferences, virulence, zoonotic potential despite having...
Brucellosis is a bacterial infectious disease affecting wide range of mammals and neglected zoonosis caused by species the genetically homogenous genus Brucella. As in most studies on diseases, research brucellosis carried out using reference strains as canonical models to understand mechanisms underlying host pathogen interactions. We performed whole genome sequencing (WGS) analysis strain Brucella abortus 2308 routinely used our laboratory, including manual curated annotation accessible an...
Brucella abortus is a stealthy intracellular bacterial pathogen of animals and humans. This bacterium promotes the premature cell death neutrophils (PMN) resists killing action these leukocytes. B. abortus-infected PMNs presented phosphatidylserine (PS) as "eat me" signal on surface. promoted direct contacts between macrophages (Mϕs) favored phagocytosis infected dying PMNs. Once inside Mϕs, replicated within Mϕs at significantly higher numbers than when were with bacteria alone. The high...
Desmodus rotundus, vampire bats, transmit dangerous infections, and brucellosis is a hazardous zoonotic disease, two adversities that coexist in the subtropical tropical areas of American continent. Here, we report 47.89% Brucella infection prevalence colony bats inhabiting rainforest Costa Rica. The bacterium induced placentitis fetal death bats. Wide-range phenotypic genotypic characterization placed organisms as new pathogenic species named nosferati sp. nov., isolated from bat tissues,...
The brucellae are α-Proteobacteria facultative intracellular parasites that cause an important zoonosis. These bacteria escape early detection by innate immunity, ability associated to the absence of marked pathogen-associated molecular patterns in cell envelope lipopolysaccharide, lipoproteins and flagellin. We show here that, contrast outer membrane ornithine lipids (OL) other Gram negative bacteria, Brucella abortus OL lack a pattern activity. identified two genes (olsB olsA) generating...
Brucellosis, caused by Brucella abortus is a major disease of cattle and zoonosis. In order to estimate the bovine brucellosis prevalence in Costa Rica (CR), total 765 herds (13078 bovines) from six regions CR were randomly sampled during 2012–2013. A non-random sample 7907 (532199 regions, arriving for diagnoses 2014–2016 Rican Animal Health Service was also studied. The estimated Rose Bengal test (RBT) ranged 10.5%-11.4%; alternatively, testing RBT positives iELISA, 4.1%-6.0%,...
Abstract Background Pathogens with a global distribution face diverse biotic and abiotic conditions across populations. Moreover, the ecological evolutionary history of each population is unique. Xylella fastidiosa xylem-dwelling bacterium infecting multiple plant hosts, often detrimental effects. As group, X. divided into distinct subspecies allopatric historical distributions patterns introductions from numerous source The capacity to successfully colonize cause disease in naïve hosts...
Brucellosis has been an endemic disease of cattle and humans in Costa Rica since the beginning XX century. However, brucellosis sheep, goats, pigs, water buffaloes, horses cetaceans, not reported country. We have performed a survey these host mammal species, from 1999–2016. In addition, we documented number human cases, 2003–2016. The seroprevalence goat sheep herds was 0.98% 0.7% respectively, with no Brucella isolation. Antibodies against were detected feral or domestic pigs. Likewise,...
Brucellosis is a chronic bacterial disease caused by members of the genus Brucella. Among classical species stands Brucella neotomae, until now, pathogen limited to wood rats. However, we have identified two brucellosis human cases B. demonstrating that this has zoonotic potential.Within almost 4 years each other, 64-year-old Costa Rican white Hispanic man and 51-year-old required medical care at public hospitals Rica. Their hematological biochemical parameters were within normal limits. No...
Brucellosis, caused by Brucella abortus, is a major disease of cattle and humans worldwide distributed. Eradication control the has been difficult in Central South America, Asia, Mediterranean Middle East. Epidemiological strategies combined with phylogenetic methods provide high-resolution power needed to study relationships between surveillance data pathogen population dynamics, using genetic diversity spatiotemporal distributions. This information crucial for prevention spreading at local...
Bovine brucellosis induces abortion in cows, produces important economic losses, and causes a widely distributed zoonosis. Its eradication was achieved several countries after sustained vaccination with the live attenuated Brucella abortus S19 vaccine, combination slaughtering of serologically positive animals. antibodies against smooth lipopolysaccharide (S-LPS), making difficult differentiation infected from vaccinated bovines. We developed an strain constitutively expressing green...
Members of the genus Brucella cluster in two phylogenetic groups: classical and non-classical species. The former group is composed species that cause disease mammals, including humans. A species, labeled as sp. BCCN84.3, was isolated from testes a Saint Bernard dog suffering orchiepididymitis, Costa Rica. Following standard microbiological methods, bacterium first defined "Brucella melitensis biovar 2." Further molecular typing, identified strain an atypical suis." Distinctive BCCN84.3...
Brucella abortus is a facultatively extracellular-intracellular pathogen that encounters diversity of environments within the host cell. We report bacteria extracted from infected cells at late stages (48 h postinfection) intracellular life cycle significantly increase their ability to multiply in new target cells.