A5067225986- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Osteoarthritis Treatment and Mechanisms
- Ubiquitin and proteasome pathways
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Developmental Biology and Gene Regulation
- Genetic Neurodegenerative Diseases
- 14-3-3 protein interactions
- Plant Molecular Biology Research
- Hippo pathway signaling and YAP/TAZ
- Bone Tumor Diagnosis and Treatments
- Cancer Treatment and Pharmacology
- Congenital heart defects research
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Reproductive Biology
- Cardiomyopathy and Myosin Studies
- RNA regulation and disease
- Protein Kinase Regulation and GTPase Signaling
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Photosynthetic Processes and Mechanisms
- biodegradable polymer synthesis and properties
- Fetal and Pediatric Neurological Disorders
Université Paris-Saclay
2016-2025
Inserm
2016-2025
Physiopathologie et Epidémiologie des Maladies Respiratoires
2019
Université Paris-Sud
2016-2018
Centre National de la Recherche Scientifique
2005-2014
Université Paris Cité
2012-2014
Délégation Paris 7
2012-2014
Institut Jacques Monod
1999-2012
Sorbonne Paris Cité
2012
University of Massachusetts Chan Medical School
2009
Genetic control of embryogenesis switches from the maternal to zygotic genome during maternal-to-zygotic transition (MZT), when mRNAs are destroyed, high-level transcription is initiated, replication checkpoint activated and cell cycle slows. The midblastula transition(MBT) first morphological event that requires gene expression. Drosophila MBT marked by blastoderm cellularization follows 13 cleavage-stage divisions. RNA-binding protein Smaug required for cleavage-independent transcript...
Abstract Cancer cell resistance to taxanes is a complex, multifactorial process, which results from the combination of several molecular and cellular changes. In breast cancer cells adapted long-term paclitaxel treatment, we previously identified new adaptive mechanism that contributes involves high levels tubulin tyrosination long-chain polyglutamylation coupled with septin expression, especially SEPT9_i1. This in turn led higher CLIP-170 MCAK recruitment microtubules enhance microtubule...
The poly(A)-binding protein II (PABP2) is one of the polyadenylation factors required for proper 3′-end formation mammalian mRNAs. We have cloned Pabp2, gene encoding Drosophila homolog PABP2, by using a molecular screen to identify new proteins with RNP-type RNAbinding domains. Sequence comparison PABP2 from and mammals indicates that most conserved domains are RNA-binding domain coiled-coil like which could be involved in protein-protein interactions. Pabp2 produces four mRNAs result...
Microtubules (MTs) are essential for cell division, shape, intracellular transport, and polarity. MT stability is regulated by many factors, including MT-associated proteins controlling the amount of free tubulin heterodimers available polymerization. Tubulin-binding cofactors potential key regulators concentration, since they required α-β–tubulin dimerization in vitro. In this paper, we show that mutation Drosophila tubulin-binding cofactor B (dTBCB) affects levels both α- β-tubulins...
The stress-induced c-Jun N-terminal kinase (JNK) controls microtubule dynamics by enhancing both growth and rescues. Here, we show that upon cell stress, JNK directly phosphorylates the rescue factor CLIP-170 in its microtubule-binding domain to increase rescue-promoting activity. Phosphomimetic versions of enhance ability promote events vitro cells. Furthermore, while phosphomimetic mutations do not alter CLIP-170’s capability form comets at growing ends, activation occurrence remnants on...
Abstract Meningomyelocele (MM) is considered a genetically complex disease resulting from failure of neural tube closure (NTD). Patients display neuromotor disability and frequent hydrocephalus requiring ventricular shunting. A few proposed genes contribute to susceptibility, but most risk remains unexplained 1 . We postulated that de novo mutations (DNMs) under purifying selection MM 2 Here we recruited cohort 851 trios shunting at birth, compared with 732 control trios, found likely gene...
The Suppressor of forked protein is the Drosophila homolog 77K subunit human cleavage stimulation factor, a complex required for first step mRNA 3'-end-processing reaction. We have shown previously that wild-type su(f) function accumulation truncated transcript polyadenylated in intron 4 gene. This led us to propose model which Su(f) would negatively regulate its own by stimulating 3'-end formation this RNA. In article, we demonstrate and show autoregulation tissue specific. accumulates at...
The Suppressor of forked [Su(f)] protein is the Drosophila homologue CstF-77, a subunit human cleavage stimulation factor (CstF) that required for first step mRNA 3′ end processing reaction in vitro . We have addressed directly role su(f) vivo show pre-mRNA during formation. Analysis functional complementation between Su(f) and CstF-77 shows most (85%) can be exchanged to produce chimeric CstF-77/Su(f) proteins rescue lethality defect formation mutants. Interestingly, we domain limiting this...
Human articular cartilage cells were transfected with the t.-sensitive polyomavirus large T antigen of SV40. Several immortalized chondrocyte cell lines obtained. The types acidic polysaccharides and collagen synthesized suggest dedifferentiation in vitro culture system used afterwards to obtain numbers cells.