A5048692725- Osteoarthritis Treatment and Mechanisms
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Lipid metabolism and disorders
- Hepatitis C virus research
- Endoplasmic Reticulum Stress and Disease
- Tendon Structure and Treatment
- Diet, Metabolism, and Disease
- Pancreatic function and diabetes
- Advanced Proteomics Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cholesterol and Lipid Metabolism
- Skin and Cellular Biology Research
- Protein purification and stability
- Diet and metabolism studies
- Blood properties and coagulation
- Silk-based biomaterials and applications
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- RNA Interference and Gene Delivery
- Gut microbiota and health
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
Inserm
2003-2025
École Pratique des Hautes Études
2004-2025
Université Paris Sciences et Lettres
2020-2025
Centre de Recherche Saint-Antoine
2017-2025
Sorbonne Université
2003-2020
Université Paris Cité
2010-2020
Centre de Recherche des Cordeliers
1990-2020
Centre National de la Recherche Scientifique
2020
Délégation Paris 5
2011-2020
Sorbonne Paris Cité
2020
Chondrocytes cultivated in monolayer rapidly divide and lose their morphological biochemical characteristics, whereas they maintain phenotype for long periods of time when are alginate beads. Because cartilage has a low cellularity is difficult to obtain large quantities, the number available cells often becomes limiting factor studies chondrocyte biology. Therefore, we explored possibility restoring differentiated properties chondrocytes by cultivating them beads after two multiplication...
Intestinal absorption of alimentary lipids is a complex process ensured by enterocytes and leading to TRL [TAG (triacylglycerol)-rich lipoprotein] assembly secretion. The accumulation circulating intestine-derived associated with atherosclerosis, stressing the importance control postprandial hypertriglyceridaemia. During period, TAGs are also transiently stored as CLDs (cytosolic lipid droplets) in enterocytes. As first step for determining whether could play role enterocyte secretion, we...
Enterocytes, the intestinal absorptive cells, have to deal with massive alimentary lipids upon food consumption. They orchestrate complex lipid-trafficking events that lead secretion of triglyceride-rich lipoproteins and/or intracellular transient storage as lipid droplets (LDs). LDs originate from endoplasmic reticulum (ER) membrane and are mainly composed a triglyceride (TG) cholesterol-ester core surrounded by phospholipid cholesterol monolayer specific coat proteins. The pivotal role in...
Hepatitis C virus (HCV) in cell culture has a density comparable to that of other members the family Flaviviridae , whereas vivo infectious particles are found partially low-density fractions, associated with triacylglycerol (TG)-rich lipoproteins (TRLs). In blood infected patients, HCV circulates as heterogeneous particles, among which lipo-viroparticles (LVPs), globular rich TG and containing viral capsid RNA. The dual lipoprotein nature LVPs was addressed further respect apolipoprotein...
Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids Cell culture) was used explore the lipopolysaccharide (LPS) effects including LPS‐induced oxidation cascades on a dedicated intestinal epithelial cell line (HT29‐MD2) together with...
Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids Cell culture) was used explore the lipopolysaccharide (LPS) effects including LPS‐induced oxidation cascades on a dedicated intestinal epithelial cell line (HT29‐MD2) together with...
Inflammatory bowel diseases (IBD), which include Crohn's disease and ulcerative colitis represent a global health issue as prevalence of 1% is expected in the western world by end this decade. These idiseases are associated with high oxidative stress that induces inflammatory pathways severely damages gut tissues. IBD patients suffer from antioxidant defenses weakening, through, for instance, an impaired activity superoxide dismutases (SOD) — catalyze dismutation or other endogenous enzymes...
Decrease of plasma lipid levels by polyphenols was linked to impairment hepatic lipoprotein secretion. However, the intestine is first epithelium that faces dietary compounds, and it contributes homeostasis secreting triglyceride-rich lipoproteins during postprandial state. The purpose this study examine effect apple wine polyphenol extracts on synthesis secretion in human Caco-2/TC7 enterocytes apically supplied with complex micelles. Our results clearly demonstrate apple, but not wine,...
In enterocytes, the dynamic accumulation and depletion of triacylglycerol (TAG) in lipid droplets (LD) during fat absorption suggests that cytosolic LD-associated TAG contribute to TAG-rich lipoprotein (TRL) production. To get insight into mechanisms controlling storage/secretion balance TAG, we used as a tool hepatitis C virus core protein, which localizes onto LDs, thus may modify their protein coat decrease TRL secretion. We compared proteome LD fractions isolated from Caco-2/TC7...
The worldwide prevalence of metabolic diseases is increasing, and there are global recommendations to limit consumption certain nutrients, especially saturated lipids. Insulin resistance, a common trait occurring in obesity type 2 diabetes, associated with intestinal lipoprotein overproduction. However, the mechanisms by which intestine develops insulin resistance response lipid overload remain unknown. Here, we show that inhibits triglyceride secretion microsomal transfer protein expression...
Abstract Cell lines were established from rabbit articular chondrocytes following transfection with a plasmid encoding SV40 early function genes. This resulted in cell immortalization (130 passages have been completed for the oldest line) acquisition of characteristics partial transformation such as reduced serum requirements normal and clonal growth. The immortalized chondrocytes, called SVRAC, did not form multilayer foci when maintained postconfluent culture. Their ability to colonies...
Intestinal triglyceride-rich lipoproteins (TRL) are synthesized from dietary lipids. This study was designed to evaluate the effects of lipid micelles, mimicking post-digestive duodenal on fate apolipoprotein B (apoB)48-containing by Caco-2 cells. Such consisting oleic acid (OA), taurocholate, 2-monooleoylglycerol (2-MO), cholesterol (Chol), and L-alpha-lysophospatidylcholine, were most efficient inducers OA uptake esterification. The efficiency TG apoB48 secretion increased specifically as...
Objective HCV is intimately linked with the liver lipid metabolism, devoted to efflux of triacylglycerols stored in droplets (LDs) form triacylglycerol-rich very-low-density lipoproteins (VLDLs): (i) most infectious particles are those lowest density due association and (ii) HCV-infected patients frequently develop hepatic steatosis (increased triacylglycerol storage). The recent identification lysophosphatidylcholine acyltransferase 1 (LPCAT1) as an LD phospholipid-remodelling enzyme...
Oxidative stress is known to play a major role in the pathogenesis of inflammatory bowel diseases (IBDs), and, particular, superoxide dismutase (SODs) defenses were shown be weakened patients suffering from IBDs. SOD mimics, also called mimetics, as low-molecular-weight complexes reproducing activity SOD, constitute promising antioxidant catalytic metallodrugs context A Mn(II) complex mimic (Mn1) based on an open-chain diaminoethane ligand exerting and anti-inflammatory effects intestinal...
Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from apical to basolateral pole. Apolipoprotein B (apoB) is a secretory protein plays key role in transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation control apoB traffic by lipids therefore particular interest. To get dynamic insight into this process, we used enterocytic Caco-2 cultured on microporous filters, system which and basal compartments can be delimited....
Enterocytes are responsible for the absorption of dietary lipids, which involves TRL [TG (triacylglycerol)-rich lipoprotein] assembly and secretion. In present study, we analysed effect on secretion Caco-2 enterocyte adaptation to a differential glucose supply. We showed that TG in cells adapted low supply 2 weeks after confluence was double control maintained high-glucose-containing medium, whereas level synthesis remained similar both conditions. This increased resulted mainly from an...