A5000201048- Lanthanide and Transition Metal Complexes
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Magnetism in coordination complexes
- Hematopoietic Stem Cell Transplantation
- Shoulder Injury and Treatment
- Molecular Sensors and Ion Detection
- Mesenchymal stem cell research
- Shoulder and Clavicle Injuries
- Metal-Catalyzed Oxygenation Mechanisms
- Nerve Injury and Rehabilitation
- Radioactive element chemistry and processing
- Metal complexes synthesis and properties
- Advanced Proteomics Techniques and Applications
- Asymmetric Hydrogenation and Catalysis
- Molecular Biology Techniques and Applications
- Trace Elements in Health
- Alzheimer's disease research and treatments
- Electron Spin Resonance Studies
- Redox biology and oxidative stress
- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Phosphorus compounds and reactions
- Globalization and Cultural Identity
- Cultural Insights and Digital Impacts
Université Paris Sciences et Lettres
2016-2025
Laboratoire des Biomolécules
2017-2025
Sorbonne Université
1984-2025
École Normale Supérieure - PSL
2016-2025
Centre National de la Recherche Scientifique
2015-2025
Centre Hospitalier Universitaire de Toulouse
2023
Hôpital Paule de Viguier
2021-2023
Université Toulouse III - Paul Sabatier
2023
Université de Toulouse
2023
Uppsala University
2018-2022
Abstract Background Recent studies have suggested that ex vivo expansion of autologous hematopoietic cells could be a therapy choice for the treatment bone marrow failure. We investigated potential combined infusion expanded with mesenchymal (MSCs) multi‐organ failure syndrome following irradiation in non‐human primate model. Methods Hematopoietic and MSCs were from aspirates. transduced gene encoding green fluorescent protein (e‐GFP), order to track them infusion. Twelve animals studied....
A series of multi-target-directed ligands are described that bind Cu, act as antioxidants, modulate Aβ peptide aggregation, and abolish toxicity in primary neurons.
Coordination complexes and supramolecules based on trivalent lanthanides for probing biological systems, in particular intracellular environments, are reviewed.
The quenching of sensitized Eu(III) luminescence by photoinduced electron transfer from the excited light-harvesting antenna to was investigated. A series complexes incorporating different metal binding sites and thus having varying Eu(III)/Eu(II) reduction potentials were prepared. fully characterized using a combination single-crystal X-ray crystallography paramagnetic 1H NMR spectroscopy, results which support structural similarity complexes. redox photophysical behavior center studied...
Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids Cell culture) was used explore the lipopolysaccharide (LPS) effects including LPS‐induced oxidation cascades on a dedicated intestinal epithelial cell line (HT29‐MD2) together with...
Overproduction of reactive oxygen species and antioxidant superoxide dismutases (SOD1, SOD2) dysregulation contribute to chronic inflammation such as generated in inflammatory bowel diseases (IBD). A kinetic redox shotgun proteomic strategy (OcSILAC for Oxidized cysteine Stable Isotope Labelling by Amino acids Cell culture) was used explore the lipopolysaccharide (LPS) effects including LPS‐induced oxidation cascades on a dedicated intestinal epithelial cell line (HT29‐MD2) together with...
Inflammatory bowel diseases (IBD), which include Crohn's disease and ulcerative colitis represent a global health issue as prevalence of 1% is expected in the western world by end this decade. These idiseases are associated with high oxidative stress that induces inflammatory pathways severely damages gut tissues. IBD patients suffer from antioxidant defenses weakening, through, for instance, an impaired activity superoxide dismutases (SOD) — catalyze dismutation or other endogenous enzymes...
Inorganic complexes are increasingly used for biological and medicinal applications, the question of cell penetration distribution metallodrugs is key to understanding their activity. Oxidative stress known be involved in inflammation inflammatory bowel diseases which antioxidative defenses weakened. We report here study manganese complex Mn1 mimicking superoxide dismutase (SOD), a protein protection against oxidative stress, using an approach inorganic cellular chemistry combining...
Sulfated polyanions, including pentosan polysulfate (PPS) and heparan mimetics, number among the most effective drugs that have been used in experimental models of prion disease are presumed to act competition with endogenous sulfate proteoglycans as co-receptors for protein (PrP) on cell surface. PPS has shown prolong survival animals after intracerebral perfusion is limited use treatment human transmissible spongiform encephalopathies (TSEs). Here, compared CR36, a new mimetic. Ex vivo ,...
Superoxide dismutases (SODs) are highly efficient enzymes for superoxide dismutation and the first line of defense against oxidative stress. These metalloproteins contain a redox-active metal ion in their active site (Mn, Cu, Fe, Ni) with tightly controlled reduction potential found close range around optimal value 0.36 V versus normal hydrogen electrode (NHE). Rationally designed proteins well-defined three-dimensional structures offer new opportunities obtaining functional SOD mimics....
Cupredoxins are copper-dependent electron-transfer proteins that can be categorized as blue, purple, green, and red depending on the spectroscopic properties of Cu(II) bound forms. Interestingly, despite significantly different first coordination spheres nuclearity, all cupredoxins share a common Greek Key β-sheet fold. We have previously reported design copper protein within completely distinct three-helical bundle protein, α3DChC2.(1) While this demonstrated β-barrel fold was not requisite...
A superoxide dismutase mimic (Mn1) was functionalized with three positively charged-peptides: RRRRRRRRR (Mn1-R9), RRWWWRRWRR (Mn1-RW9) or Fx-r-Fx-K (Mn1-MPP). Characterization of the physico-chemical properties complexes show that they share similar binding affinity for Mn2+, apparent reduction potential and intrinsic activity. However, their accumulation in cells is different (Mn1-R9 < Mn1-MPP Mn1-RW9 Mn1), as well subcellular distribution. In addition, functionalized-complexes display a...
The study of Mn-based superoxide dismutase mimic conjugated with a multimodal Re-probe in cellular model oxidative stress revealed that its bioactivity is associated accumulation at the mitochondria.
Yb(III) complexes of macrocyclic ligands based on 1,4,7,10-tetraazacyclododecane were synthesized. The carried a carbostyril chromophore for sensitization, and carboxylate or carbamide donors metal binding, forming 0, +1, +2, +3 overall charge. coordination geometry was little affected by the replacement carboxylates with amides, as shown paramagnetic 1H NMR spectroscopy. Yb(III)/Yb(II) reduction potentials dependent nature binding site, more positively charged easier to reduce. Carbostyril...
The coordination compounds of the trivalent lanthanide ions (Ln(III)) have unique photophysical properties. Ln(III) excitation is usually performed through a light-harvesting antenna. To enable Ln(III)-based emitters to reach their full potential, an understanding how complex structure affects sensitization and quenching processes necessary. Here, role linker between antenna metal binding fragment was studied. Four macrocyclic ligands carrying coumarin 2 or 4-methoxymethylcarbostyril...
To assess the therapeutic efficacy of ex vivo-expanded hematopoietic cells in treatment radiation-induced pancytopenia, we have set up a non-human primate model. Two vivo expansion protocols for bone marrow mononuclear (BMMNC) were studied. The first consisted 7-day culture presence stem cell factor (SCF), Flt3-ligand, thrombopoietin (TPO), interleukin-3 (IL-3), and IL-6, which induced preferentially immature [3.1 +/- 1.4, 10.0 5.1, 2.2 1.9, 1.0 0.3-fold (MNC), colony-forming...
The progressive, neurodegenerative Alzheimer's disease (AD) is the most widespread dementia. Due to ageing of population and current lack molecules able prevent or stop disease, AD will be even more impactful for society in future. a multifactorial and, among other factors, metal ions have been regarded as potential therapeutic targets. This case redox-competent Cu involved production reactive oxygen species (ROS) when bound Alzheimer-related Aβ peptide, process that contributes overall...
Purpose: To define the ability of bone marrow mononuclear cells (BMMNC) to expand after irradiation and determine amount apoptosis in irradiated expanded cells.Materials methods: Non‐human primate BMMNC were vitro at doses ranging from 0 4 Gy cultured during 1 week presence interleukin 3, 6, stem cell factor, thrombopoietin fms‐like tyrosine kinase‐3 ligand. The expansion yield BMMNC, colony‐forming CD34+ compared with non‐irradiated control cultures. Apoptosis was also defined by annexin...
Yb(III) complexes based on ligands with a 1,4,7-triazacyclononane (tacn) macrocyclic core were synthesised. The carry 4-methoxymethyl-substituted carbostyril chromophore that serves as light-harvesting antenna. supply 5 nitrogen and 3 oxygen donors via 1 methylenecarboxamide 2 picolinate donors, creating +1 charged an octadentate binding environment. electronic properties of the picolinates are modulated by varying substitution at 4 position OMe, H, Cl, or CF3. Cyclic voltammetry indicated...