A5001864530- Synthesis and Reactions of Organic Compounds
- Synthesis and Reactivity of Heterocycles
- Synthesis of heterocyclic compounds
- Synthesis and Biological Evaluation
- Plant-derived Lignans Synthesis and Bioactivity
- Asymmetric Synthesis and Catalysis
- Synthesis and biological activity
- Traditional and Medicinal Uses of Annonaceae
- Biological Activity of Diterpenoids and Biflavonoids
- Bioactive Natural Diterpenoids Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and Characterization of Pyrroles
- Plant biochemistry and biosynthesis
- Synthesis and Characterization of Heterocyclic Compounds
- Phytochemistry and Biological Activities
- Venomous Animal Envenomation and Studies
- Oxidative Organic Chemistry Reactions
- Essential Oils and Antimicrobial Activity
- Toxin Mechanisms and Immunotoxins
- Synthetic Organic Chemistry Methods
- Psidium guajava Extracts and Applications
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Medicinal Plant Extracts Effects
- Magnetism in coordination complexes
Instituto de Investigación Biomédica de Salamanca
2013-2022
Universidad de Salamanca
2008-2022
University of Tolima
2017
Universidade de Sorocaba
2014
Universidad de Oviedo
1996
We confirmed the ability of triterpenoid betulin to protect against neurotoxicity caused by Bothrops jararacussu snake venom in vitro mouse isolated phrenic nerve-diaphragm (PND) preparations and examined its capability vivo protection using rat external popliteal/sciatic nerve-tibialis anterior (EPSTA) preparation. Venom complete, irreversible blockade PND (40 μ g/mL), but only partial (~30%) EPSTA (3.6 mg/kg, i.m.) after 120 min. In PND, preincubation with commercial bothropic antivenom...
Colchicine site ligands with indole B rings are potent tubulin polymerization inhibitors. Structural modifications at the 3-position of 1-methyl-5-indolyl-based isocombretastatins (1,1-diarylethenes) and phenstatins endowed them anchors for further derivatization resulted in highly compounds. The substituted derivatives displayed cytotoxicity against several human cancer cell lines due to inhibition, as shown by cycle analysis, confocal microscopy, inhibitory activity studies promoted...
The effect of four sub-extracts prepared from the lyophilized hydroalcoholic bark Dipteryx alata (Leguminosae-Papilionoideae) dissolved in a methanol-water (80:20) mixture through liquid-liquid partition procedure has been investigated against neuromuscular blockade venom snake Bothrops jararacussu. active CH2Cl2 sub-extract extensively analyzed for its chemical constituents, resulting isolation lupane-type triterpenoids: lupeol (1), lupenone (2), 28-hydroxylup-20(29)-en-3-one (3), betulin...
Abstract The vasorelaxant profile of quercetin 3,7-dimethyl ether, a flavonoid isolated from Croton schiedeanus Schlecht (Euphorbiaceae), was assessed in aortic rings Wistar rats. To gain insight into its structure-activity relationship, we compared this substance with 3,4′,7- trimethyl ether (ayanin), another plant, 3,3′,4′,7-tetramethyl synthesized by us, and quercetin. In addition examined the interaction nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) pathway. According to their...
Snakebite is a neglected disease and serious health problem in Brazil, with most bites being caused by snakes of the genus Bothrops. Although serum therapy primary treatment for systemic envenomation, it generally ineffective neutralizing local effects these venoms. In this work, we examined ability 7,8,3'-trihydroxy-4'-methoxyisoflavone (TM), an isoflavone from Dipteryx alata, to neutralize neurotoxicity (in mouse phrenic nerve-diaphragm preparations) myotoxicity (assessed light microscopy)...
Ovarian cancer (OC) is one of the most common gynecologic neoplasia and has highest mortality rate, which mainly due to late-stage diagnosis chemotherapy resistance. There an urgent need explore new better therapeutic strategies. We have previously described a family Microtubule Destabilizing Sulfonamides (MDS) that does not trigger multidrug-mediated resistance in OC cell lines. MDS bind colchicine site tubulin, disrupting microtubule network causing antiproliferative cytotoxic effects. In...
Traditional medicine of Brazilian central savannah preconizes Dipteryx alata extracts against snakebites venoms. Previous studies have shown the extract this plant's bark antagonizing neuromuscular blockade caused by snake venom in isolated mice diaphragm and phrenic nerve models. In present study, triterpenoids lupeol, lupenone, 28-OH-lupenone betulin, which are constituents plant, were observed using vitro pharmacological tests. These tests showed efficacy all tested (1 mg/5mL)...
The acid fraction of extracts from the aerial part Croton schiedeanus afforded six cis-clerodane type diterpenoids. Two them (1 and 4) are new natural compounds. Structural elucidation was achieved on basis their spectral data.
Fractioning of an extract Valeriana pavonii, a native species used in Colombian folk medicine as tranquilizer, led to the isolation and identification isovaleramide, one active constituents responsible for its central nervous system activity anticonvulsant.Description principle on from pavonii.The purification isovaleramide was carried out by chromatographic techniques. Its structural elucidation determined nuclear magnetic resonance mass spectrometry. Maximal electroshock seizure vivo...
Abstract The antihypertensive activity of eighteen oxazolo[3,2-a]pyridine, fhiazolo[3,2-a]pyridine and pyrido[2,1-b]oxazine derivatives has been evaluated in conscious spontaneously hypertensive rats (SHRs), compared with that nifedipine, used as reference. At a dose 50 mg kg−1 (i.p.) eleven compounds resulted significant reduction mean arterial blood pressure; four the were particularly effective, resulting hypotension more than 6 h after administration an effect was still apparent 24 h....
In this study we evaluated the anti-adrenergic response elicited by ayanin, a flavonoid compound isolated from Croton schiedeanus Schlecht, in pithed rat, and inhibitory effect of NO synthase inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME), its acute toxicity profile mice. rats ayanin (5 - 50 mg/kg i. v.) caused dose-dependent decrease pressor chronotropic responses induced intravenous noradrenaline administration (0.25 μg/kg). This was completely abolished prior treatment with L-NAME...
Abstract Nine lignan derivatives ( 4 ‐ 12 ) have been obtained from (‐)‐yatein by treatment with DDQ and NBS. They showed moderate antineoplastic activity (P‐388, A‐549, HT‐29) compared podophyllotoxin, but some of them a better therapeutic index. None the tested compounds shows antiviral (HSV‐1, VSV) or enzyme inhibitor (ADA, DHFR, GST) activities.