Stroma and the heparin-binding fibroblast growth factor (FGF) family influence normal epithelial cell differentiation in embryonic adult tissues. The role of stromal cells expression isoforms FGF ligand receptor were examined during malignant progression from a differentiated, slowly growing, nonmalignant model rat prostate tumor. In syngeneic hosts, mixture resulted tumors which differentiated growing. absence cells, progressed to independent stroma undifferentiated. independence was...

The growth of isolated epithelial and stromal cells from both androgen-dependent normal rat prostate an androgen-responsive model tumor is androgen-independent. When added to co-cultures separated by a semipermeable membrane, androgen stimulated cell without effect on growth. Northern blot nuclease protection analysis mRNA revealed that specifically expressed androgen-sensitive secreted member the heparin-binding fibroblast factor family [keratinocyte (KGF)/fibroblast factor-7]. KGF was...

To evaluate the diagnostic ability of diffusion-weighted imaging (DWI) and dynamic contrast-enhanced (DCEI) in combination with T2-weighted (T2WI) for detection prostate cancer using 3 T magnetic resonance (MRI) a phased-array body coil.Fifty-three patients elevated serum levels prostate-specific antigen (PSA) were evaluated by T2WI, DWI, DCEI prior to needle biopsy. The obtained data from T2WI alone (protocol A), DWI B), C), plus D) subjected receiver operating characteristic (ROC) curve...

Genistein is a major component of soybean isoflavone and has multiple functions resulting in antitumor effects. Prostate cancer 1 the targets for preventive role genistein. We examined effect genistein on human prostate (LNCaP PC-3) cells. Proliferation both cell lines was inhibited by treatment dose-dependent manner. To obtain gene expression profile LNCaP cells, we performed cDNA microarray analysis. The many genes, including apoptosis inhibitor (survivin), DNA topoisomerase II, division...

Stroma and the heparin-binding fibroblast growth factor (FGF) family influence normal epithelial cell differentiation in embryonic adult tissues. The role of stromal cells expression isoforms FGF ligand receptor were examined during malignant progression from a differentiated, slowly growing, nonmalignant model rat prostate tumor. In syngeneic hosts, mixture resulted tumors which differentiated growing. absence cells, progressed to independent stroma undifferentiated. independence was...

BACKGROUND It is well-known that the incidence of benign prostatic hyperplasia (BPH) increases with aging. The age-dependent changes in ratio serum sex steroid concentrations may play a role BPH development. To clarify relationship between tissue these steroids and age, we established precise method simultaneous quantitative analysis for used this to investigate three major (testosterone, dihydrotestosterone, estradiol) human prostate. METHODS methodology was using castrated rat tissue,...

Abstract Background Prostate cancer frequently metastasizes to bone. The skeletal metastases of prostate origin are osteoblastic rather than osteolytic. Recently, the expression bone morphogenetic proteins (BMPs) in cell lines was detected. present study indicated existence BMP‐7 normal tissue, but its function has not been clarified. mechanism by which causes metastasis is clear. We investigated and ‐6 metastatic tissues clarify biological relationship between BMPs prostatic cancer. Methods...

The system L amino acid transporter (LAT) has an important role in the transport of various acids, and there have been reports about relation this to cancer. Although LATs are highly expressed kidneys, little is known their influence on human renal cancer.To clarify clear cell carcinoma (RCC), we investigated expression mRNAs for LAT1, LAT2, LAT3, LAT4, 4F2hc RCC tissues. these five genes were analyzed by real-time reverse transcription polymerase chain reaction matched sets tumor non-tumor...

Activation of Rho, one the small GTPases, and its major downstream target Rho-kinase (ROCK) promotes development metastasis cancer. We previously showed that elevation Rho ROCK expression was associated with tumor invasion, metastasis, an unfavorable prognosis in patients urothelial cancer bladder or upper urinary tract.We investigated effects a inhibitor on growth, migration, apoptosis cells. also examined phosphorylation RhoA (RhoA activity) by measuring GTP-bound active form assessed to...

Interleukin (IL)-15 is a pleiotropic cytokine that important for innate and adaptive immune cell homeostasis. The expression of IL-15 protein controlled by posttranscriptional mechanisms. Here, we constructed human vector consisting the IL-2 signal peptide, mature peptide-coding sequences, an out-of-frame growth hormone gene. Human prostate cancer cells, PC-3, transfected with this highly secretable form gene, successfully secreted abundant bioactive protein. In nude mice, PC-3 cells...

Abstract Aim: Vitamin D acts as an antiproliferative agent against prostate cells. Epidemiological study has shown that a low level of serum vitamin concentration is risk factor for cancer. via receptor (VDR), and association genetic polymorphisms the VDR gene been reported. In current study, we examined with familial cancer in Japanese population. Methods: We performed case–control consisting 81 cases 105 normal control subjects. Three (BsmI, ApaI TaqI) were by restriction fragment length...

The relationship between the clinicopathological features and molecular changes associated with standardized uptake value (SUV) determined by Positron emission tomography (PET) [18F] fluorodeoxyglucose (18F-FDG PET) in human renal cell carcinoma (RCC) has not been elucidated. On other hand, overactivation of phosphatidylinositol 3'kinase (PI3K), serine/threonine kinase Akt, mammalian target rapamycin (mTOR) pathway detected a variety cancers, including RCC. So far, little is known about SUV...

It has been suggested that prostate homeostasis is regulated indirectly by androgens through stromal-epithelial interactions in part factors from the stromal cells acting on receptors epithelial cells. In this report, role of fibroblast growth factor (FGF)-10 prostatic proliferation was investigated. The expression FGF-10 mRNA apparent primary-cultured cells, but not derived human tissue patients with benign hyperplasia (BPH). mitogenic activity recombinant assessed 5-bromo-2'-deoxyuridine...

It is known that BMP a factor strongly induces bone formation in muscle tissue. However, the expression of BMP-7 not detected normal tissues. Recently, we reported level was significantly higher metastatic lesions than and high related to osteoblastic metastasis. The aim this study investigate gene prostate glandular tissues, newly diagnosed cancer local recurrent tissues.Total RNA extracted from 23 samples 28 We also examined human prostatic epithelial cells (HPECs) under conditions...

To clarify the role of serum soluble T cell regulatory molecules in clear renal carcinoma (CCRCC), we measured levels interleukin-2 receptor (sIL-2R), B7-H3 (sB7-H3), and cytotoxic lymphocyte associated antigen-4 (sCTLA-4) 70 CCRCC patients 35 healthy controls. We investigated correlations between these pathological grade, clinical stage, prognosis CCRCC. also assessed relations among each molecules. As a result, level sIL-2R was significantly higher than controls (<mml:math...

Tumor-associated tissue eosinophilia (TATE) occurs in many kinds of cancer. This study analyzed whether TATE improves the survival penile cancer patients.In 17 patients with cancer, was compared between TATE-positive group and TATE-negative group. Tissue eosinophils were observed by transmission electron microscopy.In all cases, 5-year 72.9% (n = 8) 38.9% 9; P 0.131). In stage III IV, it 60% 5) 0% 5; 0.058). Transmission microscopy images revealed vigorous infiltration eosinophils.It is...

Increased expression of programmed cell death 1 ligand (PD-L1) by tumor cells is thought to be a mechanism through which solid cancers promote immune tolerance. However, the association between PD-L1 and prognosis upper urinary tract urothelial carcinoma (UTUC) remains unknown.We examined immunohistochemical tumor-infiltrating lymphocyte density (TILD) in 79 patients with UTUC who underwent nephroureterectomy. We classified tumors into four types based on combination TILD, studied...

The effects of the new steroidal antiandrogen TZP-4238 on hormone-induced canine prostatic hyperplasia (BPH) were studied in comparison with those chlormadinone acetate (CMA), a used Japan. One- to 2-year-old beagle dogs castrated and administered 75 mg/week androstanediol (A-diol) plus 0.75 estradiol (E2) for 25 weeks. These treated orally placebo, 0.5 mg/kg/day TZP-4238, 0.1 2.5 CMA, respectively, 21 weeks after 4 treatment A-diol E2. Treatment or CMA suppressed growth, growth slightly....

Abstract BACKGROUND The authors evaluated a new age‐adjusted and prostate volume‐adjusted biopsy method for the detection of carcinoma through transperineal transrectal approaches in men with PSA levels 4.1–10.0 ng/mL. METHODS value adjusted was calculated by using following four factors: 1) life expectancy Japanese 1998, 2) volume estimated ultrasonography, 3) tumor doubling time (4 years), 4) that influenced death (20 cc). number sites set at 8–20. Between August, 1999 December, 2001, 100...

Renal cell carcinoma (RCC) is a hypervascular tumour due to high constitutive production of vascular endothelial growth factor (VEGF), which activated by hypoxia-inducible (HIF). Elevated levels cardiovascular peptides, including brain natriuretic peptide (BNP), have been reported in patients with cancer, regardless whether they overt disease. Furthermore, it has demonstrated that hypoxia stimulates BNP an HIF-dependent manner. However, the clinical implications such peptides RCC not...