Michiel van Galen
A5000284197- Agricultural Economics and Policy
- Epigenetics and DNA Methylation
- Energy, Environment, Agriculture Analysis
- Environmental Conservation and Management
- Economics of Agriculture and Food Markets
- Lipid metabolism and biosynthesis
- Wine Industry and Tourism
- Diet and metabolism studies
- Global Trade and Competitiveness
- Organic Food and Agriculture
- Genetic Associations and Epidemiology
- Bioeconomy and Sustainability Development
- Health, Environment, Cognitive Aging
- Genetic Syndromes and Imprinting
- Birth, Development, and Health
- Gene expression and cancer classification
- Agriculture and Rural Development Research
- Cooperative Studies and Economics
- Economic Analysis and Policy
- Genomic variations and chromosomal abnormalities
- Global trade and economics
- Corporate Taxation and Avoidance
- RNA modifications and cancer
- Bioinformatics and Genomic Networks
- Horticultural and Viticultural Research
Wageningen University & Research
2005-2025
Leiden University Medical Center
2011-2024
University of Groningen
2015-2019
University Medical Center Groningen
2015-2019
Ministry of Economic Affairs and Climate Policy
2019
Leiden University
2016
Dutch Blood Transfusion Society
1977
Abstract The methylome is subject to genetic and environmental effects. Their impact may depend on sex age, resulting in sex- age-related physiological variation disease susceptibility. Here we estimate the total heritability of DNA methylation levels whole blood variance explained by common single nucleotide polymorphisms at 411,169 sites 2,603 individuals from twin families, establish a catalogue between-individual methylation. Heritability estimates vary across genome (mean=19%)...
Cells can be primed by external stimuli to obtain a long-term epigenetic memory. We hypothesize that exposure elevated blood lipids prime circulating immune cells through changes in DNA methylation, process may contribute the development of atherosclerosis. To interrogate causal relationship between triglyceride, low-density lipoprotein (LDL) cholesterol, and high-density (HDL) cholesterol levels genome-wide methylation while excluding confounding pleiotropy, we perform stepwise Mendelian...
BackgroundSmoking impacts DNA methylation, but data are lacking on smoking-related differential methylation by sex or dietary intake, recent smoking cessation (<1 year), persistence of from in utero exposure, and effects environmental tobacco smoke (ETS).MethodsWe meta-analysed up to 15,014 adults across 5 cohorts with measured blood using Illumina's EPIC array for current (2560 exposed), quit < 1 year (500 (286 ETS exposure (676 exposed). We also evaluated the interaction diet (fibre,...
Despite high levels of homology, transcription coactivators p300 and CREB binding protein (CBP) are both indispensable during embryogenesis. They largely known to regulate the same genes. To identify genes preferentially regulated by or CBP, we performed an extensive genome-wide survey using ChIP-seq on cell-cycle synchronized cells. We found that 57% tags were within proximal promoters, with overall preference for start end sites. The heterogeneous patterns possibly reflect divergent roles...
Epigenetic change is a hallmark of ageing but its link to mechanisms in humans remains poorly understood. While DNA methylation at many CpG sites closely tracks chronological age, changes relevant biological age are expected gradually dissociate from mirroring the increased heterogeneity health status older ages.Here, we report on large-scale identification 6366 age-related variably methylated positions (aVMPs) identified 3295 whole blood profiles, 2044 which have matching RNA-seq gene...
We tested whether DNA-methylation profiles account for inter-individual variation in body mass index (BMI) and height they predict these phenotypes over above genetic factors. Genetic predictors were derived from published summary results the largest genome-wide association studies on BMI (n ∼ 350,000) 250,000) to date. methylation by estimating probe-trait effects discovery samples them external samples. Methylation associated with older individuals Lothian Birth Cohorts (LBCs, n = 1,366)...
Genome-wide association and fine-mapping studies in 14 autoimmune diseases (AID) have implicated more than 250 loci one or of these diseases. As 90% AID-associated SNPs are intergenic intronic, pinpointing the causal genes is challenging. We performed a systematic analysis to link 460 that associated with AID using transcriptomic data from 629 blood samples. were able 71 (39%) AID-SNPs two nearby genes, providing evidence for part multiple exist. While 54 shared by AID, 17% them do not share...
Educational attainment is a key behavioural measure in studies of cognitive and physical health, socioeconomic status. We measured DNA methylation at 410,746 CpGs (N = 4152) identified 58 associated with educational loci characterized by pleiotropic functions shared neuronal, immune developmental processes. Associations overlapped those for smoking behaviour, but remained after accounting many CpGs: Effect sizes were on average 28% smaller genome-wide significant 11 adjusting 62% never...
To be able to meet the European Union’s energy and climate targets for 2030, all member states need rethink their production use. One potential renewable source is biogas. Its role has been relatively small compared other sources, but it could have a more central solve some specific challenges, e.g., reduce carbon dioxide (CO2) emissions from traffic, or act as buffer balance electricity with consumption. This research analyses how future of biogas business in three case study countries...
We evaluated massive parallel sequencing and long-range PCR (LRP) for rare variant detection allele frequency estimation in pooled DNA samples. Exons 2 to 16 of the MUTYH gene were analyzed breast cancer patients with Illumina's (Solexa) technology. From a pool 287 genomic samples we generated single LRP product, while same was performed on 88 individual resulting products then pooled. Concentrations constituent measured fluorimetry high-resolution melting curve analysis (HR-MCA) products....
Abstract In the culture medium of some human lymphoblastoid cell lines material is released with following properties: (a) hemagglutination reaction IgG‐sensitized erythrocytes; (b) enhancement precipitation DNA‐anti‐DNA complexes; (c) inhibition binding Clq to immune (d) complex lymphocytes; (e) antibody‐dependent lymphocytotoxicity The not identical or rheumatoid factor, it heat resistant (30 min at 56 °C); molecular weight about 100 000 daltons and capable inhibiting antibody production...
This article examines the relationship between firm-level innovation and employment growth for industrial firms in Netherlands. The empirical analysis uses four waves of CIS survey period 2002-2010. It extends literature by making an explicit split expansion effect labour productivity effect. results show that both product process increase therefore induce direct reductions employment. However, these negative effects are more than compensated increases sales, implying innovations In this...
Abstract Integrating multi-omics data into predictive models has the potential to enhance accuracy, which is essential for precision medicine. In this study, we developed interpretable by employing neural networks informed prior biological knowledge, referred as visible networks. These offer insights decision-making process and can unveil novel perspectives on underlying mechanisms associated with traits complex diseases. We tested performance, interpretability generalizability inferring...
We describe an open-source kPAL package that facilitates alignment-free assessment of the quality and comparability sequencing datasets by analyzing k-mer frequencies. show can detect technical artefacts such as high duplication rates, library chimeras, contamination differences in preparation protocols. also successfully captures complexity diversity microbiomes provides a powerful means to study changes microbial communities. Together, these features make attractive broadly applicable tool...
Abstract Background The identification of transcription factor binding sites is difficult since they are only a small number nucleotides in size, resulting large numbers false positives and negatives current approaches. Computational methods to reduce look for over-representation set similarly regulated promoters or conservation orthologous promoter alignments. Results We have developed novel tool, "CORE_TF" (Conserved Over-REpresented Transcription Factor sites) that identifies common...
The price gap between organic and conventional food might explain the low market share of organics in Netherlands. A real-life experiment was carried out 2006 order to determine sensitivity consumer demand for organics. Consumer prices selected products were reduced by up 40% below current levels. elasticity low, because not all consumers perceived reductions. Moreover, offer varieties is limited, as consumer's willingness pay social attributes
Abstract X-chromosome inactivation (XCI), i.e., the of one female X chromosomes, restores equal expression X-chromosomal genes between females and males. However, ~10% show variable degrees escape from XCI females, although little is known about causes XCI. Using a discovery data-set 1867 1398 males replication sample 3351 we that genetic variation at three autosomal loci associated with female-specific changes in methylation. Through cis -eQTL analysis, map these to SMCHD1 / METTL4 , TRIM6...