Abstract Immune infiltration of colorectal cancer (CRC) is closely associated with clinical outcome. However, previous work has not accounted for the diversity functionally distinct cell types that make up immune response. In this study, based on a deconvolution algorithm (known as CIBERSORT) and annotated expression profiles, we comprehensively analyzed tumor‐infiltrating cells present in CRC first time. The fraction 22 subpopulations was evaluated to determine associations between each...

Alternative splicing (AS), as a potent and pervasive mechanism of transcriptional regulatory, expands the genome's coding capacity involves in initiation progression cancer. Systematic analysis alternative colorectal cancer (CRC) is lacking greatly needed.RNA-Seq data corresponding clinical information CRC cohort were downloaded from TCGA portal. Then, java application, known SpliceSeq, was used to evaluate RNA patterns calculate Percent Spliced In (PSI) value. Differently expressed AS...

Abstract Several long noncoding RNAs (lncRNAs) have been identified in various malignant tumors and determined to contribute the process of tumorigenesis, including that colorectal cancer (CRC). Cancer stem cells (CSCs) demonstrated promote expansion maintain invasion metastasis cells, owing their self‐renewal capacity. However, underlying modulation mechanism CSC‐associated lncRNAs CRC remains largely unclear. Using integrated bioinformatic analysis, we a novel lncRNA (lncRNA‐cCSC1) is...

Metastasis is a major threat to colorectal cancer (CRC) patients. We have reported that peroxiredoxin-2 (PRDX2) associated with CRC invasion and metastasis. However, the mechanisms regulating PRDX2 expression remain unclear. investigate whether microRNAs (miRNAs) regulate in progression. Quantitative real-time polymerase chain reaction (qPCR) was used measure microRNA-200b-3p (miR-200b-3p) expression. Immunohistochemistry (IHC) performed detect c-Myc protein levels tissue samples (n = 97)....

Abstract Colorectal cancer is the most common gastrointestinal and causes severe damage to human health. PRDX2 a member of peroxiredoxin family reported have high level expression in colorectal cancer. However, mechanisms by which promotes proliferation are still unclear. Here, results indicated that was upregulated closely correlated with poor prognosis. Functionally, promoted cells. Mechanistically, could bind RPL4, reducing interaction between RPL4 MDM2. These findings demonstrate...

Although the outcome of patients with colorectal cancer (CRC) has improved significantly, prognosis evaluation still presents challenges due to disease heterogeneity. Increasing evidences revealed close correlation between aberrant expression certain RNAs and prognosis. We envisioned that combined multiple types into a single classifier could improve postoperative risk classification add prognostic value current stage system. Firstly, differentially expressed including mRNAs, miRNAs lncRNAs...

Hypoxia microenvironment, as a major feature of solid tumors, increases tumors progression and metastasis. To research whether hypoxia influences the epithelial-mesenchymal transition (EMT) cancer stem cells (CSCs) gastric their cell biological behaviors. Human lines BGC823 SGC7901 were cultivated in different oxygen tensions for proliferation, colony formation, soft agar migration, invasion analyses. Markers EMT (E-cadherin, N-cadherin, Vimentin, Snail) markers CSCs (Sox2, Oct4, Bmi1)...

The present study was to investigate the clinical significance of peroxiredoxin 2 (PRDX2), an oncoenzyme, in development and progression colorectal cancer(CRC).We found levels PRDX2 mRNA protein were higher CRC cell lines than normal human colonic epithelial cells. expression significantly up-regulated lesions compared with that adjacent noncancerous tissues. tissues from 148 226 (65.5%) patients revealed high level contrast only 13 (5.8%) strong staining cases Increased associated poor...

Although, 5-Fluorouracil (5-FU) remains widely used in adjuvant therapy patients with colon cancer, resistance to 5-FU-based chemotherapy is an important reason for treatment failure. Recent studies have reported that enhanced reactive oxygen species (ROS) scavenging system shows drug 5-FU. Peroxiredoxin-2 (PRDX2), member of the ROS system, and may be a potential target promotes chemosensitivity 5-FU cancer. Here, we depleted PRDX2 by PRDX2-shRNA-LV transduction two cancer cell lines found...

Cancer stem cells (CSCs) are a key target for reducing tumor growth, metastasis, and recurrence. Redox status is critical factor in the maintenance of CSCs, antioxidant enzyme Peroxiredoxin 2 (Prdx2) plays an important role development colon cancer. Therefore, we investigated contribution Prdx2 to stemness CSCs. Here, used short-hairpin RNAs Prdx2-overexpression vector determine effects Prdx2. We demonstrated that knockdown reduced self-renewal sphere formation resulted increased...

Although hundreds of colorectal cancer- (CRC-) related genes have been screened, the significant hub still need to be further identified. The aim this study was identify based on protein-protein interaction network and uncover their clinical value. Firstly, 645 CRC patients’ data from Tumor Cancer Genome Atlas were downloaded analyzed screen differential expression (DEGs). And then, Kyoto Encyclopedia Genes Genomes pathway enrichment analysis performed, PPI DEGs constructed by Cytoscape...

Bisdemethoxycurcumin (BDMC) is a demethoxy derivative of curcumin. In this study, human gastric adenocarcinoma xenograft model was generated in vivo using nude mice and BDMC observed to suppress the growth activity tumors, addition improving physical mental capacity mice. An increased number apoptotic cells, decreased ratio B‑cell lymphoma 2 (Bcl‑2)/Bcl‑2‑associated X protein caspase‑3 expression also following treatment with BDMC, indicating that may promote apoptosis tumors via...

Calcium/calmodulin-dependent serine protein kinase (CASK), which localizes at cell-cell adhesion sites and binds to the heparan sulfate proteoglycan syndecan-2, is involved in cell proliferation, cytoskeletal remodeling, migration. To demonstrate role of CASK colorectal cancer (CRC) carcinogenesis, we examined expression its binding syndecan-2 human CRC tissues. The was measured specimens controls from adenomas normal mucosae by immunohistochemical staining Western blot analysis. Syndecan-2...

Peroxiredoxins (Prxs) are novel group proteins with efficient antioxidant capacity, and some of them also have effects on cell proliferation, differentiation, apoptosis, chemotherapy radiotherapy resistance. Altogether six distinct Prxs expressions were investigated in histological samples colorectal neoplasm the distant normal tissues associatedly parameters such as clinical stage lymphnodes metastasis. Normal almost negative for Prxs, except Prx4 (15/32). In cancer tissues, most prominent...

Colorectal cancer is a one of the most common malignancies. Hypoxia-inducible factor 1-α (HIF1-α) and Survivin play important roles in tumor development; however, literature currently contains few reports on relationship between them colorectal cancer. In this study, we investigated effect HIF1-α Immunohistochemical staining was used to detect expression tissue from 32 patients. Colon adenocarcinoma SW480 cells were cultured under normoxia hypoxic conditions, detected by RT-PCR Western...

The etiology of cancer is unclear. Recent studies indicate that some cytokines, such as interleukin (IL)-17, and regulatory T cells are involved in the development cancer. This study aims to detect a subset cell, IL17+Foxp3+ pathogenesis esophageal (Eca). Twelve patients with squamous Eca were recruited this study. surgically removed tissue was collected. Cells isolated from analyzed by flow cytometry. results showed 2–10% tissue-derived CD4+ expressed Foxp3; only 0.2–0.8% non-ca Foxp3....

Although human peroxiredoxin 2 (PRDX2) has been implicated in tumor progression (e.g., invasion and metastasis), little is known regarding its role the epithelial‑mesenchymal transition (EMT) process during tumorigenesis. The present study offers first evidence, to best of our knowledge, that antioxidant enzyme PRDX2 an important regulating EMT process. It was demonstrated overexpression leads changes cell morphology vitro potent inhibition transforming growth factor (TGF)‑β1‑induced...

Colorectal cancer (CRC) is a prevalent worldwide disease in which the antioxidant enzyme peroxiredoxin 2 (PRDX2) plays an important role. To investigate molecular mechanism of PRDX2 CRC, we performed bioinformatics analysis The Cancer Genome Atlas (TCGA) datasets and Gene Expression Omnibus (GEO) DataSets (accession no. GSE81429). Our results suggest that associated with cell-cycle progression autophagy activated by P38 MAPK/FOXO signaling pathway. Using short-hairpin RNA vector, verified...

Colon cancer stem cells (CCSCs) play an important role in facilitating colon occurrence, metastasis and drug resistance. The results of our previous studies confirmed that the well-studied antioxidant gene peroxiredoxin-2 (PRDX2) promotes progression. However, underlying function mechanisms associated with PRDX2 remodeling context CCSCs have remained poorly studied. In present study, we demonstrated is highly expressed CD133/CD44-positive tissues spheroid CD133+CD44+ CCSCs. overexpression...

Since cellular uptake of PEG [poly(ethylene glycol)]-liposomal L-OHP (oxaliplatin) induces bioactive changes in CRC (colorectal cancer), we have investigated its apoptotic effect and anticancer mechanism. Human SW480 cells were treated with PEG-liposomal a caspase-8 inhibitor [Z-IETD-FMK (benzyloxycarbonyl-Ile-Glu-Thr-dl-Asp-fluoromethylketone)]. Apoptosis was measured by FCM (flow cytometry) TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling) assay. Expression...

Annexin A3 (ANXA3) is overexpressed in various cancers and a potential target for cancer treatment. However, clinical implication biological function of ANXA3 colon remain unknown. This study aimed to investigate the relationship between hypoxia-inducible factor 1-alpha (HIF-1α) ANXA3, explore carcinoma.Expression levels HIF-1α human carcinoma specimens cell lines were detected by immunohistochemistry, real-time PCR Western blot analysis. The proliferation cells was examined. Nude mice used...