A5037031013- Analytical Chemistry and Chromatography
- Chromatography in Natural Products
- Phytochemicals and Antioxidant Activities
- Natural product bioactivities and synthesis
- Antioxidant Activity and Oxidative Stress
- Free Radicals and Antioxidants
- Phytochemistry and Biological Activities
- Essential Oils and Antimicrobial Activity
- Computational Drug Discovery Methods
- Plant chemical constituents analysis
- Cholinesterase and Neurodegenerative Diseases
- Biochemical and Structural Characterization
- Botany and Plant Ecology Studies
- Glycosylation and Glycoproteins Research
- Parkinson's Disease Mechanisms and Treatments
- Medicinal Plants and Neuroprotection
- Spectroscopy and Chemometric Analyses
- Traditional Chinese Medicine Analysis
- Metabolomics and Mass Spectrometry Studies
- Neurobiology and Insect Physiology Research
- Mass Spectrometry Techniques and Applications
- Heavy metals in environment
- Plant Gene Expression Analysis
- Nicotinic Acetylcholine Receptors Study
- Plant biochemistry and biosynthesis
University of Alabama
2017-2024
Tuscaloosa City Schools
2019
Medical University of Lublin
2008-2016
National Institute on Aging
2015-2016
Institute on Aging
2016
University of Baltimore
2016
National Institutes of Health
2015-2016
AGH University of Krakow
2016
University of Silesia in Katowice
2015
Krakow University of Economics
2014
The research aims to indicate which of the structural elements monoterpenes are responsible for their antioxidant activity. activity was determined spectrophotometrically with use DPPH• assay. It has been shown that π bonds chain-breaking monoterpenes. proved, first time, blocking conjugated double leads a decrease A probable reaction mechanism between and proposed. indicated strongly depends on polarity solvent used in experiments. presented results may stimulate additional field terpenoid...
Abstract Therapeutics aimed at blocking the cannabinoid 1 (CB1) receptor for treatment of obesity resulted in significant improvements liver function, glucose uptake and pancreatic β-cell function independent weight loss or CB1 blockade brain, suggesting that peripherally-acting only blockers may be useful therapeutic agents. Neuropsychiatric side effects lack tissue specificity precluded clinical use first-generation, centrally acting blockers. In this study we specifically analyzed...
Abstract Alzheimer’s disease (AD) is by far the most prevalent of all known forms dementia. Despite wide-spread research, main causes emergence and development AD have not been fully recognized. Natural, low-molecular, lipophilic terpenoids constitute an interesting group secondary plant metabolites, that exert biological activities possible use in prevention treatment AD. In order to identify metabolites possessing both antioxidant activity potential increase level acetylcholine, selected...
Parkinson's disease is an age-associated neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons from midbrain. Epidemiological studies have implicated exposures to environmental toxins like herbicide paraquat as major contributors etiology in both mammalian and invertebrate models. We employed a paraquat-induced model Drosophila inexpensive vivo platform screen therapeutics natural products. identified polymethoxyflavonoid, GardeninA, with neuroprotective...
The structure of polyphenolic compounds influences their anti-oxidant potential. Finding a simple, rapid and reliable analytical method to study the structure-activity relationships for numerous samples is challenging.To develop simple thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl (TLC-DPPH˙) protocol with image processing influence polyphenols on observed direct properties.First, exhibiting free radical scavenging activities were chosen from among isolated application TLC dot-blot...
Abstract This research aims to investigate synergistic and antagonistic antioxidant interactions for binary mixtures of common monoterpenes. The activity was determined spectrophotometrically with the use DPPH • assay. Significant synergism, between two different monoterpenes, observed a mixture inactive p ‐cymene one compounds, characterized by moderate activity, e.g. (±)‐citronellal, α ‐phellandrene or ‐terpinene. It has been proven that (±)‐citronellal is increased in presence...
The lack of suitable tools for the identification potential drug leads from complex matrices is a bottleneck in discovery. Here, we report novel method to screen new targeting transmembrane receptors. Using α3β4 nicotinic receptors as model system, successfully demonstrated ability this tool specific and effective extraction binding compounds mixtures. formation cell-membrane coated nanoparticles was confirmed by transmission electron microscopy. In particular, have developed direct evaluate...
Abstract Nature-derived bioactive compounds have emerged as promising candidates for the prevention and treatment of diverse chronic illnesses, including neurodegenerative diseases. However, exact molecular mechanisms underlying their neuroprotective effects remain unclear. Most studies focus solely on antioxidant activities natural products which translate to poor outcome in clinical trials. Current therapies against neurodegeneration only provide symptomatic relief thereby underscoring...
For the first time Trifolium scabrum L. was researched for its phenolic profile. Sixteen phenolics (isoflavones and flavonoids) were isolated identified in aerial parts of T. Their structures established by electrospray ionization-mass spectrometry (ESI-MS) nuclear magnetic resonance (NMR) techniques. Quantitative analysis individual performed means ultraperformance liquid chromatography-mass (UPLC-MS) based on calibration curves obtained previously standard compounds. Additionally, free...
Context: The demand for podophyllotoxin and deoxypodophyllotoxin is still increasing commercially exploitable sources are few one of them, Podophyllum hexandrum Royle (Berberidaceae), a "critically endangered" species.Objective: first aim was to quantify the amount in 61 Juniperus (Cupressaceae) samples. Cytotoxic activity ethanolic leaf extracts scopulorum Sarg. "Blue Pacific" communis L. "Depressa Aurea" examined against different leukemia cell lines.Materials methods: Ultra-performance...
Biological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with application HPLC, and recently samples' biological profiles have obtained by means thin-layer chromatography (TLC). This paper summarizes liquid techniques for purpose fingerprint analysis (BFA) complex In case TLC fingerprint, which novel solution, perspectives its further development are outlined more detail. Apart from already published...
Abstract Introduction – Plant‐derived free radical scavengers have become the subject of intensive scientific interest. Recently, concept coupling chromatographic fingerprints with biological fingerprinting analysis has gained much attention for quality control plant extracts. However, identification scavenging activity each single compound in a complex mixture is difficult task. Thin‐layer chromatography post‐chromatographic derivatisation methanol solution DPPH can be valuable tool such...