Abstract Background Rapid diagnostic tests (RDTs) targeting histidine rich protein 2(HRP2) are widely used for diagnosis of Plasmodium falciparum infections. Besides PfHRP2, the PfHRP3 antigen contributes to detection P. infections in PfHRP2 RDTs. However, performance HRP2-based RDT is affected by pfhrp2/3 gene deletions resulting false-negative test results. The objective this study was determine presence and prevalence including respective flanking regions among symptomatic patients Assosa...

: Background: Diversity in Plasmodium falciparum poses a major threat to malaria control and elimination interventions. This study utilized 12 polymorphic microsatellite (MS) markers the Msp2 marker examine diversity, multiplicity of infection (MOI) as well population structure parasites circulating two sites separated by about 92 km with varying transmission intensities within Greater Accra Region Ghana.The diversity MOI P. 160 non-symptomatic volunteers living Obom (high intensity)...

Malaria is a significant public health problem in Ghana. Seasonal Chemoprevention (SMC) using combination of sulfadoxine-pyrimethamine and amodiaquine has been implemented since 2015 northern Ghana where malaria transmission intense seasonal. In this study, we estimated the prevalence asymptomatic P. falciparum carriers three ecological zones Ghana, compared sensitivity specificity different molecular methods identifying infections. Moreover, examined frequency mutations pfcrt,...

In October 2021, the world's first malaria vaccine RTS,S was endorsed by WHO for broad use in children, despite its low efficacy. This study examined polyclonal infections and associations of parasite genetic variations with binding affinity to human leukocyte antigen (HLA). Multiplicity infection determined amplicon deep sequencing PfMSP1. Genetic PfCSP were across 88 samples from Ghana analyzed together 1655 sequences other African non-African isolates. Binding interactions peptide...

Abstract Background Plasmodium vivax Duffy binding protein (PvDBP) is a merozoite surface located in the micronemes of P. . The invasion human reticulocytes by merozoites depends on parasite DBP domain engaging Antigen Receptor for Chemokine (DARC) these red blood cells (RBCs). PvDBPII shows high genetic diversity which major challenge to its use development vaccine against malaria. Methods A cross-sectional study was conducted from February 2021 September 2022 five sites across Ethiopia....

Plasmodium vivax, traditionally overlooked has experienced a notable increase in cases East Africa. This study investigated the geographical origin and genetic diversity of P. vivax Sudan using 14 microsatellite markers. A total 113 clinical samples were collected from two different ecogeographical zones, New Halfa Khartoum, Sudan. Additionally, 841 database incorporated for global analysis to discern relationships among isolates on regional worldwide scales. On scale, our findings revealed...

Abstract Background Genetic diversity in Plasmodium falciparum populations can be used to describe the resilience and spatial distribution of parasite midst intensified intervention efforts. This study microsatellite analysis evaluate genetic population dynamics P. parasites circulating three ecological zones Ghana. Methods A total 1168 afebrile children aged between 3 13 years were recruited from five (5) Primary schools different (Sahel (Tamale Kumbungu), Forest (Konongo) Coastal (Ada...

Abstract Malaria is a significant global health concern, with majority of cases in Sub-Saharan African nations. Numerous antimalarial drugs have been developed to counter the rampant prevalence Plasmodium falciparum malaria. Artemisinin-based Combination Therapy (ACT) has served as primary treatment uncomplicated malaria Ghana since 2005. However, growing concern emerged due escalating reports ACT resistance, particularly Southeast Asia, and its encroachment into Africa. Specifically,...

In malaria-endemic countries, rapid diagnostic tests (RDTs) targeting Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and lactate dehydrogenase (PfLDH) have been widely used. However, little is known regarding the performances of these RDTs in Assosa zone northwest Ethiopia. The objective this study was to determine PfHRP2 PfLDH using microscopy quantitative PCR (qPCR) as a reference test. A health facility–based cross-sectional design conducted from malaria-suspected participants at...

Increases in population and the number of health-care facilities Dakar has led to considerable increase biomedical waste (BMW) generation, posing a huge challenge already burdened city's management system. Following special treatment required for BMW due associated health environmental risks, gap infrastructural development search pathways address challenge, this position paper, examines evolution legal framework wastes management, related issues policy program options city. Historically,...

Background Refugees are vulnerable populations especially in malaria endemic areas where the disease claims many lives and constitutes an emerging challenge for humanitarian response. This study assessed how influx settlement of Central African Republic (CAR) refugees influences burden Gado-Badzere refugee camp, Eastern Cameroon. Methods A cross-sectional malariometric survey was conducted between November 2022 October 2023 324 households comprising 1,304 individuals aged 1 month above....

Abstract Malaria, predominantly caused by Plasmodium falciparum , poses one of largest and most durable health threats in the world. Previously, simplistic regression-based models have been created to characterize malaria rapid diagnostic test performance, though these often only include a couple genetic factors. Specifically, Baker et al., 2005 model uses two types particular repeats histidine-rich protein 2 (PfHRP2) describe P. infection [1], efficacy this has waned over recent years due...

Abstract Malaria, predominantly caused by Plasmodium falciparum , poses one of largest and most durable health threats in the world. Previously, simplistic regression-based models have been created to characterize malaria rapid diagnostic test performance, though these often only include a couple genetic factors. Specifically, Baker et al., 2005 model uses two types particular repeats histidine-rich protein 2 (PfHRP2) describe P. infection, efficacy this has waned over recent years due...

Abstract Background Malaria is a life-threatening infectious disease particularly due to Plasmodium falciparum (P.falciparum ). Histidine-Rich Protein 2 (PfHRP-2) and specific Lactate Dehydrogenase (PfPLDH) based rapid diagnostic test are commonly used for malaria diagnosis in endemic countries where microscopic examination scarce. However, there limited information on the performance of RDT rural semi urban area Assosa zone, Northwest Ethiopia. Thus, aim this study determine PfHRP2 PfPLDH...

Background: In October 2021, the world’s first malaria vaccine RTS,S was endorsed by WHO for broad-use in children, despite low efficacy against Plasmodium falciparum malaria. The explanation is unclear. This study examined associations of parasite genetic variations with binding affinity to human immunological proteins including leukocyte antigen (HLA) and T-cell receptors (TCR) involved RTS,S-induced immune responses.Methods: Multiplicity infections determined amplicon deep sequencing...

We report in this work, an extremely rare and major case of anterior body wall defects included ectopia cordis define by abnormal location heart outside the thorax. This was diagnosed at maternity Ourossogui regional hospital center, Senegal. Any scan performed during pregnancy. Newborn died 10 minutes after birth. Ectopia is related to a possible ventral midline developmental abnormality. It’s associated other abnormalities part pentalogy Cantrell. An X-linked genetic

Malaria, predominantly caused by Plasmodium falciparum , poses one of largest and most durable health threats in the world. Previously, simplistic regression-based models have been created to characterize malaria rapid diagnostic test performance, though these often only include a couple genetic factors. Specifically, Baker et al., 2005 model uses two types particular repeats histidine-rich protein 2 (PfHRP2) describe P. infection, efficacy this has waned over recent years due mutations...

Background The world’s first malaria vaccine RTS,S provides only partial protection against Plasmodium falciparum infections. explanation for such low efficacy is unclear. This study examined the associations of parasite genetic variations with binding affinity to human immunological proteins including leukocyte antigen (HLA) and T cell receptors (TCR) involved in RTS,S-induced immune responses. Methods Multiplicity infections was determined by amplicon deep sequencing merozoite surface...