A5015972302- Neuroscience and Neural Engineering
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Neurological disorders and treatments
- Traumatic Brain Injury Research
- Tissue Engineering and Regenerative Medicine
- Neuroethics, Human Enhancement, Biomedical Innovations
- Silk-based biomaterials and applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Neonatal Respiratory Health Research
- Ethics and Legal Issues in Pediatric Healthcare
- EEG and Brain-Computer Interfaces
- Anesthesia and Neurotoxicity Research
- Hydrogels: synthesis, properties, applications
University of Pennsylvania
2016-2023
California University of Pennsylvania
2020-2021
Berlin-Brandenburger Centrum für Regenerative Therapien
2012
Reestablishing cerebral connectivity is a critical part of restoring neuronal network integrity and brain function after trauma, stroke, neurodegenerative diseases. Creating transplantable axon tracts in the laboratory an unexplored strategy for overcoming common barriers limiting regeneration vivo, including growth-inhibiting factors limited outgrowth capacity mature neurons brain. We describe generation, phenotype, constrained three-dimensional human derived from organoids. These...
Successful cell replacement therapy in the central nervous system (CNS) depends on both transplanted type and delivery method. It was established that differentiated neurons are most desirable source; however, they highly sensitive to dissociation shear; removing them from growth surface inflicts serious damage, rendering less viable for transplantation. Pilot experiments using glass colloids as injectable carriers transplantation adult rat hippocampus have greatly improved neuron survival...
Neuronal degeneration and the deterioration of neuronal communication lie at origin many disorders, there have been major efforts to develop cell replacement therapies for treating such diseases. One challenge, however, is that differentiated cells are challenging transplant due their sensitivity both being uprooted from culture growth support shear forces inherent in implantation process. Here, we describe an approach address these problems. We demonstrate rat hippocampal neurons can be...
Neural transplantation represents a promising approach to repairing damaged brain circuitry. Cellular grafts have been shown promote functional recovery through “bystander effects” and other indirect mechanisms. However, extensive lesions may require direct neuronal replacement achieve meaningful restoration of function. While fetal cortical integrate with the host appear develop appropriate attributes, significant ethical concerns limited availability this tissue severely hamper clinical...
Axon regeneration is negligible in the adult mammalian brain, and thus, white matter damage often leads to permanent neurological deficits. A novel approach for axon repair generation of tracts laboratory setting followed by transplantation these constructs. This article details a human substrate this strategy. Using technique stretch growth, functional cortical are generated from pluripotent stem cells at rates up 1 mm/day. These results form basis potential patient-specific protocol...
<ns4:p>Brain organoids are self-organizing, three-dimensional tissues derived from pluripotent stem cells that recapitulate many aspects of the cellular diversity and architectural features developing brain. Recently, there has been growing interest in using human brain organoid transplantation animal models as a means addressing limitations <ns4:italic>in vitro</ns4:italic> culture, such lack vascularization, to explore potential for neural repair. While substantial debate on ethical...
Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe protocol transplanting forebrain into an injured adult rat visual cortex. This includes surgical details craniectomy, aspiration injury, organoid transplantation, and cranioplasty. platform represents valuable tool investigating the efficacy of as structured grafts For complete on use execution this protocol, please refer to Jgamadze et al.
INTRODUCTION: Human brain organoids have potential to be used as a substrate for repair following injury, however the factors affecting survival and integration of transplanted are unknown. We hypothesized that at an earlier time point would result in improved into motor cortex because their greater growth potential. METHODS: forebrain two timepoints (d20-25, d55-60) were adult rats aspiration injury. The first cohort was survived 1 month post transplantation (MPT, n = 6); second 2 MPT (n...
Summary Reestablishing cerebral connectivity is a critical part of restoring neuronal network integrity and brain function after trauma, stroke, neurodegenerative diseases. Creating transplantable axon tracts in the laboratory novel strategy for overcoming common barriers limiting regeneration vivo , including growth-inhibiting factors limited outgrowth capacity mature neurons brain. We describe generation phenotype three-dimensional human derived from organoid tissue. These centimeter-long...
Reestablishing cerebral connectivity is a critical part of restoring neuronal network integrity and brain function after trauma, stroke, neurodegenerative diseases. Creating transplantable axon tracts in the laboratory novel strategy for overcoming common barriers limiting regeneration vivo, including growth-inhibiting factors limited outgrowth capacity mature neurons brain. We describe generation phenotype three-dimensional human derived from organoid tissue. These centimeter-long...
OBJECTIVES/GOALS: Our goal is to develop a silk fibroin scaffold-based neural tissue construct and characterize it in rat model of cortical injury. We aim optimize the for transplantation, test pharmacologic interventions that may enhance its survival, evaluate integration with host brain. METHODS/STUDY POPULATION: To cell density health, scaffolds varying porosity stiffness were seeded E18 GFP+ neurons imaged at DIV 5. Different seeding methods loads similarly tested. Constructs, loaded an...
ABSTRACT IMPACT: This study will provide the essential characterization of intrinsic neural activity in human brain organoids, both at single cell and network levels, to harness for translational purposes. OBJECTIVES/GOALS: Brain organoids are 3D, stem cell-derived tissues that recapitulate neurodevelopment. However, levy their full potential, a deeper understanding is essential. Here, we present our preliminary analysis maturing forebrain organoids. METHODS/STUDY POPULATION: Forebrain were...