A5100601422- Nerve injury and regeneration
- Bone Tissue Engineering Materials
- Dental Implant Techniques and Outcomes
- Neurogenesis and neuroplasticity mechanisms
- Orthopaedic implants and arthroplasty
- Neurological diseases and metabolism
- Pharmacological Effects of Natural Compounds
- Electrospun Nanofibers in Biomedical Applications
- Nerve Injury and Rehabilitation
- Botulinum Toxin and Related Neurological Disorders
- Genetics and Neurodevelopmental Disorders
- Pain Mechanisms and Treatments
- Immunotherapy and Immune Responses
- Osteoarthritis Treatment and Mechanisms
- Dermatologic Treatments and Research
- Dermatological and Skeletal Disorders
- vaccines and immunoinformatics approaches
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Barrier Structure and Function Studies
- Nuclear Receptors and Signaling
- Hereditary Neurological Disorders
- Herpesvirus Infections and Treatments
- Knee injuries and reconstruction techniques
- Plasma Applications and Diagnostics
Osaka University
2009-2019
Fukuyama Cardiovascular Hospital
1975
Abstract Calcium hydroxyapatite ceramics (CHA) are nontoxic materials, provoke little reaction from tissues, and by virtue of these properties represent a good starting point for creating bone substitutes. Although several porous CHAs have been used clinically, there few reports that CHA is fully replaced newly formed bone, which may be due to its structure the limited connectivity between pores. We recently developed interconnected (IP‐CHA) adopting “foam‐gel” technique. Structural analysis...
Bone morphogenetic proteins (BMPs) regulate many mammalian physiologic and pathophysiologic processes. These bind with the kinase receptors BMPR-I BMPR-II, thereby activating Smad transcription factor. In this study, we demonstrate that neogenin, a receptor for netrins of repulsive guidance molecule family, is BMPs modulates signal transduction. Neogenin was found to directly BMP-2, BMP-4, BMP-6, BMP-7. Knockdown neogenin in C2C12 cells resulted enhancement BMP-2-induced processes...
Schwann cells (SCs) are constituents of the peripheral nervous system. The differentiation SCs in injured nerves is critical for regeneration after injury. Methylcobalamin (MeCbl) a vitamin B12 analog that necessary maintenance In this study, we estimated effect MeCbl on SCs. We showed downregulated activity Erk1/2 and promoted expression myelin basic protein dorsal root ganglion neuron-SC coculture system, myelination was by MeCbl. focal demyelination rat model, remyelination motor sensory...
We developed fully opened interconnected porous calcium hydroxyapatite ceramics having two different pore sizes. One has pores with an average size of 150 microm in diameter, 40-microm interconnecting and 75% porosity (HA150). The other 300 60-100-microm (HA300). Because its smaller HA150 greater mechanical strength than that HA300. These were combined rat marrow mesenchymal cells cultured for 2 weeks the presence dexamethasone. then implanted into subcutaneous sites syngeneic rats harvested...
The present study investigated the surface structure and mechanical properties of repair cartilage generated from a tissue engineered construct (TEC) derived synovial mesenchymal stem cells at six months post-implantation compared to those uninjured cartilage.TEC-mediated was cartilaginous with Safranin O staining, had comparable macro-scale compressive cartilage.However, morphological assessments revealed that superficial zone TEC-mediated more fibrocartilage-like, in contrast middle or...
Background: For peripheral nerve defects, autografting is considered the therapeutic gold-standard treatment. However, this procedure leads to donor-site morbidity. While various artificial conduits have been recently developed, treatment outcome has demonstrated be poorer than that with autograft. In our previous study using a rat sciatic crush injury model, we delivery of electrospun nanofiber sheets incorporating methylcobalamin (MeCbl sheet) local site promoted regeneration. study,...
Neurotropin® (NTP), a non-protein extract of inflamed rabbit skin inoculated with vaccinia virus, is clinically used for the treatment neuropathic pain in Japan and China, although its effect on peripheral nerve regeneration remains to be elucidated. The purpose this study was investigate effects NTP Schwann cells (SCs) vitro vivo, which play an important role regeneration. In SCs, upregulated protein kinase B (AKT) activity Krox20 downregulated extracellular signal-regulated kinase1/2 under...
Abstract To investigate the effects of anchor substitutions in SYT-SSX junction peptide, an HLA-A24 residue (position 9) B peptide (GYDQIMPKK) was substituted to more favorable residues according HLA-A24-binding motif. Among four substitutes constructed, a substitute with isoleucine (termed K9I peptide) most apparently enhanced affinity for molecule. Subsequent vitro CTL induction analysis using PBMCs 15 HLA-A24+ synovial sarcoma patients revealed that original allowed induce...
Objectives: It has been reported that postherpetic neuralgia (PHN) in patients over 60 years of age is aggravated under cold stimulation and often difficult to treat. Keishikajutsubuto (TJ-18) Bushi-matsu (TJ-3022) are traditional Japanese herbal medicines have long used treat arthralgia, which following stimulation. This study was designed evaluate the effectiveness combined TJ-18 TJ-3022 therapy cases PHN by self-reported Design: Fifteen (15) aged were examined. Patients aware persistent...
Abstract Dielectric barrier discharge plasma treatment at low pressure is found to significantly improve the biological performance of artificial bones made interconnected porous calcium hydroxyapatite (IP-CHA). One essential parameters associated with their hydrophilicity exterior surfaces as well inner pores. It that pressures highly effective in making pore more hydrophilic. Preliminary vivo experiments plasma-treated IP-CHA rats have shown fast formation blood vessels pores, implying...
Background: Outcomes of peripheral nerve repair after injury are often suboptimal. Therefore, developing biological approaches to augment regeneration is important. In this in vivo study, we tested the hypothesis that augmentation with an electrospun nanofiber sheet incorporating methylcobalamin (MeCbl) would be effective for transection and repair. Methods: Rats were divided into 3 groups either underwent sciatic or without MeCbl sheet, a sham operation. At 4 and/or 8 weeks operation,...