A5084255066- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Virus-based gene therapy research
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Cancer Immunotherapy and Biomarkers
- Nanoplatforms for cancer theranostics
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Hematopoietic Stem Cell Transplantation
- Periodontal Regeneration and Treatments
- Thyroid Cancer Diagnosis and Treatment
- Sarcoma Diagnosis and Treatment
- Circular RNAs in diseases
- Herpesvirus Infections and Treatments
- Cancer Cells and Metastasis
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Cancer Research and Treatments
- Neuroblastoma Research and Treatments
- interferon and immune responses
- Immunotherapy and Immune Responses
- Colorectal Cancer Treatments and Studies
- Bone fractures and treatments
Integrated BioTherapeutics (United States)
2023-2024
NYU Langone Health
2023
University of Pittsburgh Medical Center
2007-2020
University of Pittsburgh
2013-2018
National Institutes of Health
2018
UPMC Hillman Cancer Center
2016
Children's Hospital of Philadelphia
2004-2007
Children's Hospital of Pittsburgh
2006-2007
Children’s Institute
2004
We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability regeneration abilities. show here cell sex is a variable considerably influences MDSCs' found the female MDSCs (F-MDSCs) regenerated muscle more efficiently. Despite using additional isolation techniques cloning, we could not obtain male subfraction with capacity similar to of...
Stromal progenitor cells (SPC) exhibit immunosuppressive effects in vitro that have led to speculation regarding their capacity evade host immune recognition and treat autoimmune diseases gravt-versus-host disease. However, there is little vivo experimental data support these immunologic claims. To assess of SPC vivo, we evaluated the response animals transplanted with SPC. C57BL/6 (B6) or Balb/c adult, murine, bone marrow-derived (AmSPC) were administered by intraperitoneal injection into...
Muscle-derived cells have been successfully isolated using a variety of different methods and shown to possess multilineage differentiation capacities, including an ability differentiate into articular cartilage bone in vivo; however, the characterization human muscle-derived stem (hMDSCs) their regenerative capacities not fully investigated. Genetic modification these may enhance osteogenic capacity, which could potentially be applied therapies. We found that hMDSCs, by preplate technique,...
Skeletal muscle atrophy in cancer cachexia is mediated by the interaction between stem cells and various tumor factors. Although Notch signaling has been known as a key regulator of both development cell activity, potential involvement concomitant yet to be elucidated. The murine K7M2 osteosarcoma line was used generate an orthotopic model sarcoma-associated cachexia, role evaluated. observed sarcoma-bearing mice, highly active tissues atrophic skeletal muscles. Systemic inhibition reduced...
Current preclinical osteosarcoma (OS) models largely focus on quantifying primary tumor burden. However, most fatalities from OS are caused by metastatic disease. The quantification of currently relies CT, which is limited motion artifact, requires intravenous contrast, and can be technically demanding in the setting. We describe ability for indocyanine green (ICG) fluorescence angiography to quantify a previously validated orthotopic, immunocompetent mouse model.(1) Can near-infrared ICG...
The overall survival rate of patients with osteosarcoma (OS) and pulmonary metastases has remained stagnant at 15-30% for several decades. Disulfiram (DSF) is an FDA-approved aldehyde dehydrogenase inhibitor that reduces the metastatic phenotype OS cells in vitro. Here we evaluate its vivo efficacy, as compared to doxorubicin chemotherapy, a previously-validated orthotopic model OS.All treatment groups displayed significantly reduced quantitative burden controls. Lo DSF-treated animals was...
1604 Background: Diversity in clinical studies is essential to address health disparities, but racial and ethnic minorities are often underrepresented studies, especially precision medicine using genetic data (1). BASECAMP-1 (NCT04981119) an ongoing prescreening study identify patients with unresectable advanced or metastatic solid tumors tumor-associated HLA-A*02 LOH for the EVEREST-1 -2 Tmod chimeric antigen receptor T-cell therapy trials. When enrollment began 2021, eligibility Part 1 was...
<h3>Background</h3> Precision medicine studies must overcome the operational burden of prescreening patients and small cohort sizes for correlative studies. BASECAMP-1 (NCT04981119) is an ongoing study that uses a single next-generation sequencing assay (Tempus AI Inc) to address both problems by: 1) efficiently screening tumor-associated human leukocyte antigen (HLA)-A*02 loss heterozygosity (LOH) subsequent trials Tmod<sup>TM</sup> logic-gated chimeric receptor T-cell (CAR T) therapy<sup>1...
<h3>Background</h3> Chimeric antigen receptor (CAR) T-cell therapy has been challenging in solid tumors due to an absence of tumor-specific targets and the resultant on-target, off-tumor toxicity. Tmod, a novel logic-gated CAR therapy, utilizes blocking discriminate tumor from normal cells, thus mitigating toxicity (figure 1).<sup>1 2</sup> The blocker recognizes human leukocyte (HLA), that is subject LOH.<sup>3 4</sup> Among advanced colorectal, pancreatic, non-small cell lung cancers, HLA...
Abstract Fine-needle aspiration (FNA) of thyroid nodules yields indeterminate cytological diagnosis in ~20% cases, confounding patient management. This includes Hurthle cell nodules, which typically yield Bethesda IV and III cytology. Chromosomal copy number alterations (CNA) are known to occur tumors, particularly carcinomas (HCC) as well other follicular-patterned tumors including papillary (PTC) poorly differentiated (PDTC). The aim this study was evaluate tested positive for CNA but...
Abstract Introduction: The five-year survival of patients with osteosarcoma (OS) lung metastases is as low 15%. Our group has shown that disulfiram, a FDA-approved aldehyde dehydrogenase inhibitor, inhibit proliferation and metastasis in vitro. Here we compare the quantitative effects disulfiram doxorubicin on metastatic OS burden orthotopic mouse model using near-infrared indocyanine green (ICG) angiography. Methodology: In an IACUC-approved protocol, 60 immunocompetent balb/c mice were...
Top of pageAbstract Duchenne muscular dystrophy (DMD) is a devastating X-linked muscle disease characterized by progressive weakness due to the lack dystrophin expression at sarcolemma fibers. Transplantation normal myoblasts into diseased provides donor that fuse with dystrophic fibers and restore dystrophin. This process enables transient delivery improved strength in injected muscle. However, approach has limitations, including immune rejection, poor cellular survival rates, limited...
In this study, we developed several novel tetracycline(tet)-regulated Herpes Simplex Virus-1 (HSV) amplicon vectors by introducing various combinations of tet-dependent transcriptional silencers and activators. Also, to reduce undesirable baseline gene expression, most incorporated insulator elements flanking the expression cassette, which carried Firefly luciferase (FLuc) driven a silencer/activator-responsive promoter. To determine constructs were effective at regulating tested activity...
Top of pageAbstract Primary somatic stem cell isolations tend to be heterogeneous by nature. We have shown that postnatal muscle-derived cells (MDSCs) isolated from a mouse muscle biopsy can undergo multilineage differentiation, and the generated within each given isolation are heterogeneous. From single we isolate various MDSC subpopulations. Although all subpopulations capable not same propensity differentiate toward lineages. When compared regenerative index these assess their myogenic...