A5058470292- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Bone and Joint Diseases
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Orthopaedic implants and arthroplasty
- Adipose Tissue and Metabolism
- Hip and Femur Fractures
- Muscle Physiology and Disorders
- Knee injuries and reconstruction techniques
- Hip disorders and treatments
- Bone health and treatments
- Reconstructive Surgery and Microvascular Techniques
- Shoulder Injury and Treatment
- Bone fractures and treatments
- Phagocytosis and Immune Regulation
- T-cell and B-cell Immunology
- Polymer Surface Interaction Studies
- Fibroblast Growth Factor Research
- Sexual function and dysfunction studies
- Genetic Neurodegenerative Diseases
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Diabetic Foot Ulcer Assessment and Management
University of Iowa
2022-2024
First Affiliated Hospital of Zhengzhou University
2012-2024
University of Pittsburgh
2010-2021
Tianjin Medical University
2020-2021
University of Pittsburgh Medical Center
2017-2019
West Virginia University
2010-2013
Yunnan University
2010
Shanghai Sixth People's Hospital
2006-2008
Shanghai Medical College of Fudan University
2005-2006
NF-κB is a transcription factor activated in response to inflammatory, genotoxic and oxidative stress important for driving senescence aging.Ataxia-telangiectasia mutated (ATM) kinase, core component of DNA damage signaling, activates via posttranslational modifications.Here we demonstrate that ATM senescent cells culture murine tissues from Ercc1-deficient mouse models accelerated aging, as well naturally aged mice.Genetic pharmacologic inhibition reduced activation markers the...
This work describes the performance of a graphene oxide/poly(3,4-ethylenedioxythiophene) nanocomposite material as sensitive and selective electrochemical dopamine sensor.
Human muscle-derived progenitor cells (hMDPCs) offer great promise for muscle cell-based regenerative medicine; however, prolonged ex-vivo expansion using animal sera is necessary to acquire sufficient transplantation. Due the risks associated with use of sera, development a strategy ex vivo hMDPCs required. The purpose this study was investigate efficacy platelet-rich plasma (PRP) hMDPCs. Pre-plated MDPCs, myoendothelial cells, and pericytes are three populations that we isolated by...
Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation and destruction of synovial joints affecting ~7.5 million people worldwide. Disease pathology driven imbalance in the ratio pro-inflammatory vs. anti-inflammatory immune cells, especially macrophages. Modulation macrophage phenotype, specifically M1 to M2, pro- transition, can be induced biologic scaffold materials composed extracellular matrix (ECM). The ECM-based immunomodulatory effect thought...
Abstract Duchenne muscular dystrophy (DMD) is a degenerative muscle disorder characterized by the lack of dystrophin expression at sarcolemma fibers. In addition, DMD patients acquire osteopenia, fragility fractures, and scoliosis indicating that deficiency in skeletal homeostasis coexists but little known about effects on bone other connective tissues within musculoskeletal system. Recent evidence has emerged implicating adult stem cell dysfunction myopathogenesis. Given common mesenchymal...
Muscle-derived stem cells (MDSCs) isolated from mouse skeletal muscle by a modified preplate technique exhibit long-term proliferation, high self-renewal, and multipotent differentiation capabilities in vitro. MDSCs retrovirally transduced to express bone morphogenetic proteins (BMPs) can differentiate into osteocytes chondrocytes enhance articular cartilage repair vivo, feature that is not observed with nontransduced MDSCs. These results emphasize require prolonged exposure BMPs undergo...
Muscle-derived cells have been successfully isolated using a variety of different methods and shown to possess multilineage differentiation capacities, including an ability differentiate into articular cartilage bone in vivo; however, the characterization human muscle-derived stem (hMDSCs) their regenerative capacities not fully investigated. Genetic modification these may enhance osteogenic capacity, which could potentially be applied therapies. We found that hMDSCs, by preplate technique,...
Abstract Platelet‐rich‐plasma (PRP) has attracted great attention and been increasingly used for a variety of clinical applications including orthopedic surgeries, periodontal oral maxillofacial plastic sports medicine. However, very little is known about the antimicrobial activities PRP. PRP found to have properties both in vitro vivo. In vitro, are bacterial‐strain‐specific time‐specific: significantly (80‐100 fold reduction colony‐forming units) inhibits growth methicillin‐sensitive...
SND1 is highly expressed in various cancers. Here, we identify oncoprotein as a previously unidentified endoplasmic reticulum (ER) membrane-associated protein. The amino-terminal peptide of predominantly associates with SEC61A, which anchors on ER membrane. SN domain catches and guides the nascent synthesized heavy chain (HC) MHC-I to ER-associated degradation (ERAD), hindering normal assembly lumen. In mice model bearing tumors, especially transgenic OT-I mice, deletion promotes...
The host immune response to an implanted biomaterial, particularly the phenotype of infiltrating macrophages, is a key determinant biocompatibility and downstream remodeling outcome. present study used subcutaneous rat model compare tissue response, including macrophage phenotype, potential, calcification propensity biologic scaffold composed glutaraldehyde-fixed bovine pericardium (GF-BP), standard care for heart valve replacement, with those electrospun polycarbonate-based supramolecular...
Abstract Implant‐associated infection is a serious complication in orthopedic surgery, and endowing implant surfaces with antibacterial properties could be one of the most promising approaches for preventing such infection. In this study, we developed cefazolin loaded biodegradable polypeptide multilayer nanofilms on implants. We found that amount released tuned. A high local concentration was achieved within first few hours therefore may inhibit bacterial colonization critical...
Implant-associated infection is becoming more and challenging to the healthcare industry worldwide due increasing antibiotic resistance, transmission of resistant bacteria between animals humans, high cost treating infections. In this study, we disclose a new strategy that may be effective in preventing implant-associated based on potential antimicrobial properties platelet-rich plasma (PRP). Due its well-studied for promoting healing, PRP (a biological product) has been increasingly used...
ABSTRACT Duchenne muscular dystrophy (DMD) is the most common seen in children. In addition to skeletal muscle, DMD also has a significant impact on bone. The pathogenesis of bone abnormalities still unknown. Recently, we have identified novel bone-regulating cytokine, fibroblast growth factor-21 (FGF-21), which dramatically upregulated muscles from animal models. We hypothesize that muscle-derived FGF-21 negatively affects homeostasis DMD. Dystrophin/utrophin double-knockout (dKO) mice were...
Osteoarthritis (OA) is a prevalent degenerative joint disease with lack of effective therapeutic. Chondrocyte ferroptosis contributes to the progression OA. PUM2 shown exacerbate ischemia-reperfusion-induced neuroinflammation by promoting ferroptosis, but its role in OA remains unexplored. Here, primary mouse chondrocytes were stimulated IL-1β mimic chondrocyte injury vitro. And was upregulated cartilage tissues and IL-1β-induced chondrocytes. Silencing alleviated inflammation ECM...
Background: Patients presenting with cam deformity of the femoral head and neck sustain repeated trauma to articular cartilage superior acetabulum, chondral delamination injuries found during hip arthroscopy. Two previous studies reveal conflicting chondrocyte viability data in these traumatic injuries. The full-thickness nature flaps may suggest that chondrocytes residing flap matrix joint environment would remain viable despite shear trauma. Hypothesis/Purpose: purpose this study is...
To evaluate a modified free vascularized fibular grafting procedure with an anterior approach to the hip as treatment for femoral neck nonunions.Retrospective radiographic and clinical review.Level I trauma center.Twenty-six nonunions treated between November 2000 December 2005.Harris Hip scoring system standard evaluation bone union.The average follow-up period was 29.3 months (range, 12-63 months). The duration of surgery 2.5 hours 2-4 hours). Average blood loss 300 mL 200-400 mL)....
ABSTRACT Introduction Duchenne muscular dystrophy (DMD) has been well characterized as a disease that affects both skeletal muscle and bone. The pathophysiology responsible for the deficits in bone tissue is still unclear. Methods Quantitative reverse‐transcription polymerase chain reaction Western blot analyses of known myokines from were performed on dystrophic mouse models wild‐type (WT) controls to identify differentially expressed bone‐regulating myokines. Results Twenty‐four 43 myokine...
Three populations of muscle-derived cells (PP1, PP3, and PP6) were isolated from mouse skeletal muscle using modified preplate technique retrovirally transduced with BMP4/GFP. In vitro, the PP1 (fibroblasts) proliferated significantly slower than PP3 (myoblasts) PP6 (muscle-derived stem cells); showed a superior rate survival compared under oxidative stress; chondrogenic capabilities cells. vivo, promoted cartilage regeneration other cell populations. The defects in group had higher...