A5100375767- Pelvic and Acetabular Injuries
- Hip and Femur Fractures
- Epigenetics and DNA Methylation
- Bone fractures and treatments
- Protein Structure and Dynamics
- Spinal Fractures and Fixation Techniques
- Spine and Intervertebral Disc Pathology
- Frailty in Older Adults
- Melanoma and MAPK Pathways
- Machine Learning in Bioinformatics
- Metabolism, Diabetes, and Cancer
- DNA Repair Mechanisms
- Cutaneous Melanoma Detection and Management
- Cancer Immunotherapy and Biomarkers
- Circadian rhythm and melatonin
- Histone Deacetylase Inhibitors Research
- Genomics and Chromatin Dynamics
- Supramolecular Self-Assembly in Materials
- Sirtuins and Resveratrol in Medicine
- Microbial Metabolic Engineering and Bioproduction
- Bone health and osteoporosis research
- Orthopaedic implants and arthroplasty
- DNA and Nucleic Acid Chemistry
- Medical Imaging and Analysis
- Cancer Mechanisms and Therapy
Tianjin Medical University
2014-2024
Tianjin Medical University General Hospital
2011-2021
Tianjin Medical University Cancer Institute and Hospital
2021
Johns Hopkins Medicine
2016
Johns Hopkins University
2016
University of Utah
2015
University of Wisconsin–Madison
2013
Lysine lactylation (Kla) has recently been reported to participate in regulating transcription human cells. However, the characterization, regulatory mechanism and functional consequence of Kla prokaryotes remain unclear. Here, we report that YiaC functions as a lysine lactylase CobB serves delactylase regulation metabolism. We demonstrate catalyzes addition Kla, while erases this PTM both vitro intracellularly. Moreover, show YdiF can catalyze formation lactyl-coenzyme A, which donates...
PHF20L1, a H3K27me2 reader, promotes breast tumorigenesis by cooperating with the PRC2/NuRD complex.
SND1 is highly expressed in various cancers. Here, we identify oncoprotein as a previously unidentified endoplasmic reticulum (ER) membrane-associated protein. The amino-terminal peptide of predominantly associates with SEC61A, which anchors on ER membrane. SN domain catches and guides the nascent synthesized heavy chain (HC) MHC-I to ER-associated degradation (ERAD), hindering normal assembly lumen. In mice model bearing tumors, especially transgenic OT-I mice, deletion promotes...
Mono-segment transpedicular fixation is a method for the treatment of certain types thoracolumbar spinal fracture. Finite element models were constructed to evaluate biomechanics mono-segment Spinal motion (T10–L2) was scanned and used establish models. The superior half cortical bone T12 removed cancellous body assigned material properties injured mimic vertebral Transpedicular T11 performed produce model; L1 fixed short-segment model. Motion differences between functional units von Mises...
PHF8-promoted non-histone methylation switch plays a critical role in replication stress response and genome integrity.
Histone post-translational modifications (HPTMs) provide signaling platforms to recruit proteins or protein complexes (e.g., transcription factors, the so-called "readers" of histone code), changing DNA accessibility in regulation gene expression. Thus, it is an essential task identify HPTM readers for understanding epigenetic regulation. Herein we designed and prepared a novel probe based on self-assembled multivalent photo-cross-linking technique selective enrichment identification...
Post-translational modifications (PTMs) are pivotal in the orchestration of diverse physiological and pathological processes. Despite this, identification functional PTM sites within vast amount data remains challenging. Conventionally, those discerned through labor-intensive time-consuming experiments. Here, we developed an integrated analytical approach for on metabolic enzymes via a screening process. Through gene ontology (GO) analysis, identified 269 with lysine 2-hydroxyisobutyrylation...
The purpose of this study was to employ a new three-dimensional (3D) reconstruction and modeling method measure displacement undisplaced femoral neck fractures (Garden stages I II). We also aimed evaluate the effectiveness Garden classification for determining fractures. A total 120 consecutive patients with were enrolled between 2012 2014, including 60 within group II group. displacements head center (d1) lowest point fovea capitis femoris (d2) rotational (α) in 3D model measured computed...
Cataracts is a misfolding protein disease in which one of the major components γD-crystallin protein. The conformational structure aggregated and interactions that cause aggregation are largely unknown. A recent experimental two-dimensional infrared (2DIR) spectroscopy study determined C-terminal domain has high propensity to form β-sheets whereas N-terminal forms disordered fiber state. We present combined computational molecular dynamics local these domains. computed 2DIR signals agree...
Aβ42 peptides can form helix and sheet structure under different conditions. The conformational conversion is closely associated with Aβ aggregation their neurotoxicity. But the transition from to not be clearly understood. In this study we performed microsecond timescale MD simulations of investigate conformation α-helix β-sheet. Markov state model (MSM) was built facilitate identification crucial intermediate states possible pathway. Based on analysis, found that region Y10-A21 in middle...
Objective To measure the displacement parameters of femoral head in space through CT three-dimensional reconstruction so as to re-understand impacted neck fractures.Methods Fifty patients with fractures were included study.There 17 males and 33 females.Bilateral proximal femurs each patient scanned by spiral preoperatively.Then these primitive data reconstructed Mimics 10.01 software.Registered normal femur fracture mirror model,the registered model was generated a new mask cross-sectional...
The checkpoint kinase ATR is a master regulator of DNA damage response. Yet, how activity regulated remains to be investigated. We report here that histone demethylase PHF8 critically required for activation and cellular response ATR-activating lesions. With combined approach structural biochemical analysis, we unveiled an extraordinary feature the association/dissociation with TOPBP1, essential allosteric activator ATR. removes TOPBP1 K118 mono-methylation facilitate association RAD9, thus...