The resorbable polymers polyglycolic acid (PGA) and polylactic (PLA) are gaining increasing importance in tissue engineering cell transplantation. present investigation was focused on the biocompatibility retaining behavior of PGA/poly-L-lactide (PLLA) (90/10) PLLA nonwoven structures for vitro development chondrocyte-polymer constructs. effect relevant monomers to chondrocytes analyzed. Type II collagen poly-L-lysine were compared improve loading PGA/PLLA polymer nonwovens with...

Cartilage tissue engineering is applied clinically to cover and regenerate articular cartilage defects. Two bioresorbable nonwoven scaffolds, polyglycolic acid (PGA) poly(lactic-co-glycolic acid) (PLGA) (90/10 copolymer of L-lactide glycolide), were seeded with human chondrocytes after initial progeny in a monolayer serum-free medium. subgroups nontreated plasma-treated (using low-pressure plasma technique) scaffolds investigated. The constructs cultivated seeding six-well plates medium for...

Adult adipose-derived stem cells (ASCs) are considered to be an alternative cell source for cell-based cartilage repair because of their multiple differentiation potentials. This article addresses the chondrogenic ASCs seeded into poly-lactide-co-glycolide (PLGA) scaffolds after implantation in a subcutaneous pocket nude mice. Human were PLGA (polylactic acid:polyglycolic acid = 90:10) and cultured transforming growth factor beta 1 (TGF-beta1)-containing medium 3 weeks vitro. Then specimens...

Abstract Resorbable augmentation devices for cruciate ligament surgery have been developed to temporarily protect healing tendon grafts or sutured ligaments against high tensile loads during the postoperative period. Materials available at present [e.g., polydioxanone (PDS)] show a half‐life strength of only 4–6 weeks, whereas process revitalization and recovering transplanted graft can take up 12 months. Therefore, device that provides gradually decreasing mechanical properties with...

We supplemented rat marrow stromal cells (rMSCs) seeded on poly(L-lactic-co-glycolic acid) fiber meshes with transforming growth factor beta1 (TGF-beta1) to improve bone tissue formation for engineering. Whereas our first study (Lieb, E., et al. Tissue Eng. 10, 1399-1413, 2004) investigated the effects of TGF-beta1 matrix and mineralization, this second focused differentiation rMSCs osteoblastic phenotype in dynamic cell culture (orbital shaker). assessed a series markers determine dosing...

We report on the pilot scale synthesis and melt spinning of poly(ethylene furanoate) (PEF), a promising bio-based fiber polymer that can heave mechanical properties in range commercial terephthalate) (PET) fibers. Catalyst optimization solid state polycondensation (SSP) allowed for intrinsic viscosities PEF up to 0.85 dL·g-1. Melt-spun multifilament yarns reached tensile strength 65 cN·tex-1 with an elongation 6% modulus 1370 cN·tex-1. The crystallization behavior was investigated by...

Tissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance conditioning procedures tissue engineered polyurethane prostheses by comparison static and dynamic cultivation methods.Human vascular endothelial cells (ECs) fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) primarily seeded with FBs subsequently ECs, followed different methods cell layers (A: static, B:...

Abstract Since loss of meniscus is correlated with an increasing risk for osteoarthritis, meniscal scaffolds are proposed as new strategies. Development a suitable scaffold has to take into account differing thickness, exposure compressive and tensile forces combined high porosity biocompatibility the material. After physical testing three flat composed different modified polyglycolic acid (PGA) fibers, three‐dimensional meniscus‐shaped PGA‐hyaluronan implant was generated. Micro‐computed...

Abstract Biocompatibility and cell seeding capability of a new scaffold made textured polylactic acid (PLA) fibers was investigated as material for tissue engineering anterior cruciate ligaments (ACL). Adhesion proliferation human mesenchymal progenitor cells (MPC) after 15 days by scanning electron microscopy standard histology. Expression collagen type I III, fibronectin, tenascin C, decorin, smooth muscle actin, the matrix metalloproteinases MMP‐1 MMP‐2, well their inhibitors TIMP‐1...

Bone tissue engineering based on growing bone marrow stromal cells poly(L-lactic-co-glycolic acid) fiber meshes suffers from limited matrix production and mineralization when the are cultured with standard differentiation supplements (dexamethasone, beta-glycerophosphate, ascorbic acid). To overcome this problem we included transforming growth factor beta1 (TGF-beta1), which is described as playing a key role in collagen type I formation, although its effect controversially discussed. The...

The creation of tissue‐engineered constructs with autologous cells is a central goal in regenerative medicine. With respect to ligament replacement, we have evaluated the influences matrix and growth factors on hMSCs (human mesenchymal stromal cells). were seeded two different 3D (three‐dimensional) systems consisting either collagen type I gel or synthetic PLA [poly‐( l ‐lactic acid)] scaffold. After cultivation for 14 days rhTGFβ1 (recombinant human transforming factor β1), rhPDGF‐BB...

Glycosphingolipids are an important component of cell membranes that involved in many biological processes. Fluorescently labeled glycosphingolipids frequently used to gain insight into their localization. However, the attachment a fluorophore glycan part or-more commonly-to lipid is known alter biophysical properties and can perturb function probe. Presented here synthesis novel glycosphingolipid probes with mono- disaccharide head groups ceramide moieties containing fatty acids varying...

Abstract Bacillus anthracis owes its pronounced virulence—apart from specific toxins—to a twofold import mechanism for Fe III ions. This pathogenic bacterium secretes the siderophores bacillibactin (BB) and petrobactin (PB), of which only BB is neutralized by human siderocalin, an abundant lipocalin in plasma. We describe reshaping via combinatorial protein design to bind PB⋅Fe instead BB⋅Fe , with even higher affinity ( K D ≈20 p m ). X‐ray crystallographic analysis resulting “petrocalin”...

Abstract Colchicine is a toxic alkaloid prevalent in autumn crocus ( Colchicum autumnale ) that binds to tubulin and inhibits polymerization of microtubules. Using combinatorial rational protein design, we have developed an artificial binding based on the human lipocalin 2 colchicine with dissociation constant 120 p m , i.e. 10000-fold stronger than tubulin. Crystallographic analysis engineered lipocalin, dubbed Colchicalin, revealed major structural changes flexible loop region forms ligand...

Many patients suffering from end-stage liver disease cannot be transplanted within reasonable time due to the shortage of donor organs. Bioartificial support systems may contribute regeneration or bridging until a graft for transplantation becomes available. Nonwovens with integrated oxygenation capacity have been developed and manufactured by melt blow technology using thermoplastic polyurethane. Capillary membranes were into nonwoven during processing. The polyurethane structures adapted...