A5103170707- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- COVID-19 Impact on Reproduction
- Peptidase Inhibition and Analysis
- Inhalation and Respiratory Drug Delivery
- Pharmacological Effects of Natural Compounds
- Infections and bacterial resistance
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Selenium in Biological Systems
- Inflammasome and immune disorders
- Microbial infections and disease research
- Blood Coagulation and Thrombosis Mechanisms
- Sirtuins and Resveratrol in Medicine
- Diagnosis and treatment of tuberculosis
- interferon and immune responses
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- X-ray Diffraction in Crystallography
- Autophagy in Disease and Therapy
- Microbial Metabolism and Applications
- Cell death mechanisms and regulation
- Heme Oxygenase-1 and Carbon Monoxide
China Agricultural University
2021-2024
Mitophagy is a selective autophagy mechanism for eliminating damaged mitochondria and plays crucial role in the immune evasion of some viruses bacteria. Here, we report that Mycobacterium bovis (M. bovis) utilizes host mitophagy to suppress xenophagy enhance its intracellular survival. M. causative agent animal tuberculosis human tuberculosis. In current study, show induces macrophages, induction impaired by PINK1 knockdown, indicating PINK1-PRKN/Parkin pathway involved induced bovis....
Given the worldwide problem posed by enteric pathogens, discovery of safe and efficient intestinal adjuvants combined with novel antigen delivery techniques is essential to design mucosal vaccines. In this work, we designed poly (lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) codeliver all-trans retinoic acid (atRA), antigens, CpG. To address insolubility adjuvant atRA, utilized PLGA encapsulate atRA form a "nanocapsid" polydopamine. By leveraging polydopamine, adsorbed...
Accumulating evidence indicates that antibodies can protect against some intracellular pathogens. Mycobacterium bovis is an bacterium, and its cell wall (CW) essential for virulence survival. However, the questions of whether play a protective role in immunity M. infection what effects specific to CW have still remain unclear. Here, we report targeting isolated pathogenic strain attenuated bacillus Calmette-Guérin (BCG) could induce protection virulent
Respiratory mucosal immunization is an effective strategy against tuberculosis (TB), and vaccines require adjuvants that can promote protective immunity without deleterious inflammation. Mucosal BCG (Bacille Calmette-Guerin) effective, but it causes a severe inflammatory response in the lung. A novel less cytotoxic vaccine AH-PB containing Mycobacterium (Mtb) cell surface antigens Ag85A HspX (AH), as well polyinosinic-polycytidylic acid (Poly IC) bovine neutrophil β-defensin-5 (B5) were...
Glycerol kinase (glpK) is essential for the first step of glycerol catabolism in Mycobacterium tuberculosis. However, bovis has been known to grow poorly media because a base insertion glpK gene.We analyzed gene sequences 60 clinical M. isolates, and determined minimum inhibitory concentration 14 drugs by microdilution method evaluate effect frameshift mutations on drug sensitivity. The growth rate its sensitivity was investigated using bacteria grown or pyruvate.A total 44 (73.33%) isolates...
Mycobacterium bovis is the cause of bovine tuberculosis, and it can also disease in humans, with symptoms similar to those caused by M. tuberculosis. However, our understanding its genomic diversity, biogeography, drug resistance remains incomplete. We performed a comparative phylogenetic analysis 3228 genomes from 24 countries. Following susceptibility testing, we applied bacterial genome-wide association study capture associations between variation 74 newly isolated strains China. The data...
It has been established that kallikrein12 (KLK12) expression is closely related to bovine tuberculosis (bTB) development. Herein, we sought clarify the regulatory mechanism of KLK12 and its application in diagnosis. knockdown macrophages were produced by siRNA transfection. Bradykinin receptors (BR, including B1R B2R) blocked with specific inhibitors. Mannose-capped lipoarabinomannan (ManLAM) was extracted from Mycobacterium bovis (M. bovis) used study activation. In addition, constructed...
The diagnosis of drug‐resistant tuberculosis (TB) by molecular testing Mycobacterium drug resistance genes is becoming increasingly common clinically. However, M. bovis , as an uncommon pathogen human TB, may interfere with the test results. A comprehensive understanding phylogenetically informative mutations in required to distinguish true resistance‐conferring mutations. We analyzed 53 165 isolated from humans using whole‐genome sequencing data and found that 98.2% (162/165) isolates have...
Abstract The authors have requested that this preprint be removed from Research Square.