A5007890172- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Immune responses and vaccinations
- Advanced Battery Materials and Technologies
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Cell Function and Interaction
- Pharmacological Effects of Natural Compounds
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- COVID-19 Impact on Reproduction
- Advancements in Battery Materials
- Peptidase Inhibition and Analysis
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Immune Response and Inflammation
- Immune cells in cancer
- Diagnosis and treatment of tuberculosis
- interferon and immune responses
- Chemical Synthesis and Characterization
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
Beijing University of Chemical Technology
2025
China Agricultural University
2020-2024
Guangxi University
2024
Given the worldwide problem posed by enteric pathogens, discovery of safe and efficient intestinal adjuvants combined with novel antigen delivery techniques is essential to design mucosal vaccines. In this work, we designed poly (lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) codeliver all-trans retinoic acid (atRA), antigens, CpG. To address insolubility adjuvant atRA, utilized PLGA encapsulate atRA form a "nanocapsid" polydopamine. By leveraging polydopamine, adsorbed...
Accumulating evidence indicates that antibodies can protect against some intracellular pathogens. Mycobacterium bovis is an bacterium, and its cell wall (CW) essential for virulence survival. However, the questions of whether play a protective role in immunity M. infection what effects specific to CW have still remain unclear. Here, we report targeting isolated pathogenic strain attenuated bacillus Calmette-Guérin (BCG) could induce protection virulent
Mycobacterium bovis (M. bovis) is a member of the tuberculosis complex imposing high zoonotic threat to human health. The limited efficacy BCG (Bacillus Calmette–Guérin) and upsurges drug-resistant require new effective vaccination approaches anti-TB drugs. Poly (lactic-co-glycolic acid) (PLGA) preferential drug delivery system candidate. In this study, we formulated PLGA nanoparticles (NPs) encapsulating recombinant protein bovine neutrophil β-defensin-5 (B5), investigated its role in...
Respiratory mucosal immunization is an effective strategy against tuberculosis (TB), and vaccines require adjuvants that can promote protective immunity without deleterious inflammation. Mucosal BCG (Bacille Calmette-Guerin) effective, but it causes a severe inflammatory response in the lung. A novel less cytotoxic vaccine AH-PB containing Mycobacterium (Mtb) cell surface antigens Ag85A HspX (AH), as well polyinosinic-polycytidylic acid (Poly IC) bovine neutrophil β-defensin-5 (B5) were...
Mycobacterium bovis (M. bovis) is a member of mycobacterium tuberculosis complex (MTBC), and causative agent chronic respiratory disease in wide range hosts. Bacillus Calmette-Guerin (BCG) vaccine mostly used for the prevention childhood tuberculosis. Further substantial implications are required development evaluation new (TB) vaccines as well improving role BCG TB control strategies. In this study, we prepared PLGA nanoparticles encapsulated with argF antigen (argF-NPs). We hypothesized,...
Hypoxia-inducible factor-1α (HIF-1α) is a pivotal regulator of metabolic and inflammatory responses. This study investigated the role HIF-1α in Mycobacterium bovis infection its effects on host immune metabolism tissue damage. We evaluated expression immunometabolism markers matrix metalloproteinases (MMPs) cells infected with M. bovis, following inhibition vitro. To understand implications disease progression, mice at different stages were treated inhibitor, YC-1. Our results revealed an...
The role of gut microbiota has been described as an important influencer the immune system. Gut-lung axis is critical in prevention mycobacterium infection, but specific mechanism, by which dysbiosis affects tuberculosis, not reported. In this study, we attempted to provide more information on how gut-lung contributes Mycobacterium bovis (M. bovis) infection. Mice are pre-treated with broad-spectrum antibiotics cocktail (Abx) induce dysbiosis. Interestingly, microbes showed a significant...
Abstract Mycobacterium bovis is the causative agent of bovine tuberculosis and also responsible for serious threat to public health. Koumiss a fermented mare's milk product, used as traditional drink. Here, we explored effect koumiss on gut microbiota host immune response against M infection. Therefore, mice were treated with fresh mare 14 days before infection continue 5 weeks after The results showed clear change in intestinal flora koumiss, lungs severe edema, inflammatory infiltration,...
Introduction: The limited efficacy of BCG (bacillus Calmette-Guérin) urgently requires new effective vaccination approaches for the control tuberculosis. Poly lactic-co-glycolic acid (PLGA) is a prevalent drug delivery system. However, effect PLGA-based nanoparticles (NPs) against tuberculosis induction mucosal immune response no fully elucidated. In this study, we hypothesized that intranasal immunization with culture filtrate protein-10 (CFP10)-loaded PLGA NPs (CFP10-NPs) could boost...
It has been established that kallikrein12 (KLK12) expression is closely related to bovine tuberculosis (bTB) development. Herein, we sought clarify the regulatory mechanism of KLK12 and its application in diagnosis. knockdown macrophages were produced by siRNA transfection. Bradykinin receptors (BR, including B1R B2R) blocked with specific inhibitors. Mannose-capped lipoarabinomannan (ManLAM) was extracted from Mycobacterium bovis (M. bovis) used study activation. In addition, constructed...
The diagnosis of drug‐resistant tuberculosis (TB) by molecular testing Mycobacterium drug resistance genes is becoming increasingly common clinically. However, M. bovis , as an uncommon pathogen human TB, may interfere with the test results. A comprehensive understanding phylogenetically informative mutations in required to distinguish true resistance‐conferring mutations. We analyzed 53 165 isolated from humans using whole‐genome sequencing data and found that 98.2% (162/165) isolates have...
Abstract Paratuberculosis is a chronic wasting disease of granulomatous enteritis in ruminants caused by Mycobacterium avium subsp. paratuberculosis ( M. , MAP) resulting heavy economic losses to dairy industries worldwide. Currently, commercial vaccines were not effective preventing pathogen shedding and reported with serious side effects. To develop novel smarter vaccine, we utilized PLGA nanoparticles encapsulating the Bfra antigen (Bfra-PLGA NPs). We observed that mice vaccinated...
Abstract The authors have requested that this preprint be removed from Research Square.