Serum samples collected during 1978–1979 from residents of the Chiniot and Changa Manga National Forest (CMF) areas Punjab Province, Pakistan, had over-all neutralizing (N) antibody positive rates for West Nile (WN) virus 32·8% (n = 192) 38·5% 239), respectively. Comparison age-specific indicated that pattern exposure to infection was different in two areas. Samples a 1968 serosurvey CMF area an N profile similar 1978 sample, but both haemagglutinationinhibition (HI) were much higher sample....

Background: Therapeutic plasma exchange (TPE) decreases clotting factors and cellular blood components. Choice of replacement fluid can be important factor in outcome therapeutic large volume exchange.ObjectiveThis study aimed to evaluate changes coagulation parameers using thromboelastography (TEG) prior post-plasma with albumin alone or fresh frozen (FFP). DesignThis is prospective comparative study. Materials Methods: A carried out the Department Transfusion Medicine, Indraprastha Apollo...

<b>Background:</b> Patients with thalassemia major are largely transfusion dependent and thus exposed to a variety of risks such as transmission infectious diseases, iron overload alloimmunization. This study was performed determine the prevalence human immune deficiency virus (HIV), hepatitis B (HBV), C (HCV) red cell antibodies among multiple-transfused thalassemic patients in around national capital region. <b>Materials Methods:</b> The Department Transfusion Medicine, Indraprastha Apollo...

Hepatitis B virus (HBV), human immunodeficiency (HIV), hepatitis C (HCV) and syphilis infections pose a great threat to blood safety. This study was undertaken investigate the seroprevalence of serologic markers for transfusion transmitted (TTIs) among donors at hospital based centre in north India over period nine years.The results TTIs (HBsAg, anti-HCV, anti-HIV syphilis) all donations (both voluntary replacement) our from January 2005 December 2013 were screened. Additional analysis...

India has the second highest HIV population in world with about 2.5-3.0 million cases. HIV-2 cases among general and blood donor have also been reported mostly from west south India. This single centre study was carried out to observe HIV-1 prevalence donors north India.A total of 2,04,677 people were screened for presence infection over 11 year period (1999 2009). Till 2004, a third generation ELISA kit used. From 2005 till January 2009 all tests done using fourth which detected P24 antigen...

Abstract The accurate estimation of ABO antibody titers is the utmost importance in organ transplants involving incompatibility. We aim to compare five different methods titration and analyze data. Samples 48 O group blood donors who donated during month December 2015 January 2016 our institution were subjected by methods: immediate spin (IS) tube titer, antihuman globulin phase Coomb's gel card titer after dithiotreitol (DTT) treatment plasma, solid red cell adherence method. mean number...

Until lately, anti-HBc antibodies were considered an effective marker for occult Hepatitis B virus (HBV) infection and have served their role in improving blood safety. But, with the development of advanced tests HBV DNA detection, this regard stands uncertain.

SUMMARY Objective The aim of our study was to determine the incidence and causes ABO typing discrepancies among patients blood donors at centre. Background An accurate interpretation group an individual is utmost importance ensure patient safety good transfusion practices. Methods A retrospective observational carried out in Department Transfusion Medicine hospital from March 2013 December 2015. Records all donor samples were retrieved analysed for discrepancies. Results In total, 135 853 62...

<b>Context:</b> The clinically significant antibodies are those active at 37°C and/or by the indirect antiglobulin test. Most of published literature refers to Lewis blood group system be insignificant, whereas M and N groups associated with variable clinical significance. <b>Aims:</b> aim this study is find frequency significance M, systems. <b>Settings Design:</b> was carried out retrospectively from January 2009 December 2012. <b>Materials Methods:</b> Antibody screening performed solid...

<b>Background:</b> Alloantibodies may be detected in blood donors who have either been transfused previously or female with previous obstetric events. These antibodies can occasionally cause severe transfusion reaction, if a large amount of plasma whole is transfused, as massive transfusions and pediatric patients.<br><b>Aims:</b> The present study aims to assess the prevalence red cell healthy at tertiary care hospital-based bank India.<br><b>Materials And Methods:</b> A total 82,153 donor...

Sir, The field of medicine has since infinity been associated with consistent discoveries thus leading to ever increasing list advancing technologies. When talking about transfusion medicine, the discovery blood group antigens marked revolutionising step in success transfusions. Following ABO by Landsteiner 1901,[1] next most important was that Rh 1939 and Weiner further description haemolytic disease new born Levine Stetson.[2] The antigen itself studied extensively its alleles...

Extensive bleeding associated with liver transplantation is a major challenge faced by transplant surgeons, worldwide.To evaluate the blood component consumption and determine preoperative factors that predict same in living donor (LDLT).This prospective study was performed for 1 year period, from March 2010 to February 2011.Intra- postoperative utilization of components 152 patients undergoing LDLT evaluated patient parameters like age, gender, height, weight, disease etiology, hemoglobin...

<b>Introduction:</b> The Procleix Ultrio Plusassay is a new-generation qualitative <i>in vitro</i> nucleic acid amplification test used to screen for human immunodeficiency virus type 1 (HIV-1) RNA, hepatitis C (HCV) RNA and B (HBV) DNA in blood donors. This study was performed compare the assay with Plus Nucleic Acid Test (NAT) assays. <b>Materials Methods:</b> Ten thousand three hundred two donor samples were run parallel ID NAT using assay. Simultaneously, enzyme-linked immunosorbent...

Introduction . The MIC (MHC class I chain-related) genes are a group of nonclassical MHC genes, located in the 1 region chromosome 6. aim present study was to find prevalence chain-related (MICA) alloantibodies patients undergoing live-related donor renal transplantation and its role short-term graft survival. blood transfusion formation these antibodies also studied. Materials Methods Pretransplant samples allograft were tested for anti-MICA antibodies. Association various demographics,...

HELLP (hemolysis, elevated liver enzymes, low platelet count) syndrome occurs in about 0.5%-0.9% of all pregnancies, but its prevalence is higher patients with severe preeclampsia, accounting for a substantial maternal and perinatal morbidity mortality. According to the latest American Society Apheresis guidelines, Therapeutic plasma exchange (TPE) performed postpartum cases antepartum fall Categories III IV, respectively.Retrospective analysis was done at our tertiary care center from...

The management of patients with aplastic anemia, to an extent, depends on the etiology i.e., inherited or acquired. classical Chromosomal breakage study involves detection chromosomal aberrations (breaks, gaps, rearrangements, radials, exchanges, endoreduplications) in peripheral blood cells after culture a T-cell mitogen and DNA clastogenic (cross-linking) agent, such as diepoxybutane (DEB) mitomycin C (MMC). testing needs be performed laboratory appropriate expertise Fanconi Anemia...

The presence of donor-specific antibodies directed against human leukocyte antigen significantly influences renal transplant because antibody-mediated rejection. We performed the screening pre-renal patients for preformed anti-HLA using anti-human globulin augmented-complement-dependent lymphocytotoxicity crossmatch (AHG-CDCXm), luminex (LumXm) and HLA antibody screening. Seven hundred fifty-four were assessed LumXm. was possible in 325 out 754 cases. All three investigations viz. CDCXm,...

Women with high-risk pregnancies are offered prenatal diagnosis through amniocentesis for cytogenetic analysis of fetal cells. The aim this study was to evaluate the effectiveness rapid fluorescence in situ hybridization (FISH) technique detecting numerical aberrations chromosomes 13, 21, 18, X and Y an Indian scenario.A total 163 samples were received a FISH and/or full karyotype from pregnancies. In 116 both conventional culture techniques getting G-banding applied conjunction test using...